Fast dissolution preparation method for blood culture medium

Abstract

The present invention relates to the technology field of the biomedicine, in particular to a preparation method for the rapid dissolving of a blood culture medium. Firstly, the raw materials of the culture medium are weighed, and the raw materials include that peptone is 10 g, beef extract is 3 g, sodium chloride is 5 g, sodium citrate is 3 g, and agar is 0.5 g; the fast solution is prepared by the preparation method that 24.6 percent of magnesium sulfate solution is prepared to be used as fast solution 1 to be reserved; 0.5 percent of paraaminobenzoic acid solution is prepared to be used as fast solution 2 to be reserved; the mixing is realized by that about 20 ml raw material of the fast solution is taken and 10 ml fast solution is taken to be added into the raw material of the culture medium and agitated into paste, and then 1000ml distilled water is added into to be rocked and mixed, and statically positioned for three to five minutes to obtain the transparent culture medium fluid; the pH value is adjusted to be 7.4; the culture medium fluid is subpackaged in a sealed liquid bottle, and then sterilized in the high pressure; the finished product of the blood culture medium includes the obtaining process that 100 units of penicillase are added in each bottle through an injection syringe or not added, after the sterility test, the blood culture medium is reserved in the room temperature. Compared with the prior art, the present invention eliminates the long time heating dissolving and filter process, saves the preparation time and the filter material, and the operation issimple and convenient.

Description

[technical field] [0001] The invention relates to the technical field of biomedicine, in particular to a method for quickly dissolving and preparing blood culture medium. [Background technique] [0002] It is troublesome to prepare blood culture medium according to the conventional method. Usually there are steps such as weighing, adding water, heating to dissolve, filtering, adjusting pH value, subpackaging, and autoclaving. Especially heating and dissolving takes a long time and the effect is poor. Turbidity affects the adjustment of pH value and the observation of bacterial growth. [Content of the invention] [0003] The purpose of the present invention is to overcome the deficiencies of the prior art, and provide a blood culture medium instant preparation technology. [0004] In order to achieve the above object, a blood culture medium rapid dissolving preparation method is designed, which is characterized in that: (1) the culture medium raw materials of the following...

AI Technical Summary

Problems solved by technology

[0002] It is troublesome to prepare blood culture medium according to the conventional method. Usually there are steps such as weighing, adding water, heating to dissolve, filtering, adjusting pH value, subpackaging, and autoclaving. Especially heating and dissolving takes a long time and the effect is poor. Turbidity affects the adjustment of pH value and the observation of bacterial growth

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