Method for preparing small-molecular weight hyaluronic acid by adding hyaluronate lyase in course of fermentation

A technology of hyaluronidase and hyaluronic acid, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of reduced viscosity, improved purification efficiency, and simple methods

Inactive Publication Date: 2008-10-29
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, hyaluronidase is added to degrade hyaluronic acid during the fermentation process to increase dissolved oxygen and then increase the yield of hyaluronic acid, which has not been reported in the literature

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Example 1: A hyaluronidase addition strategy was adopted for fermentation in a fermenter, and other conditions were the same as those in the control example. Hyaluronidase (0.05 g / L) was added at 8 h of fermentation. At the end of fermentation, the yield of hyaluronic acid increased to 6.0 g / L, and the molecular weight was 100 kDa.

Embodiment 2

[0010] Example 2: The hyaluronidase addition strategy was adopted for fermentation in a fermenter, and other conditions were the same as those in the control example. Hyaluronidase (0.15 g / L) was added at 8 h of fermentation. At the end of the fermentation, the yield of hyaluronic acid increased to 6.5g / L, and the molecular weight was 60kDa.

Embodiment 3

[0011] Example 3: The hyaluronidase addition strategy was adopted for fermentation in a fermenter, and other conditions were the same as those in the control example. Hyaluronidase (0.25 g / L) was added at 8 h of fermentation. At the end of fermentation, the yield of hyaluronic acid increased to 7.0 g / L, and the molecular weight was 20 kDa.

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PUM

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Abstract

The invention discloses a method for producing hyaluronic acid with small molecular weight by adding hyaluronidase in fermentation process, which belongs to the technology field of the application of enzymolysis reaction in optimization of fermentation process. In the method, hyaluronidase (0.05 to 0.25 g / L) is added in the fermentation process (8 h) to degrade hyaluronic acid, so that the viscosity of a fermentation broth decreases from 300 cp of a reference sample (without hyaluronidase) to 50 to 150 cp, the dissolved oxygen concentration is improved from 0 to 1% of the reference sample to 10 to 15%, the output of hyaluronic acid is improved from 5.0 g / L of the reference sample to 6.0 to 7.0 g / L, and the molecular weight of the hyaluronic acid decreases from 1,300 kDa of the reference sample to 20 to 100 kDa. The inventive method is simple and efficient, and has important industrial application value. The decrease of the viscosity of the fermentation broth results in the improvement of downstream purification efficiency and the reduction of production cost. The hyaluronic acid with small molecular weight produced by the method can be applied to the fields of medicines and healthy food.

Description

technical field [0001] A method for producing low molecular weight hyaluronic acid by adding hyaluronidase in the fermentation process belongs to the technical field of the optimization application of enzymatic hydrolysis reaction in fermentation process. Background technique [0002] Hyaluronic acid is a linear anionic mucopolysaccharide composed of glucuronic acid and N-acetylglucosamine linked by β-1,3 and β-1,4 glycosidic bonds. It was obtained from the bovine vitreous by Meyer and Palmer in 1934. seperate. Hyaluronic acid is an important component in extracellular matrix, synovial fluid, cartilage tissue, and umbilical cord, and has important physiological functions in the human body, such as regulating the chemical composition of extracellular fluid, lubricating and promoting wound healing; regulating adhesion, growth, Movement and activation of specific cells, etc. [0003] The molecular weight of hyaluronic acid is closely related to its physiological function. For...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/46
Inventor 陈坚堵国成王淼刘龙
Owner JIANGNAN UNIV
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