Device and method for capturing image of a sample originating from organism

A sample image and biological source technology, applied in material excitation analysis, fluorescence/phosphorescence, instruments, etc., can solve problems that are difficult to analyze in detail, and achieve the effect of reducing failures
CN101351735AInactive Publication Date: 2009-01-21OLYMPUS CORP
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
OLYMPUS CORP
Publication Date
2009-01-21
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

A novel apparatus and a novel method of imaging a luminescence phenomenon of a sample of biological origin using an optical image formation device are provided. In the inventive method and apparatus, an illumination image acquired by illuminating the sample and a luminescence image acquired with the light emitted by a luminescence phenomenon of a cell in the sample without illuminating it are superposed to generate a superposition image. In the superposition image, an analysis region is determined. Thereby, even when the light detected in a luminescence observation is feeble, a luminescent cell and / or a luminescent site in a cell in a sample can be specified.
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Description

technical field

[0001] The present invention relates to a method and device for imaging low light emitted by biological source samples (or specimens) such as cells, bacteria and other biological samples, more specifically, the present invention relates to low light imaging by bioluminescent phenomena And methods and devices for observing and various measurements of such samples. Background technique

[0002] In recent years, techniques for imaging samples of biological origin, such as cells, with fluorescent proteins such as GFP, and / or luminescent proteins, such as luciferase, luminescent proteins, have been increasingly used in research in the field of life sciences. Expression in any cell by transgenesis. Fluorescent or luminescent proteins can be expressed while mixed with another arbitrary protein (protein marker) in the cell, and will only be based on specific genetic regulation if a fluorescent or luminescent protein-encoding gene is inserted or replaced downstream o...

Claims

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