Skin care cosmetic and skin and agent for preventing skin roughness containing biosurfactants
A skin care and biological surface technology, applied in the field of skin roughness improving agent, cosmetics, skin care/skin roughness improving cosmetics, biosurfactant or its premix, to achieve the effect of improving skin roughness and easy production
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Embodiment 1
[0084] Embodiment 1: the preparation of MEL
[0085] One loop (loop) of Pseudozyma antarctica NBRC 10736 was inoculated in a seed medium (20 ml / 500 ml Sakaguchi flask) for inoculum culture. Overnight incubation was performed at 30°C. The obtained medium was used as an inoculum. The seed medium consisted of the following: 4% glucose, 0.3% NaNO 3 , 0.02% MgSO 4 ·H 2 O, 0.02% KH 2 PO 4 and 0.1% yeast extract. The above inoculum (75 ml) was inoculated in 1.5L (5L-tank) production medium and cultured at 30°C, 300rpm (stirring frequency) and 0.5L / min0 (air) (using 5L-tank). The production medium consists of 3% soybean oil, 0.02% MgSO 4 ·H 2 O, 0.02% KH 2 PO 4 and 0.1% yeast extract. The medium (250 ml) was centrifuged (6,500 rpm, 30 minutes), the supernatant was removed, and the precipitate (cells) was collected. Ethyl acetate (50 mL) was added to the precipitate, which was then thoroughly stirred and centrifuged (8,500 rpm, 30 minutes) to separate the supernatant from ...
Embodiment 1A
[0086] Example 1A: Preparation of MEL-B
[0087] In a 500-ml Sakaguchi flask, frozen-stored P. tsukubaensis (0.2 ml) was inoculated in 20 ml of YM seed medium and cultured overnight at 26°C and 180 rpm to prepare seed inoculum. In a 500 ml Sakaguchi flask, the seed inoculum was inoculated again in 20 ml of YM seed medium and cultured overnight at 26°C, 180 rpm, as an inoculum. Inoculum (20 ml) was inoculated in 2 L of YM medium in a 5 L tank and cultured at 26° C. at 300 rpm (1 / 4 VVM, 0.5 L air / min) for 8 days. The medium was centrifuged at 7,900 rpm at 4° C. for 60 minutes to separate bacterial cells (including MEL-B) from the supernatant. Ethyl acetate (80 ml) was added to the cell fraction, which was then shaken until thoroughly suspended, followed by centrifugation at 7,900 rpm at 4°C for 30 minutes. An equal amount of brine was added to the obtained supernatant, and the mixture was stirred to yield an ethyl acetate layer. An appropriate amount of anhydrous sodium sulfa...
Embodiment 2
[0089] Although soybean oil is used as a raw material in the preparation of MEL in Example 1, olive oil is used instead as a raw material for production in this example to separate and purify MEL-A, MEL-B and MEL-C, and use and Example 1 Cultivate in the same way. The MEL fractions obtained at this time were referred to as MEL-A(OL), MEL-B(OL) and MEL-C(OL) in order to distinguish them from the MEL obtained in Example 1.
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