Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B

A technology of extract and land money, applied in the field of traditional Chinese medicine, can solve the problems of limited liver, few opportunities for early diagnosis, hidden onset of liver cancer, etc.

Inactive Publication Date: 2008-05-28
徐明 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the effective curative measures for liver cancer are liver lobectomy or liver transplantation for early liver cancer. However, the onset of liver cancer is hidden

Method used

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  • Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B
  • Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B
  • Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1: Properly pulverize 300 g of the Marchantia polymorpha raw material, add 20 times the amount of ethanol (i.e. solvent), extract 3 times at 100° C. for 0.5 hours each time, filter the extract, combine the filtrates, and concentrate To dryness, a crude extract is obtained. The crude extract was extracted successively with petroleum ether, ethyl acetate and n-butanol, the n-butanol extract was distilled under reduced pressure, the solvent was recovered, concentrated to dryness, dissolved in methanol, passed through a silica gel (Qingdao Ocean Chemical Factory) chromatographic column, first It was eluted with 400ml of chloroform, and then eluted with 500ml of methanol. The methanol eluate was concentrated under reduced pressure at 48° C., and the concentrated solution was vacuum-dried to obtain an extract with a total flavonoid content of 86%. The ethyl acetate extract was distilled under reduced pressure, the solvent was recovered, concentrated to dryness, dissol...

Embodiment 2

[0038] Example 2: Properly pulverize 300 g of Marchantia convoluta raw material, add 13 times the amount of ethanol (i.e. solvent), and extract 3 times at 70°C for 1.5 hours each time, filter the extract, and combine the filtrates , concentrated to dryness to obtain a crude extract. The crude extract was sequentially extracted with petroleum ether, ethyl acetate and n-butanol, the n-butanol extract was distilled under reduced pressure, the solvent was recovered, concentrated to dryness, dissolved in methanol, and passed through an AB-8 macroporous adsorption resin column (Nankai University Chemical Industry Co., Ltd. Plant) chromatography column, first wash the column with water, then wash the column with 1000ml ethanol, the ethanol eluent is concentrated under reduced pressure at 46°C, and the concentrated solution is spray-dried to obtain an extract with a total flavonoid content of 90%. The ethyl acetate extract was distilled under reduced pressure, the solvent was recovere...

Embodiment 3

[0039] Example 3 Crumble 300 g of Marchantia paleacea raw material, add 16 times the amount of 80% ethanol aqueous solution (i.e. solvent), extract 3 times at 80°C for 1.0 hour each time, filter the extract, and the filtrate After being combined, they were concentrated to dryness to obtain a crude extract. The crude extract was sequentially extracted with petroleum ether, ethyl acetate and n-butanol respectively, the n-butanol extract was distilled under reduced pressure, the solvent was recovered, concentrated to dryness, dissolved in methanol, passed through an S-8 macroporous adsorption resin column (Nankai University Chemical Industry Co., Ltd. Plant) chromatographic column, first wash the column with water, then wash the column with 1000ml ethanol, the ethanol eluent is concentrated under reduced pressure at 49°C, and the concentrated solution is spray-dried to obtain an extract with a total flavonoid content of 71%. The ethyl acetate extract was distilled under reduced p...

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PUM

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Abstract

The invention discloses two liverwort extracts: liverwort total flavonoids extract and liverwort terpene extract; the invention proves the liver cancer resistance and hepatitis B resistance effects of the two extract parts, and also discloses the application in preparations of the medicines used for treating hepatitis B and liver cancer. The extract method is shown as follows: liverwort raw material is pulverized and 10-20 folds dissolvent is added; the extraction is performed 2-3 times at 50-100 DEG C, 0.5-1.5 hours per time; the extract solution is filtered and concentrated to dry to obtain liverwort crude extract. The crude extract is extracted respectively by ligroin, acetidin and n-butanol. n -butanol extract is vacuum distilled, recovering solvent, condensed to dry; after dissolved by methanol, the crude extract passes through a chromatographic column (filled with silica gel or diatomite) which is firstly washed with chloroform and then with methanol, or passes through macroporous adsorptive resin column which is firstly washed with water, and then eluted with ethanol; methanol or ethanol is decompressed concentration at 40-50 DEG C; the concentrated solution is dried through vacuum drying, spray drying or freeze drying to get extract with total flavonoids content of 70-90%. Acetidin extract solution is decompressed and distilled, then the solvent is recovered and concentrated to dry; after being dissolved by methanol, the liquid passes through a silica gel chromatographic column which is eluted by dichloromethane; the dichloromethane eluent is decompressed and concentrated at 30-40 DEG C; the concentrated solution is dried through vacuum drying, spray drying or freeze drying to get extract with total terpenes content of 70-90%.

Description

technical field [0001] The invention relates to a plant extract and its medical application, in particular to the preparation process of the extract of Radix Glycyrrhizae and its application in the preparation of medicines for treating liver cancer and hepatitis B, belonging to the field of traditional Chinese medicine. Background technique [0002] Plants of the genus Marchantineae belong to the phylum Bryophyta, the class Hepaticae, and the order Marchantiales. Also known as: pus scab grass (Shaanxi), ground duckweed, a group of clouds, ground thorax (Guizhou), ground dragon skin (Tibet), longan grass (Qinling Bashan), Bagulong, rice seaweed (Shaanxi) (see Gao Qian et al. Northeast Liverwort Flora. Beijing: Science Press, 1981; 192). In addition to the distribution of Marchantia polymorpha L. in my country, there are also Marchantia convoluta Gao et Chang, Marchantia paleacea Bert.] and Marchantia diptera Mont. and other 3 species, they are mixed together, difficult to di...

Claims

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Application Information

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IPC IPC(8): A61K36/10A61K9/08A61K9/16A61K9/19A61K9/20A61K9/48A61K9/00A61P35/00
Inventor 徐明肖建波徐晓萌
Owner 徐明
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