Extracting method of chondroitin sulfate

A technology of chondroitin sulfate and extraction method, which is applied in the field of biochemical products, can solve the problems of chondroitin sulfate, such as large damage, cumbersome production steps, low yield, and low purity, so as to reduce difficulty and energy consumption, simplify the production process, single strong effect

Inactive Publication Date: 2008-10-08
SHANDONG TOPSCI BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To sum up, the problems in the current production process include: cumbersome production steps, large amounts of alkali, acid, and ethanol, high energy consumption, large damage to chondroitin sulfate, poor activity, and low yield and purity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Put the white and clean cartilage pieces into the reaction tank, and add an appropriate amount of water to soak for 4 hours;

[0021] (2) Heat up to 58°C, keep the pH at 8.0-8.5, add 4% alkaline endoprotease to the reaction tank, and perform enzymolysis for 8 hours; adjust the pH to 6.0-6.5, heat up to 90°C, and settle for 3 Hour;

[0022] (3) Add 1% diatomaceous earth to filter after the enzymolysis solution is cooled, and the filtrate after press filtration is put into the ultrafiltration tank, and the molecular aperture is 2×10 4 Ultrafiltration membrane ultrafiltration, adding 0.9% NaCl during the ultrafiltration process, the concentrated solution is poured into the sedimentation tank, and the weight ratio of adding and concentrated solution is 1% NaCl;

[0023] (4) The concentrated solution adjusts the pH to 10.5-11.5, adding 20% ​​H with a weight ratio of 5-10% to the concentrated solution 2 o 2 The solution was left to stand at 55-60°C for 8 hours, then th...

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PUM

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Abstract

The invention discloses an extraction method of chondroitin sulfate, belonging to the biological and chemical product field. The method adopts single enzymolysis technology to replace the traditional alkaline hydrolysis process and makes use of ultra-filtration membrane filtration to substitute for repeated alcohol precipitation so as to simplify the production process, ensure the integrity of chondroitin sulfate and increase the yield and purity, with the yield more than 37% and the purity more than 99.5%.

Description

【Technical field】 [0001] The invention relates to a biochemical product, in particular to a production process for directly extracting chondroitin sulfate from animal cartilage. 【Background technique】 [0002] Chondroitin sulfate is an acidic mucopolysaccharide extracted from animal cartilage. It is mostly present in soft tissues such as cartilage, throat bone, nasal middle bone, and trachea of ​​animals. It is composed of mucopolysaccharide molecular chains and belongs to glycosaminoglycan. It is composed of D-glucuronic acid and acetylhexosamine (glucose and galactose) connected by 1,3-β-glycosidic bonds, and has the function of removing lipids and lipoproteins in the blood in the body and blood vessels around the heart. Cholesterol, prevent and treat atherosclerosis, promote cell metabolism, anti-coagulation, anti-inflammation, accelerate wound healing and anti-tumor and other pharmacological effects. It is mainly used clinically for the treatment of hyperlipidemia, arte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 郑扶桑卢言松
Owner SHANDONG TOPSCI BIO TECH
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