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Preparation method of acetic acid inducted rat individual same-size gastric ulcer model

A gastric ulcer, single technology, applied in the field of anti-gastric ulcer drug evaluation, can solve the problems of difficult to solve adhesion problems, difficult to control the area and depth of ulcers, etc.

Inactive Publication Date: 2008-12-31
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, a significant problem of this modeling method is that the ulcer area and depth are not easy to control, and the adhesion problem after the modeling operation is not easy to solve

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take 128 rats, fasting and drinking water freely for 25 hours. Rats were anesthetized by intramuscular administration of 10% chloral hydrate (0.3 ml / 100 g body weight). Disinfect the abdominal skin of the rat with povidone iodine, make an incision on the ventral side wall, and pull out the stomach gently. Clamp the gastric body area 3mm away from the pylorus with forceps (inner diameter 9mm) with a circular ring at the front end. After penetrating the distal gastric antrum, 0.18 ml of mineral oil and 0.02 ml of 60% acetic acid solution (total volume 0.2 ml) were sequentially injected into the clamped lumen. After 40 seconds, suck out the mixed solution of acetic acid and mineral oil from the stomach, then inject 2ml of normal saline into the stomach cavity for flushing, suck it out after 1 min, and finally return the stomach and suture the abdomen. Animals were routinely cared for postoperatively. The results were 96% molded, and the efficacy and mechanism of anti-ga...

Embodiment 2

[0011] Take 140 rats, fasting and drinking water freely for 24 hours. Rats were anesthetized by intramuscular administration of 10% chloral hydrate (0.3 ml / 100 g body weight). Disinfect the abdominal skin of the rat with povidone iodine, make an incision on the ventral side wall, and pull out the stomach gently. Clamp the gastric body area 3mm away from the pylorus with forceps (inner diameter 9mm) with a circular ring at the front end. After penetrating the distal gastric antrum, 0.18 ml of mineral oil and 0.02 ml of 60% acetic acid solution (total volume 0.2 ml) were sequentially injected into the clamped lumen. After 45 seconds, the mixed solution of acetic acid and mineral oil was sucked away from the stomach, and then 2ml of normal saline was injected into the stomach cavity for flushing, sucked out after 1 min, and finally the stomach was returned and the abdomen was sutured. Animals were routinely cared for postoperatively. The results were 98% molded, and the curati...

Embodiment 3

[0013] Take 160 rats, fasting and drinking water freely for 26 hours. Rats were anesthetized by intramuscular administration of 10% chloral hydrate (0.3 ml / 100 g body weight). Disinfect the abdominal skin of the rat with povidone iodine, make an incision on the ventral side wall, and pull out the stomach gently. Clamp the gastric body area 3mm away from the pylorus with forceps (inner diameter 9mm) with a circular ring at the front end. After penetrating the distal gastric antrum, 0.18 ml of mineral oil and 0.02 ml of 60% acetic acid solution (total volume 0.2 ml) were sequentially injected into the clamped lumen. After 50 seconds, the mixed solution of acetic acid and mineral oil was sucked away from the stomach, and then 2ml of normal saline was injected into the stomach cavity for flushing, sucked out after 1 min, and finally the stomach was returned and the abdomen was sutured. Animals were routinely cared for postoperatively. The results were 100% modeled, and the cura...

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Abstract

The invention relates to a manufacturing method of a single and isometric gastric ulcer model induced by acetic acid in a rat. The method comprises procedures as follows: the rat is fasting but drinks freely for 24 hours; the chloral hydrate of 10 percent (0.3nl / 100g weight) is used for anesthetizing the rat by muscle administration; lodophor is used for sterilizing the abdomen skin of the rat; a cut is made on the side wall of the abdomen and a stomach is pulled out slightly; the nipper with an annular front end (with the inner diameter of 9mm) clamps a corpus ventriculi part 3mm away from a pyloric part; after a distal stomach-antrum is penetrated, 0.81ml of the mineral oil and the acetic acid solution of 160 percent of 0.02ml ( with the total volume of 0.2ml) are injected into a lumen; after 45 seconds, the mixed solution of acetic acid and the mineral oil is absorbed from stomach; afterwards, the physiological saline of 2ml is injected into a gastral cavity for flushing and is drained off after 1min; and at last, the stomach is put back and the abdomen is sewed up. After an operation, the rat is carried out usual care. The method is easy to be handled and can make a single round ulcer with the inner diameter of 9mm. The area and the depth of the ulcer can be controlled. In addition, the problem of adhesion can be solved; the ulcer is extremely similar with the human ulcer in pathological features and healing mechanism; and the ulcer model is very sensitive to various anti-ulcer drugs.

Description

technical field [0001] The invention relates to a method for preparing an experimental animal model of a disease (rat gastric ulcer), which can be used for research on the mechanism of gastric ulcer and evaluation of anti-gastric ulcer drugs. Background technique [0002] The gastric ulcer model in rats induced by injection of acetic acid solution into the gastric mucosa was established in 1969, and its importance in the scientific community is obvious to all—in the past ten years, there have been about 35 to 30 research papers published each year using this model. 40 articles. The reasons for its wide application are listed in the following items: 1) The operation process of ulcer induction is simple, and the incidence rate is 100%. 2) The ulcer model is highly similar to human ulcers in terms of pathological characteristics and healing mechanism, and the spontaneous recurrence of healed ulcers is just like human peptic ulcers. 3) The ulcer model is highly sensitive to va...

Claims

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Application Information

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IPC IPC(8): A61B19/00A61K49/00A61D1/00
Inventor 才丽平林蔗茹蒋宁曲怡夏淑杰郑洪新
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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