Method for extracting betulin from birch bark

A technology of betulin and birch bark, which is applied in the fields of phytochemistry and food chemistry, can solve the problems of multiple reflux extractions, cumbersome extraction steps, complicated purification operations, etc., and achieve shortened extraction time, high biological activity of products, and efficient extraction effect of effect

Inactive Publication Date: 2008-12-24
ZHEJIANG UNIV
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Problems solved by technology

Although the purity of the obtained product can reach more than 90%, the number of times of reflux extraction is more, and the purification operation is more complicated
[0006] The methods reported so far are mainly based on the traditional solvent extraction method (ie, reflux method), the extraction steps are cumbersome, the loss is large, the chemical residue is high, and the safety is not high

Method used

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  • Method for extracting betulin from birch bark

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Grind the dried birch bark with a pulverizer, weigh 2.0g of the bark powder and place it in a 250ml round-bottomed flask; add 50ml of 80% ethanol solution, ultrasonically reflux for 3 hours, the ultrasonic temperature is 60°C, and the ultrasonic power is 550W; reflux extraction After the end, the residue was removed by filtration and separation to obtain a brown-yellow extract; the filtrate was distilled under reduced pressure on a rotary evaporator, and a yellow solid powder was precipitated; dried at 60°C to a constant weight to obtain 0.596 g of a crude product of betulin, which was obtained by reverse-phase high-efficiency Liquid chromatography (RP-HPLC) analysis showed that the purity of the crude product of betulin was 81.101%, and the extraction yield was 24.160%.

Embodiment 2

[0030] Grind the dried birch bark with a pulverizer, weigh 2.0g of the bark powder and place it in a 250ml round bottom flask; add 80ml of 62% ethanol solution, ultrasonically reflux for 1 hour, ultrasonic temperature is 40°C, ultrasonic power is 220W; reflux extraction After the end, filter and separate to remove the residue to obtain a brown-yellow extract; distill the filtrate under reduced pressure on a rotary evaporator to precipitate a yellow solid powder; dry at 60°C to a constant weight to obtain 0.300 g of the crude product of betulin, which was obtained by reverse-phase high-efficiency Liquid chromatography (RP-HPLC) analysis showed that the purity of the crude product of betulin was 63.831%, and the extraction yield was 9.57%.

Embodiment 3

[0032] Grind the dried birch bark with a pulverizer, weigh 2.0g of the bark powder and place it in a 250ml round bottom flask; add 50ml of 99.74% ethanol solution, ultrasonically reflux for 6 hours, ultrasonic temperature is 60°C, ultrasonic power is 550W; reflux extraction After the end, filter and separate to remove the residue to obtain a brown-yellow extract; distill the filtrate under reduced pressure on a rotary evaporator to precipitate a yellow solid powder; dry at 60°C to a constant weight to obtain 0.700 g of the crude product of betulin, which was obtained by reverse-phase high-efficiency Liquid chromatography (RP-HPLC) analysis showed that the purity of the crude product of betulin was 73.132%, and the extraction yield was 25.596%.

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Abstract

The invention discloses a method for extracting betulin from birch barks, comprising the following steps: firstly, crushed birch barks are added into an ethanol water solution, undergo reflux extraction under auxiliary processing of ultrasonic waves, and then are filtrated for removal of residue, and an extract is obtained; secondly, solid powder is separated out after decompression and concentration of the extract, and then dried to the constant weight so as to obtain betulin coarse products; and thirdly, the betulin coarse products are added into an organic solvent, undergo ultrasonic treatment and then stand, crystals are separated out, and the betulin is obtained after the separated crystals are dried to the constant weight. The method has a simple operation flow, short extraction time, low production cost and high safety, and provides a better method and a better platform for further development and utilization of natural products.

Description

technical field [0001] The invention relates to the fields of phytochemistry and food chemistry, in particular to a method for efficiently obtaining betulin from birch bark by using ultrasonic extraction combined with a reflux method. Background technique [0002] White birch (Betula platyphylla Suk) is a plant of the genus Betula in the Betaceae family. It is a cold-resistant deciduous tree with a height of about 15 meters. Birch bark and birch sap have the effects of clearing away heat and dampness, relieving cough and eliminating phlegm. In recent years, the pharmacological effects of birch bark have been widely valued by scholars at home and abroad. The chemical composition of birch bark is complex and the pharmacological effects are diverse. Its extracts have antibacterial, antiviral, antitumor, lipid-lowering, choleretic and hepatoprotective effects. . Extensive research has been done abroad on the chemical composition and utilization of bark such as Betula verruca a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 陈启和刘婧何国庆傅明亮章海锋阮晖
Owner ZHEJIANG UNIV
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