Technique for preparing liverwort extract and application of it in preparing medicine for treating liver cancer and hepatitis B

Abstract

The invention discloses two liverwort extracts: liverwort total flavonoids extract and liverwort terpene extract; the invention proves the liver cancer resistance and hepatitis B resistance effects of the two extract parts, and also discloses the application in preparations of the medicines used for treating hepatitis B and liver cancer. The extract method is shown as follows: liverwort raw material is pulverized and 10-20 folds dissolvent is added; the extraction is performed 2-3 times at 50-100 DEG C, 0.5-1.5 hours per time; the extract solution is filtered and concentrated to dry to obtain liverwort crude extract. The crude extract is extracted respectively by ligroin, acetidin and n-butanol. n -butanol extract is vacuum distilled, recovering solvent, condensed to dry; after dissolved by methanol, the crude extract passes through a chromatographic column (filled with silica gel or diatomite) which is firstly washed with chloroform and then with methanol, or passes through macroporous adsorptive resin column which is firstly washed with water, and then eluted with ethanol; methanol or ethanol is decompressed concentration at 40-50 DEG C; the concentrated solution is dried through vacuum drying, spray drying or freeze drying to get extract with total flavonoids content of 70-90%. Acetidin extract solution is decompressed and distilled, then the solvent is recovered and concentrated to dry; after being dissolved by methanol, the liquid passes through a silica gel chromatographic column which is eluted by dichloromethane; the dichloromethane eluent is decompressed and concentrated at 30-40 DEG C; the concentrated solution is dried through vacuum drying, spray drying or freeze drying to get extract with total terpenes content of 70-90%.

Description

technical field [0001] The invention relates to a plant extract and its medical application, in particular to the preparation process of the extract of Radix Glycyrrhizae and its application in the preparation of medicines for treating liver cancer and hepatitis B, belonging to the field of traditional Chinese medicine. Background technique [0002] Plants of the genus Marchantineae belong to the phylum Bryophyta, the class Hepaticae, and the order Marchantiales. Also known as: pus scab grass (Shaanxi), ground duckweed, a group of clouds, ground thorax (Guizhou), ground dragon skin (Tibet), longan grass (Qinling Bashan), Bagulong, rice seaweed (Shaanxi) (see Gao Qian et al. Northeast Liverwort Flora. Beijing: Science Press, 1981; 192). In addition to the distribution of Marchantia polymorpha L. in my country, there are also Marchantia convoluta Gao et Chang, Marchantia paleacea Bert.] and Marchantia diptera Mont. and other 3 species, they are mixed together, difficult to di...

AI Technical Summary

Problems solved by technology

At present, the effective curative measures for liver cancer are liver lobectomy or liver transplantation for early liver cancer. However, the onset of liver cancer is hidden, and there are not many opportunities for early diagnosis. However, liver transplantation is limited in donor livers, and the cost is high, so it is difficult to popularize.

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