Method for rapidly detecting malachite green in aquatic products by Aptamer

Abstract

The present invention discloses a method which adopts the aptamer rapidly to detect the malachite green in water products. The sample to be detected, the malachite green aptamer and the report sequence with detection markers are mixed; after the report sequence and the malachite green are competitively combined with the malachite green aptamer, the markers on the report sequence is detected, and the content of the malachite green in the sample to be detected is calculated. The kit and the fast detecting test paper, which are prepared according to the method, can better satisfy the current urgent needs for detecting the malachite green residue in the water products, can be used in the detection of the customs and the food sanitation department, and can be easily promoted and applied in a large scale.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a method for detecting malachite green in aquatic products. Background technique [0002] Malachite green (Malachite green, referred to as MG), belongs to triphenylmethane dyes, and its metabolite is colorless malachite green (Leucomalachite green, LMG). For a long time, because malachite green has good antifungal properties in aquaculture disease, freshness of aquatic products and low price, etc. Therefore, it has become the "fishing medicine" favored by many farmers to use, but these two substances have side effects such as high toxin, high residue, high teratogenicity and mutagenicity, not only It has brought huge obstacles to the export of aquatic products in our country, and more importantly, it has constituted a potential threat to people's health. [0003] In view of the harmfulness of malachite green and its metabolites, many countries have listed it as a banned drug for aq...

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Problems solved by technology

[0003] In view of the harmfulness of malachite green and its metabolites, many countries have listed it as a banned drug for aquaculture, and my country also listed it in the "List of Veterinary Drugs and Their Compounds Banned for Food Animals" in May 2002. However, Due to the weak awareness of aquaculture farmers' healthy farming and the imperfect safety management mechanism of aquatic products, in 2005 alone, various aquatic products (such as: eel, eel, High residues of malachite green were detected in soft-shelled turtles, etc.

[0004] At present, there are mainly three kinds of detection methods about malachite green and its metabolites, but these methods all have certain limitations: (1) high performance liquid chromatography, when the content is low, it is difficult to qualitatively utilize the retention time; 2) GC method can only be used to qualitatively colorless malachite green; (3) liquid mass spectrometry (LC / MS) has better effect but is expensive; therefore, malachite green and its metabolites The establishment of fast, stable and reliable residue detection methods in aquatic animals has become a technical problem to be solved urgently at home and abroad

[0008] However, it is very difficult to obtain high-efficiency monoclonal antibodies in this technology, which also increases the cost

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