Cell strain loading device under three-dimensional cultivation condition

Abstract

The invention discloses a cell strain loading device under a three-dimensional culture condition, a power supply is connected to an electrode of a driving part of a piezoelectric ceramic plate by a drive line, and the electrode is connected with the piezoelectric ceramic plate; the piezoelectric ceramic plate is fixed on a base bottom by a fixed device of the ceramic plate, the back end of a displacement transfer plunger and a displacement output end of the piezoelectric ceramic plate are fixed together, a three-dimensional complex which is composed of a cell-scaffold is positioned at the central position of the bottom part of a culture small chamber, the left and the right are respectively connected with porous fixed material, one side of the porous fixed material is fixed on the wall of the culture small chamber, the other side of the porous fixed material is fixedly connected with the displacement transfer plunger; a laser probe is non-contact arranged at the back end of the transfer plunger and is connected with a computer for the detection of the front and back displacement of the transfer plunger. The invention can load amplitude and frequency and facilitate regulation without machine shutdown; the invention has good operability, high repeat precision, smooth mechanical loading and small error; furthermore, the invention can avoid magnetic field interference.

Description

technical field [0001] The invention relates to a measuring device for biomechanical research, in particular to a loading experimental device for cells under three-dimensional culture conditions to receive mechanical stress stimulation. Background technique [0002] In recent years, with the increasing number of interdisciplinary research between life sciences and other disciplines, biomechanics, as an emerging discipline that applies mechanical principles and methods to quantitatively study mechanical problems in living organisms, has gradually become a research hotspot in the field of biology. Among them, the research on the response of cells to mechanical stimuli is one of the frontier fields of biomechanical research. However, due to the extremely complex environment in the human body, in vivo research in this area has become more difficult. A variety of in vitro cell culture techniques provide the possibility to study the biological effects of mechanical stimuli at the...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the deficiencies of the existing technology in terms of complex structure, interference factors such as magnetic field and heat, the present invention provides a cell or tissue strain loading device under three-dimensional culture conditions, which has a simple structure and can change loading amplitude, frequency and other parameters without stopping the machine. Avoids magnetic field interference, generates low heat, and is suitable for mechanical loading experiments of cells under three-dimensional culture conditions

Images