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Preparation technology of feed additive biologically active small peptide

A feed additive and bioactive technology, which is applied in the field of preparation of bioactive small peptides, can solve the problems of rough operation and low industrialization level, and achieve the effects of low production cost, promoting growth and development, and enhancing immune regulation

Inactive Publication Date: 2008-03-26
陈忆凤
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research and industrialization of small peptides mostly focus on small peptides derived from plant proteins, and the research on small peptides derived from animal sources is also involved. However, overall, the level of industrialization is very low and the operation is very rough.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. A preparation process of a feed additive bioactive small peptide, the steps are as follows,

[0027] (1) select marine low-value small miscellaneous fish and / or aquatic product processing scraps as raw materials, put them into a grinder after the raw materials are cleaned, and then put them into a homogenizer with a weight ratio of raw materials: water=1:1 or Make fish paste material in the homogenizer;

[0028] (2) Put the fish paste material in the enzymolysis equipment, then add trypsin with enzyme activity greater than 4000u / g substrate in the fish paste material, the consumption of trypsin is 450u / g substrate; The temperature of the slurry material is controlled at 45°C, and the pH is adjusted to 6 with a hydrochloric acid solution with a concentration of 0.1 mol / l and a sodium hydroxide solution with a concentration of 0.1 mol / l, and the material is stirred during enzymatic hydrolysis, and the enzymatic hydrolysis time is 3 Hour;

[0029] (3) The enz...

Embodiment 2

[0030] Example 2. A preparation process of a feed additive bioactive small peptide, the steps are as follows,

[0031] (1) select marine low-value small trash fish and / or aquatic product processing scraps as raw materials, put them into a grinder after the raw materials are cleaned, and then put them into a homogenizer with a weight ratio of raw materials: water=1:3 or Make fish paste material in the homogenizer;

[0032] (2) Place the fish paste material in the enzymolysis equipment, then add trypsin with an enzyme activity greater than 4000u / g substrate to the fish paste material, the amount of trypsin is 500u / g substrate; The temperature of the slurry material is controlled at 55°C, and the pH is adjusted to 7 with a hydrochloric acid solution with a concentration of 1.0 mol / l and a sodium hydroxide solution with a concentration of 1.0 mol / l, and the material is stirred during enzymatic hydrolysis, and the enzymatic hydrolysis time is 5 hours ;

[0033] (3) The enzymolys...

Embodiment 3

[0034] Example 3. A preparation process of a feed additive bioactive small peptide, the steps are as follows,

[0035] (1) select marine low-value small miscellaneous fish and / or aquatic product processing scraps as raw materials, put them into a shredder after the raw materials are cleaned, and then put them into a homogenizer with a weight ratio of raw materials: water = 1: 1.5 or Make fish paste material in the homogenizer;

[0036] (2) Put the fish paste material in the enzymolysis equipment, then add trypsin with an enzyme activity greater than 4000u / g substrate to the fish paste material, the amount of trypsin is 460u / g substrate; The temperature of the slurry material is controlled at 48°C, and the pH is adjusted to 6.2 with a hydrochloric acid solution with a concentration of 0.3 mol / l and a sodium hydroxide solution with a concentration of 0.3 mol / l, and the material is stirred during enzymatic hydrolysis, and the enzymatic hydrolysis time is 3.5 Hour;

[0037] (3)...

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PUM

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Abstract

The present invention relates to a preparation process for producing feed additive bioactive small-peptide. It is characterized by that said preparation process includes the following steps: using oceanic small misullaneous fishes and offals obtained by processing aquatic products as raw material, cleaning said raw material, using mincing machine to mince said raw material to obtain fish pulp material; placing the fish pulp material into an enzymolysis equipment, and adding trypsinase, controlling temperature at 45-55deg.C, regulating pH value to 6-7 and making enzymolysis for 3-5hr; making enzyme inactivation treatment, filtering and removing impurity, concentrating so as obtain the invented paste-like bioactive small-peptide.

Description

technical field [0001] The invention relates to a production process of a feed additive, in particular to a preparation process of a bioactive small peptide as a feed additive. Background technique [0002] Small peptides are intermediate products produced in the process of protein degradation in animals, and are compounds composed of two or more amino acids connected by peptide bonds. Because of its high absorption, high conversion rate and immunostimulatory effect, small peptide substances can significantly improve the survival rate of animals when added to feed, promote the absorption and utilization of various mineral elements in feed and feed conversion, and promote the synthesis of protein in animals. In order to achieve a large increase in production and efficiency of breeding, and as an animal immune enhancer to enhance the animal's own immunity. [0003] Experimental studies have shown that the absorption of small peptides has incomparable advantages over proteins ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16A23K1/18A23K1/10C12P21/00A23K10/10A23K10/20A23K10/26A23K20/147A23K40/00A23K50/80
Inventor 陈忆凤
Owner 陈忆凤
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