Method for curing soil by using carbonate mineralized bacterium

A technology of mineralizing bacteria and carbonate, applied in the field of solidifying soil with carbonate mineralizing bacteria

Active Publication Date: 2009-02-18
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Currently the technology is still in the exper...

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  • Method for curing soil by using carbonate mineralized bacterium
  • Method for curing soil by using carbonate mineralized bacterium
  • Method for curing soil by using carbonate mineralized bacterium

Examples

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preparation example Construction

[0020] Preparation of highly concentrated bacterial liquid:

[0021] Inoculate the strain Bacillus pasteurii into the beef extract peptone culture solution, each liter of the culture solution contains 4-6g of peptone and 2-4g of beef extract, and control the pH to 6-8, culture at 30°C for 16-24h, Take out and centrifuge at 5000-8000rpm for 5-8min to get the lower layer to obtain highly concentrated bacterial liquid, the strain concentration is 2×10 9 ~2×10 11 cell / mL.

Embodiment 1

[0023] Mix the soil with lime that occupies 3% to 7% of the mass of the soil, and the strain concentration is 2×10 11 Cell / mL of highly concentrated bacterial liquid 6mL, urea 4.8g, water, and supernatant were combined separately, wherein the supernatant was the supernatant obtained by centrifugation when preparing the highly concentrated bacterial liquid, and the test was carried out instead of water, as shown in Table 2. After uniform mixing, it is compacted, demoulded and cured in a constant temperature room at 20°C. Three specimens are molded for each group, and the compressive strength is tested after 7 days, as shown in Table 3.

[0024] Table 2 Scheme design of soil stabilization by microbial technology

[0025]

[0026] Table 3 7-day compressive strength of soil specimens in each group

[0027]

[0028] According to the test, the highest 7-day compressive strength is the T6 (soil + bacteria + urea solution) group, and the lowest strength is th...

Embodiment 2

[0030] For the formula of the T12 group, another group of test pieces T12' was used as a control: the concentration of the strain was 2×10 11 Add 6mL of cell / mL high-concentration bacterial solution into the dry soil, and add 50mL of freshly prepared culture solution, each liter of the culture solution contains 5g of peptone and 3g of beef extract, let it stand for 4 days and 8 days respectively, and then mix it with dry soil 5.2g of lime with 3% mass and 4.8g of urea were molded, and the strength of the two groups of specimens was tested as shown in Table 4.

[0031] Table 4 7-day compressive strength of two groups of specimens

[0032]

[0033] According to the formula of T12', molded according to the same method, the compressive strength measured 4 days and 8 days respectively reached 1.22, 1.16MPa, which is about 27% higher than that of the direct molding in the T12 group, and about 70% higher than that of the original plain soil. %. This is because the ac...

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Abstract

The invention discloses a soil curing method through carbonate-mineralization microbes, including the following steps: first, preparing high concentration microbe liquid: inoculating Bacillus pasteurii strain into the culture fluid of beef extract and peptone; wherein each litre of culture fluid contains 4-6g peptone and 2-4g beef extract; cultivating for16-24h under30DEG C with the pH value controlled at 6-8, then getting out the mixture and centrifuging for 5-8min under the speed of 5000-8000rpm, thus getting high-concentration microbe liquid at concentration of 2 X 10<9>-2 X 10<11>cell/ml; second, mixing the soil: mixing the high-concentration microbe liquid prepared in the first step and the newly prepared culture fluid into the dried soil sample through lime(3%-7% the weight of the dry soil) and carbamide(2%-6% the weight of the dry soil) according the proportioning of 100g dry soil per 2-3mL high-concentration microbe liquid and 0-50mL newly prepared culture fluid, blending to be even and then cultivating for 0.5-8days; third; molding through a die: molding the soil mixture cultivated in second step in a die when the mixture reaches the optimal water ratio, then demoulding and conserving in constant temperature of 20 DEG C. Seven days later, the soil will be cured, with the compressive resistance reaching above 1.0MPa, up by 38% as compared with pure soil.

Description

technical field [0001] The invention relates to a method for solidifying soil by utilizing carbonate mineralizing bacteria, which mainly utilizes the fiber filling function and biomineralization function of microorganisms to mineralize and consolidate soil. Background technique [0002] Some microorganisms in nature can undergo enzymatic action with the surrounding environmental medium through their own life activities, and gradually mineralize to form calcite. After a long period of accumulation, the loose clastic materials deposited in nature are finally cemented into hard rocks. [0003] At present, this technology has been widely studied and applied in the repair of defects in cement-based materials, the restoration of the original appearance of architectural relics, the consolidation of heavy metal ions in soil, and the solidification of deserts. At present, this technology is still in the experimental research stage in terms of soil stabilization. Contents of the in...

Claims

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Application Information

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IPC IPC(8): E02D3/12
Inventor 钱春香陆旺杰王瑞兴王剑云
Owner SOUTHEAST UNIV
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