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Method for controlling micrometre zinc sulphide morphology by Escherichia coli biological template

A technology of Escherichia coli and biological templates, applied in the direction of zinc sulfide, etc., can solve the problems of difficult control of particle shape and size, and achieve the effect of improving shape and stability, improving conversion rate, and great practical significance

Inactive Publication Date: 2009-02-25
YANSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the problem that the particle shape and size of existing methods for preparing zinc sulfide are difficult to control, the present invention provides a method for controlling the morphology of micron-sized zinc sulfide by using Escherichia coli biological templates

Method used

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  • Method for controlling micrometre zinc sulphide morphology by Escherichia coli biological template
  • Method for controlling micrometre zinc sulphide morphology by Escherichia coli biological template
  • Method for controlling micrometre zinc sulphide morphology by Escherichia coli biological template

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Prepare 50 ml of LB liquid medium, introduce Escherichia coli strains, and culture on a shaker at 200 r / min at 37°C for 12 hours. Then place the E. coli culture solution on ice for 10 minutes, centrifuge at 6°C, 3000r / min for 15min, discard the supernatant, dissolve the precipitate in 40ml of deionized water, and centrifuge at 6°C, 3000r / min for 15min for hypotonicity. Repeat once and take the pellet. The precipitate was dissolved in 40ml, 0.01mol / L of Zn(CH 3 COO) 2 2H 2 O solution (pre-cooled in a refrigerator at 4°C), mix well, let stand on ice for 5 minutes, centrifuge at 6°C, 3000r / min for 15min, and collect the precipitate. Redissolve the precipitate in 40ml of pre-cooled 0.01mol / L Zn(CH 3 COO) 2 2H 2 O solution, mixed thoroughly to obtain competent cells. Weigh 0.091g of CS(NH 2 ) 2 Add it to the competent cell suspension, place it on ice for 10 minutes, let it stand in a refrigerator at 4°C for 20 hours, heat shock it in a hot water bath at 35°C for 1 m...

Embodiment 2

[0022] Prepare 50ml of LB liquid medium, introduce Escherichia coli strains, and culture on a shaker at 150r / min at 37°C for 17h. Then place the E. coli culture solution on ice for 10 minutes, centrifuge at 4°C, 4000r / min for 10min, discard the supernatant, dissolve the precipitate in 40ml of deionized water, and centrifuge at 4°C, 4000r / min for 10min for hypotonicity. Repeat twice and take the pellet. The precipitate was dissolved in 40ml, 0.1mol / L of Zn(CH 3 COO) 2 2H 2 O solution (pre-cooled in a refrigerator at 4°C), mix well, let stand on ice for 10 minutes, centrifuge at 4°C, 4000r / min for 10 minutes, and collect the precipitate. Redissolve the precipitate in 40ml pre-cooled 0.1mol / L Zn(CH 3 COO) 2 2H 2 O solution, mixed thoroughly to obtain competent cells. Weigh 0.912g of CS(NH 2 ) 2Add it to the competent cell suspension, place it on ice for 20 minutes, let it stand in a refrigerator at 4°C for 40 hours, heat shock it in a hot water bath at 42°C for 3 minutes...

Embodiment 3

[0024] Prepare 50ml of LB liquid medium, introduce Escherichia coli strains, and culture on a shaker at 120r / min at 37°C for 20h. Then place the E. coli culture solution on ice for 20 minutes, centrifuge at 0°C, 5000r / min for 8min, discard the supernatant, dissolve the precipitate in 40ml of deionized water, and centrifuge at 0°C, 5000r / min for 8min for hypotonicity. Repeat twice and take the pellet. The precipitate was dissolved in 40ml, 0.1mol / L of Zn(CH 3 COO) 2 2H 2 O solution (pre-cooled in a refrigerator at 4°C), mix well, let stand on ice for 15 minutes, centrifuge at 0°C, 5000r / min for 8 minutes, and collect the precipitate. Redissolve the precipitate in 40ml pre-cooled 0.1mol / L Zn(CH 3 COO) 2 2H 2 O solution, mixed thoroughly to obtain competent cells. Weigh 0.912g of CS(NH 2 ) 2 Add it to the competent cell suspension, place it on ice for 30 minutes, let it stand in a refrigerator at 4°C for 100 hours, heat shock it in a hot water bath at 45°C for 5 minutes,...

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Abstract

The invention relates to a method for controlling micron zinc sulfide shape by an escherichia coli biological template. A micron zinc sulfide material with excellent shape and a short rod-shaped structure is obtained by utilizing the space confinement effect of the biological template, taking the escherichia coli as the biological template, preparing competent cells which incubate with the zinc sulfide system and combine with thermal shock and calcination treatment. The shape control of the zinc sulfide material is carried out by the biological confinement effect existing in nature to obtain the zinc sulfide material with uniform shape and same size, which proves that the biological template can accurately regulate and control the synthesis of the material. The method has the advantages of simple process, mild conditions, available materials, low cost and that the obtained material has excellent shape characteristics. In addition, as the biological template has the characteristics of self-breeding and high shape repeatability, and the like, the method can easily realize large-scale production.

Description

technical field [0001] The invention relates to the technical field of preparation of inorganic materials, in particular to a method for controlling the morphology of micron-sized zinc sulfide by using Escherichia coli biological templates. Background technique [0002] Metal sulfides have excellent electrical properties and are widely used in semiconductors, pigments, photoluminescent devices, solar cells, infrared detectors, optical fiber communications, etc. Among them, zinc sulfide is an important luminescent material and semiconductor material. It has high infrared transmittance and excellent optical, mechanical and thermal performance in the 3-5μm and 8-12μm bands. It is mainly used in the electronics industry and defense industry. It is one of the widely researched and applied materials in II-VI compounds. Zinc sulfide is also a fluorescent matrix material with unique properties, which has been widely used in various instruments and meters, such as flat panel display...

Claims

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Application Information

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IPC IPC(8): C01G9/08
Inventor 高发明侯莉李娜赵光萍
Owner YANSHAN UNIV
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