Particle analyzer of sheath-flow impedance method

Abstract

The invention puts forward a sheath flow impedance particle analyzer, comprising a counting cell, a counting circuit, a latter sheath partition cell and a liquid waste partition cell; wherein, the counting cell comprises a former cell and a latter cell; the former cell comprises a particle suspension liquid inlet and a former sheath liquid inlet; the latter cell comprises a latter sheath liquid inlet and a liquid waste outlet; a hole is arranged between the former cell and the latter cell which are connected with the counting circuit through electrode; the latter sheath liquid stored in the latter sheath partition cell can automatically and continuously flow into the latter cell under the gravitation and the internal negative pressure in the latter cell; the latter sheath partition cell can make the internal liquid passageway, which connects the inlet and the outlet of the latter sheath partition cell, insulated by the air; the liquid waste partition cell can make the internal liquid passageway insulated, which connects the inlet and the outlet of the liquid waste partition cell. The invention has the advantages of preventing the reflowing of the sample liquid in the process of detecting, reducing the signal noise effectively, increasing the detecting sensitivity of the particle and avoiding the electromagnet noise introduced to the counting cell from the latter sheath partition cell or the liquid waste partition cell.

Description

technical field [0001] The invention relates to a sheath flow impedance method particle analyzer, which can use the sheath flow impedance method to detect and analyze the number and volume of particles and other applications. Background technique [0002] The use of sheath flow impedance method for particle number and volume detection can be traced back to US patents US 3,793,587 and US 3,810,010 in 1974. Its common basic components are: front pool, back pool, small hole, sample needle, electrode, and detection circuit; the front and back pools are connected by a small hole, the sample needle is coaxial with the small hole, and its outlet is placed at a distance from the front pool. The position near the small hole can input the particle suspension (called sample flow) to the counting cell. The front and rear cells each have a source of conductive liquid without particles, which are called the front sheath flow and the back sheath flow. Sheath flow: The pressure of the fron...

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Problems solved by technology

However, in this way, the liquid path from the sheath fluid storage tank to the counting tank will inevitably act as a conductive line, which will inevitably introduce electromagnetic interference signals into the counting tank, and this signal will be superimposed on the impedance of the small hole. signal, thus making signal identification difficult

In order to reduce interference, long and thin capillary glass tubes must be selected, which increases the application risk; at the same time, in order to reduce interference, it is also necessary to avoid the use of small, sensitive solenoid valves to control the liquid circuit, so as to increase the risk of the liquid circuit. The design is troublesome; moreover, the driving pressure of the back sheath flow in this method

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