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Combined degerming method for L-lactic fermentation liquid

A technology of lactic acid fermentation and phase liquid, which is applied in the field of sterilization and combined sterilization of L-lactic acid fermentation liquid. It can solve the problems of slow filtration speed, low dryness of bacteria sludge, and large amount of flocculant added, so as to improve filtration speed, The effect of improving the purity of the clear liquid and remarkable economic benefits

Inactive Publication Date: 2008-11-19
ZHANGJIAGANG SANYUAN BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are following defects: 1. the addition of flocculant is relatively large; 2. its process belongs to full filtration, and the filtration speed is slow; 3. the purity of the filtrate is poor, and the light transmittance is below 40%; 4. without sufficient washing, the product yield is low Low, the yield is less than 96%
[0004] At present, the sterilization and purification process of L-lactic acid fermentation broth is relatively simple, the purity of the filtrate is low, the product yield is not high, the dryness of the bacteria sludge is small, and the production capacity is low.

Method used

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  • Combined degerming method for L-lactic fermentation liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Select 26L of L-lactic acid fermentation broth, L-lactic acid content 16.5% (mass volume percentage), bacterium content 1.2% (mass volume percentage), residual sugar content 0.1%.

[0027] First, heat the fermentation broth to 85°C, add Ca(OH) 2 Adjust the pH to 11, keep stirring for 20 minutes. The obtained feed liquid was separated by a disc centrifuge with a separation factor of 6000g to obtain a dense phase liquid and a clear phase liquid.

[0028] Heat the obtained dense phase liquid (about 5% of the bacteria) to 86°C, filter with a plate and frame filter press, keep the initial pressure at 0.04MPa, and maintain the pressure at the later stage at 0.10MPa, wash with hot water at 80°C after the filter press , when the lactic acid content in the lotion is ≤0.1%, stop washing. Dry it with compressed air, and keep the pressure at about 0.1MPa. Filter cake water content is about 60% (mass percentage), contains lactic acid and is about 0.1% (mass percentage); Simultane...

Embodiment 2

[0032] Select L-lactic acid fermentation broth 48m 3 , wherein the L-lactic acid content is 16%, the cell content is 1.5%, and the residual sugar is 0.12%.

[0033] First, heat the fermentation broth to 90°C, add Ca(OH) 2 Adjust the pH to 12, keep stirring for 30 minutes. The obtained feed liquid was separated by two FESX-412 type disc centrifuges with a separation factor of 8000g to obtain a dense phase liquid and a clear phase liquid.

[0034] Will get 9.6m 3 The dense phase liquid (about 7.5% of bacteria) was heated to 98°C, and five plate and frame filter presses were used for pressure filtration. The initial pressure was maintained at 0.06MPa, and the later pressure was maintained at 0.12MPa. After the pressure filtration was completed, it was washed with hot water at 90°C. When the lactic acid content in the lotion is ≤0.1%, stop washing. Dry it with compressed air and keep the pressure at about 0.2MPa. Filter cake moisture content is about 55% (mass percentage), an...

Embodiment 3

[0038] Select 30L of L-lactic acid fermentation broth, L-lactic acid content 17% (mass volume percentage), bacterium content 1.3% (mass volume percentage), residual sugar content 0.08%.

[0039] First, heat the fermentation broth to 80°C, add Ca(OH) 2 Adjust the pH to 10 and keep stirring for 10 minutes. The obtained feed liquid was separated by a disc centrifuge with a separation factor of 4000g to obtain a dense phase liquid and a clear phase liquid.

[0040]Heat the obtained dense phase liquid (about 5% of the bacteria) to 75°C, filter with a plate and frame filter press, keep the initial pressure at 0.03MPa, and keep the pressure at 0.08MPa in the later stage, and wash with hot water at 70°C after the filter press is completed , when the lactic acid content in the lotion is ≤0.1%, stop washing. Dry it with compressed air and keep the pressure at about 0.3MPa. Filter cake water content is about 65% (mass percentage), contains lactic acid and is about 0.1% (mass percentag...

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Abstract

The invention relates to a method for L-lactic acid fermentation broth composite degermation. The method adopts the following process that: firstly, fermentation broth is heated and then CaOH2 is added with the pH value of the solution adjusted to 10 to 12; the feed liquid undergoes thalli separation through a disk centrifuge so as to obtain concentrated phase liquid and clear-phase liquid; the obtained concentrated phase liquid is heated and then undergoes pressure filtration through a plate-and-frame filter press; after pressure filtration is completed, the concentrated phase liquid is cleaned by hot water, and the content of the lactic acid in the washing liquid is controlled to less than or equal to 0.1 percent; the washing liquid undergoes dry-pressing by compressed air, and the obtained washing liquid is added in the clear-phase liquid; the clear-phase liquid is heated and then coagulant aid and flocculant are added in the clear-phase liquid so as to carry out heat-preservation stirring; after more than 1 hour of heat-preservation deposition, filtrate is obtained through sucking filtration; No. 302 powder active carbon is added in the filtrate to carry out heat-preservation stirring, and then filtrate is obtained through sucking filtration; finally, the obtained filtrate undergoes secondary filtration and then is blown dry when cleaned by hot water. The technical proposal can obtain more than or equal to 99.5 percent of yield and extremely high quality of clear liquid, and greatly reduce solid content; moreover, the technical proposal has strong production treatment capacity, low energy consumption and enormous application prospect.

Description

technical field [0001] The invention relates to a method for removing bacteria, in particular to a method for combining bacteria removing with L-lactic acid fermentation broth, and belongs to the technical field of biochemical industry. Background technique [0002] As we all know, the sterilization of fermentation broth is very important in the production process of L-lactic acid, which is closely related to the product quality. The sterilization process has a great impact on the yield and energy consumption of the product. [0003] Prior art, L-lactic acid fermentation broth sterilization and purification process: first neutralize and heat the L-lactic acid fermentation broth, and add CaO to adjust the pH; then add a large amount of flocculant to the feed liquid, and use a plate and frame filter press after flocculation Press filter, wash, or centrifuge with a centrifuge after flocculation to obtain a supernatant. There are following defects: 1. the addition of flocculant...

Claims

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Application Information

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IPC IPC(8): C12P7/56
Inventor 沈京富蔡德龄
Owner ZHANGJIAGANG SANYUAN BIOLOGICAL ENG