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Use of starch in preparation of chromatographic separating medium

A technology of chromatographic separation and starch, which is applied in other chemical processes, chemical instruments and methods, etc., can solve the problems that starch is used as a chromatographic separation medium and has not been reported.

Inactive Publication Date: 2008-10-08
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] As far as the inventor knows, starch is used as chromatographic separation medium and has no report

Method used

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  • Use of starch in preparation of chromatographic separating medium
  • Use of starch in preparation of chromatographic separating medium
  • Use of starch in preparation of chromatographic separating medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: to the separation of pigment

[0021] Bromocresol Green 0.0015g, Sudan III 0.0045g, 100 mesh wheat starch 15g were packed in the column.

[0022] Dissolve bromocresol green and Sudan III pigments in a test tube with a small amount of methanol, and load the sample on the starch column by wet method, and the eluent is methanol. The two pigment solutions were collected separately in Erlenmeyer flasks, and the separation effect was as follows: Figure 1 to Figure 4 As shown, it can be seen that using wheat starch, bromocresol green and Sudan III can be effectively separated.

Embodiment 2

[0023] Embodiment 2: to the separation of rutin, quercetin

[0024] Put 15g of sweet potato starch in the column, beat evenly, mix 5mg of rutin with 5mg of quercetin, dissolve in methanol, and load the sample by wet method. First use ethyl acetate as the eluent, cooperate with TLC inspection, and when there are no spots of quercetin, change methanol as the eluent, collect the eluent, cooperate with TLC inspection, and stop the elution when there are no spots of rutin.

[0025] Developing agent: n-butanol-acetic acid-water=4:1:5 (above)

[0026] Chromogen: iodine vapor fumigation

[0027] like Figure 5 As shown, 1 is the rutin reference substance; 2 is the quercetin reference substance; 3 is the rutin MeOH eluent; 4 is the quercetin EtOAc eluent.

Embodiment 3

[0028] Embodiment 3: Separation of harpagoside and harpagoside

[0029] Put 15g of potato starch in the column, beat evenly, mix 5mg of harpagoside with 5mg of harpagoside, dissolve in methanol, and load the sample by wet method. First use ethyl acetate as the eluent, cooperate with TLC inspection, and when there are no harpagoside spots, change methanol as the eluent, collect the eluate, cooperate with TLC inspection, and stop the elution when there are no harpagoside spots.

[0030] Developing solvent: ethyl acetate-ethanol-water=4:1:0.5

[0031] Chromogen: 2% vanillin sulfuric acid solution

[0032] like Figure 5 As shown, 1 is the reference substance of harpagoside; 2 is the reference substance of harpagoside; 3 is the eluent of harpagoside MeOH; 4 is the eluent of harpagoside EtOAc.

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Abstract

The invention discloses an application of starch in preparing chromatography separation medium. After fermented and granulated, the starch can be used as an adsorption carrier in the technical field of chromatogram and be used for separating the natural product of polyfunctional groups with small polarity and less water in flow phase. The starch has the advantages of low price, being easy to get, safety and environmental protection and stable performance. As a sorbent, the starch can bring more selectivity for separating and refining compound.

Description

technical field [0001] The invention relates to the application of starch as a chromatographic separation medium, especially for the separation of multifunctional natural products with low polarity and low water content in the mobile phase, and other chemical, pharmaceutical, food, cosmetic and other products. Background technique [0002] In modern biochemical preparation technology, chromatographic separation occupies a core position, and in the field of natural products, it can be used to separate various primary metabolites such as amino acids, nucleotides, monosaccharides, organic acids, fatty acids; secondary metabolites such as alkaloids, terpenes Classes, essential oils, glycosides, pigments; various biological macromolecules such as proteins, polypeptides, enzymes, nucleic acids, polysaccharides. [0003] Chromatographic methods can be divided into adsorption chromatography, partition chromatography, ion exchange chromatography, gel filtration (molecular sieve) chro...

Claims

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Application Information

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IPC IPC(8): B01J20/281
Inventor 孙文基肖琳
Owner NORTHWEST UNIV