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Volume metering method

A volume measurement and volume technology, applied in the field of volume measurement, can solve the problems of inaccurate volume measurement, generation of air bubbles, and incomplete emptying of the volume metering tube of the blood cell analyzer, so as to avoid aggregation, complete emptying, and eliminate adverse effects The bubble effect

Active Publication Date: 2008-10-15
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0016] The purpose of the present invention is to solve the problem of inaccurate volume measurement in the blood cell analyzer, specifically to solve the problem of inaccurate volume measurement of the volume meter tube due to incomplete emptying, and further solve the problem of inaccurate volume measurement of the blood cell analyzer

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0041] For the volume metering device of this embodiment, the layout of its liquid circuit can be found in figure 1 shown. The present invention ensures that the volumetric tube is completely emptied through a new auxiliary method for emptying the volumetric tube; at the same time, a new counting start method is used to ensure that even if there is liquid residue at the beginning of counting, the liquid will not jump up, thereby ensuring No air bubbles affect the performance of counting during counting.

[0042] The specific process of emptying the volume metering tube and volume measurement is as follows: Figure 5 , 6 shown, including the following steps:

[0043] Step 1: Establish a negative pressure, open the fifth two-way valve 6, and the first liquid pump 7 to establish a certain negative pressure in the vacuum chamber 8, and the pressure detection sensor 20 has been monitoring the pressure in the vacuum chamber 8;

[0044] Step 2: Empty the volume metering tube, ope...

Embodiment 2

[0052] The liquid path layout of this embodiment is as follows Figure 7 shown, is actually in the original figure 1 Some changes have been made on the basis of , specifically, the sixth two-way valve 22 and the seventh two-way valve 23 are used instead of the first three-way valve 2 in FIG. 4 . That is, the vacuum chamber 8 is connected to the three-way head 21 at the lower end of the volumetric tube 12 through the seventh two-way valve 23 , and the vacuum chamber 8 is also connected to the upper end of the volumetric tube 12 through the sixth two-way valve 22 . Under this pipeline layout, the same effect of the present invention can also be achieved.

[0053] Similarly, the emptying process of the volume metering tube 12 in this embodiment includes the following steps:

[0054] Step 1: Open the fifth two-way valve 6 and the first liquid pump 7 to establish a certain negative pressure on the vacuum chamber 8;

[0055] Step 2, open the seventh two-way valve 23 and the secon...

Embodiment 3

[0059] The liquid path layout of this embodiment is as follows Figure 8 As shown, some changes are actually made on the basis of the original figure 4, specifically, the second three-way valve 24 is used instead of the second two-way valve 3 in Figure 4 . The common end of the second three-way valve 24 is connected to the upper end of the volume metering pipe 12 , the normally open end is connected to the outside atmosphere through the air filter 19 , and the normally closed end is connected to the normally closed end of the first three-way valve 2 . Under this pipeline layout, the same effect of the present invention can also be achieved.

[0060] Similarly, the emptying process of the volume metering tube 12 in this embodiment includes the following steps:

[0061] Step 1: Open the fifth two-way valve 6 and the first liquid pump 7 to establish a certain negative pressure on the vacuum chamber 8;

[0062] Step 2, open the first three-way valve 2 and the second three-way va...

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Abstract

The invention discloses a volume metering method, comprising the steps of generating negative pressure, emptying a volume metering pipe and metering volume, wherein the step of generating negative pressure is to generate negative pressure in a vacuum chamber through an emptying device; the step of emptying the volume metering pipe is to empty the liquid in the volume metering pipe under the action of the negative pressure of the vacuum chamber through the emptying device, and to keep the lower end of the volume metering pipe communicated with negative pressure all the time in the process of emptying the volume metering pipe; the step of metering volume is to meter the volume of the liquid to be metered through a volume metering device. The adoption of the volume metering method in the technical proposal of the invention can effectively prevent residual liquid from gathering on a pipe joint at the lower end of the volume metering pipe and even producing a liquid film in the prior art, thereby ensuring that the volume metering pipe is emptied more thoroughly, so as to avoid producing bubbles in the beginning stage of counting and effectively eliminate the bubbles having adverse effect on measurement results.

Description

technical field [0001] The invention relates to the technical field of cell detection and analysis, in particular to a volume measurement method. Background technique [0002] A blood cell analyzer that uses the impedance method for cell detection needs to dilute a certain amount of blood sample at a specified ratio with a diluent to measure WBC (white blood cells), RBC (red blood cells), PLT (platelets) and HGB (hemoglobin) and other parameters. The diluted samples of WBC and RBC counting pools pass through their respective counting microholes under negative pressure (the electrodes are inserted into the liquid on both sides of the counting hole, and an electric field environment is established around the two sides of the counting hole. According to the non-conductivity of blood cells When a cell passes through the counting hole, it will cause a change in impedance, the size of the impedance change is proportional to the cell volume), through the pipeline into the volumetr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01F17/00G01N33/49
Inventor 赵丙强甘泉孙承伟
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
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