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Intra-polysaccharides from mycelia of ganoderma sinensis, and preparation and use thereof

An intracellular polysaccharide and mycelium technology, applied in fungi, pharmaceutical formulations, medical preparations containing active ingredients, etc., can solve problems such as not much research

Inactive Publication Date: 2013-06-19
SHANGHAI INST OF PHARMA IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many reported studies at present, among which Zhang Weiguo et al. (Zhang Weiguo, Zhao Yuntao, Liu Xin. Distribution of molecular weight polysaccharides in solid fermentation 9519 Zizhi mycelia; Journal of Shaoguan University, 2003; 24(6): 70-73) reported Molecular weight distribution of polysaccharides from the solid-fermented mycelia of purple ganoderma; the Chinese patent with publication number CN1436842A (publication date: August 20, 2003) discloses a high-yield polysaccharide and its fermentation production process, mainly using a semi-solid fermentation method

Method used

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  • Intra-polysaccharides from mycelia of ganoderma sinensis, and preparation and use thereof
  • Intra-polysaccharides from mycelia of ganoderma sinensis, and preparation and use thereof
  • Intra-polysaccharides from mycelia of ganoderma sinensis, and preparation and use thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The preparation of embodiment 1 Zizhi mycelia

[0024] The Zizhi strain CGMCC5.69 was transferred to a PDA agar slant (containing 35% potato, 2.5% glucose, 0.3% malt extract and 1.7% agar, and the balance was water), and cultured at 25°C for 6 days to obtain a seed slant for fermentation research; Transfer to 3 750ml shake flasks with 100ml seed culture medium; The seed culture medium consists of: bean cake powder 2%, glucose 2.5%, anhydrous magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, and the balance is water, The pH is natural; cultivated at 25°C and 220rpm for 4 days, according to the inoculum size of 10v / v%, 100ml seed solution was transferred to 10 750ml shake flasks with 100ml fermentation medium (fermentation medium composition: 4% sucrose, peptone 0.4%, yeast extract 0.2%, anhydrous magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, the balance is water, pH6.0), 28 ℃, 190rpm shaker culture for 5 days. After the fermentation is over, ...

Embodiment 2

[0025] The preparation of embodiment 2 Zizhi mycelium

[0026] Inoculate Zizhi strain ACCC50045 on PDA slant medium (containing 30% potato, 3% glucose, 0.5% malt extract and 1.4% agar), culture at 25°C for 7 days, transfer to 4 seeds containing 100ml of medium to obtain 750ml Triangular shake flask (seed medium: 1% bean cake powder, 5% glucose, 0.30% anhydrous magnesium sulfate, 0.1% potassium dihydrogen phosphate, natural pH), 28°C, 200rpm for 6 days, according to 5v / v% inoculum Transfer to ten 750ml Erlenmeyer flasks with 100ml fermentation medium, culture at 25°C and 220rpm for 7 days, fermentation medium: 2.5% sucrose, 0.6% peptone, 0.1% yeast extract, 0.10% anhydrous magnesium sulfate, phosphoric acid Potassium dihydrogen 0.5%, pH6.5. Mycelium separation is the same as in Example 1; the weight is 0.23g / ml fermentation broth.

Embodiment 3

[0027] The preparation of embodiment 3 Zizhi mycelium

[0028] Inoculate Zizhi strain ACCC50468 on PDA slant medium (containing 40% potato, 2% glucose, 0.2% malt extract and 2.0% agar), culture at 25°C for 7 days, transfer to 4 seeds containing 100ml of medium to obtain 750ml Triangular shake flask (seed medium: 3% bean cake powder, 1.5% glucose, 0.30% anhydrous magnesium sulfate, 0.4% potassium dihydrogen phosphate, natural pH), 26°C, 200rpm for 4 days, according to 15v / v% inoculum size Transfer to ten 750ml Erlenmeyer flasks with 100ml fermentation medium, culture at 26°C and 220rpm for 4 days, fermentation medium: 6% sucrose, 0.2% peptone, 0.3% yeast extract, 0.3% anhydrous magnesium sulfate, phosphoric acid Potassium dihydrogen 0.2%, pH5.5. Mycelium separation is the same as in Example 1; the weight is 0.18g / ml fermentation broth.

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Abstract

The invention discloses amylase in a G.japonicum mycelium cell, and a preparation method thereof. The preparation method comprises the following steps: the G.japonicum mycelium obtained by fermentation of Gjaponicum is extracted through a conventional amylase extraction method, wherein, the method for fermenting the G.japonicum comprises the slant culture of the G.japonicum strains one by one, primary seed culture and the submerged liquid fermentation of fermentation culture. In the invention, the parameters and the culture medium of the G.japonicum submerged fermentation process are improved, so that the content of the amylase extracted from the obtained G.japonicum mycelium cell reaches 39 percent to 75 percent, and the amylase has quite strong anti-tumor activity.

Description

technical field [0001] The present invention relates to a kind of intracellular polysaccharide of Ganoderma lucidum, in particular to a kind of intracellular polysaccharide of Ganoderma lucidum mycelia prepared by adopting the Zizhi fermentation method of submerged liquid fermentation and its preparation method and application. tumor activity. Background technique [0002] Ganoderma lucidum [Gamoderma lucidum (Leys ex Fr.) Karst] is a Basidiomycetes, Polyporaceae, Ganoderma fungus. Ganoderma lucidum is sweet in taste, warm in nature, and non-toxic. It can replenish the qi of the heart, liver, spleen, lung, and kidney. The active ingredients are mainly polysaccharides (namely: Ganoderma lucidum polysaccharide), triterpenoids (mainly Ganoderma acid), etc. The medicinal value of Ganoderma lucidum polysaccharide mainly includes anti-cancer, hypoglycemic, immune regulation, promotion of protein and nucleic acid synthesis, anti-inflammatory and radiation protection; the medicina...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/04C12N1/14C08B37/00A61K36/074A61P35/00
Inventor 杨义芳杨国红金隽迪胡海峰张琴
Owner SHANGHAI INST OF PHARMA IND CO LTD
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