Cell strain loading device under three-dimensional cultivation condition

A technology of three-dimensional culture and loading device, which is applied in the field of measuring devices for biomechanical research, can solve problems such as low heat generation and complex structure, and achieve the effects of preventing pollution, stable mechanical loading and easy processing.

Inactive Publication Date: 2008-07-16
NORTHWESTERN POLYTECHNICAL UNIV
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Problems solved by technology

[0005] In order to overcome the deficiencies of the existing technology in terms of complex structure, interference factors such as magnetic field and heat, the present invention provides a cell or tissue strain loading device under three-dimensional culture conditions, which has a simple structure and can change loading amplitude, frequency and other parameters without stopping the machine. Avoids magnetic field interference, generates low heat, and is suitable for mechanical loading experiments of cells under three-dimensional culture conditions

Method used

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  • Cell strain loading device under three-dimensional cultivation condition
  • Cell strain loading device under three-dimensional cultivation condition

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1. Cell strain loading device with bimorph as driver

[0023] Referring to Fig. 1, Fig. 2 and Fig. 3, the device of the present invention includes a piezoelectric ceramic driving power supply, a piezoelectric ceramic sheet driving part, a cell culture unit and a laser displacement sensor deformation detection part. The piezoelectric ceramic driving power supply 12 provides the loading voltage; the piezoelectric ceramic sheet driving part is mainly composed of its core component piezoelectric ceramic sheet 5, electrodes, ceramic sheet fixing device, transfer plunger 7, and porous fixing material; the cell culture unit consists of a substrate 1 It is composed of the culture chamber 6; the deformation detection part of the laser displacement sensor is composed of a laser probe 10 and a computer 11. Among them, the electrode is the lead wire terminal 2, the ceramic sheet fixing device is a piezoelectric ceramic ferrule 4, the porous fixing material is porous poly...

Embodiment 2

[0025]Example 2: Piezoelectric stack driver as a cell strain loading device driven

[0026] Referring to Fig. 4 and Fig. 5, the device of the present invention is basically the same as the device in Embodiment 1, the difference is that the device uses a piezoelectric stack driver 15 as a driving source to provide driving force. The transmission of the displacement is realized through the transmission plunger 16 . Fix the piezoelectric stack driver 15 in the driver bracket 13 with fixing bolts 14 , and connect the driver with a transmission plunger 16 to the vibration output end of the piezoelectric stack driver 15 . The various parts of the drive plunger 16 are connected by a fixed pin 17 . When mechanical stimulation is applied to the cells in the experiment, first adjust the power supply 12 to output the required voltage signal, and drive the piezoelectric ceramic 15 to output the corresponding deformation. Similar to Example 1, the driver uses the transmission plunger 16 t...

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Abstract

The invention discloses a cell strain loading device under a three-dimensional culture condition, a power supply is connected to an electrode of a driving part of a piezoelectric ceramic plate by a drive line, and the electrode is connected with the piezoelectric ceramic plate; the piezoelectric ceramic plate is fixed on a base bottom by a fixed device of the ceramic plate, the back end of a displacement transfer plunger and a displacement output end of the piezoelectric ceramic plate are fixed together, a three-dimensional complex which is composed of a cell-scaffold is positioned at the central position of the bottom part of a culture small chamber, the left and the right are respectively connected with porous fixed material, one side of the porous fixed material is fixed on the wall of the culture small chamber, the other side of the porous fixed material is fixedly connected with the displacement transfer plunger; a laser probe is non-contact arranged at the back end of the transfer plunger and is connected with a computer for the detection of the front and back displacement of the transfer plunger. The invention can load amplitude and frequency and facilitate regulation without machine shutdown; the invention has good operability, high repeat precision, smooth mechanical loading and small error; furthermore, the invention can avoid magnetic field interference.

Description

technical field [0001] The invention relates to a measuring device for biomechanical research, in particular to a loading experimental device for cells under three-dimensional culture conditions to receive mechanical stress stimulation. Background technique [0002] In recent years, with the increasing number of interdisciplinary research between life sciences and other disciplines, biomechanics, as an emerging discipline that applies mechanical principles and methods to quantitatively study mechanical problems in living organisms, has gradually become a research hotspot in the field of biology. Among them, the research on the response of cells to mechanical stimuli is one of the frontier fields of biomechanical research. However, due to the extremely complex environment in the human body, in vivo research in this area has become more difficult. A variety of in vitro cell culture techniques provide the possibility to study the biological effects of mechanical stimuli at the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N3/00C12M1/00C12Q1/02G01B11/16
Inventor 商澎骞爱荣杨鹏飞丁冲王哲
Owner NORTHWESTERN POLYTECHNICAL UNIV
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