Molecule detecting method for rapidly identifying salt-tolerant iris species

A molecular detection and salt-tolerant technology, applied in the field of biology, to achieve rapid detection and discrimination, improve efficiency, and reduce errors

Inactive Publication Date: 2008-07-23
NANJING UNIV
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Problems solved by technology

[0006] At present, there are no relevant reports on molecular rapid...

Method used

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  • Molecule detecting method for rapidly identifying salt-tolerant iris species

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Experimental program
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Effect test

Embodiment Construction

[0052] See Figure 1. The example data are leaf samples of 120 Iris plant populations (60 individuals each of salt-tolerant and non-salt-tolerant). According to this technical analysis, the plant discrimination results and accuracy rate:

[0053] Table 120 iris population plant discrimination accuracy % (individual number in brackets)

[0054] population

Salt-tolerant populations

Non-salt-tolerant populations

Salt-tolerant population (n=60)

Non-salt-tolerant population (n=60)

0.98(59)

0.03(2)

0.02(1)

0.97(58)

[0055] Note: 59 of the 60 salt-tolerant individuals in the table were correctly identified as a salt-tolerant population, and only 2 individuals were correctly identified as a non-salt-tolerant population; 58 of the 60 non-salt-tolerant individuals were correctly identified as a non-salt-tolerant population, and only 1 Individuals were assigned to salt-tolerant populations.

[0056] The 9 primer sequen...

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Abstract

The invention relates to a detecting and distinguishing method of molecule biology in the biology field, which is characterized in that: DNA is extracted from the aimed plant iris, the nine special primer sequences are processed with DNA expansion to acquire microsatellite molecule marker data, the molecule maker date is analyzed by using the software Arlequin 3.11 to acquire the plant population and classification. The detecting and distinguishing method of molecule biology has the advantages that: the molecule detecting and distinguishing can be carried out fast to judge the iris belongs to the salt tolerant or non-salt tolerant population, the detecting and distinguishing method provides microstellite molecule of the iris salt tolerant population to mark the nine primer sequence and expansive conditions and parameters.

Description

1. Technical field [0001] The invention belongs to the detection and discrimination method of molecular biology in the field of biology. In particular, it involves the molecular detection and identification of salt-tolerant plant populations in the genus Iris, and also involves the detection of genetic diversity, evolution, genetic map construction and QTL (quantitative trait loci) detection of plants in the genus Iris. 2. Background technology [0002] Iris is one of the earliest and most prestigious flowers in gardening. It has rich colors, various flower shapes, different sizes, and strong stress resistance, so it has high ornamental value. Most species of iris cannot grow on saline-alkali soil, or their growth is limited or even dead after being subjected to salt stress. One of them is called salt-tolerant iris, which not only has the above advantages but also has a very strong salt-tolerant ability, and can grow and reproduce normally on saline-alkali land with a salt ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 何祯祥
Owner NANJING UNIV
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