Chinese Ganoderma fermentation method and Chinese Ganoderma mycelium prepared thereby
A fermentation method and mycelium technology, applied in the direction of fungi, etc., can solve problems such as few studies
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Embodiment 1
[0025] The preparation of embodiment 1 Zizhi mycelia
[0026] The Zizhi strain CGMCC5.69 was transferred to a PDA agar slant (containing 35% potato, 2.5% glucose, 0.3% malt extract and 1.7% agar, and the balance was water), and cultured at 25°C for 6 days to obtain a seed slant for fermentation research; Transfer to 3 750ml shake flasks with 100ml seed culture medium; The seed culture medium consists of: bean cake powder 2%, glucose 2.5%, anhydrous magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, and the balance is water, The pH is natural; cultivated at 25°C and 220rpm for 4 days, according to the inoculum size of 10v / v%, 100ml seed solution was transferred to 10 750ml shake flasks with 100ml fermentation medium (fermentation medium composition: 4% sucrose, peptone 0.4%, yeast extract 0.2%, anhydrous magnesium sulfate 0.15%, potassium dihydrogen phosphate 0.3%, the balance is water, pH6.0), 28 ℃, 190rpm shaker culture for 5 days. After the fermentation is over, ...
Embodiment 2
[0027] The preparation of embodiment 2 Zizhi mycelium
[0028] Inoculate Zizhi strain ACCC50045 on PDA slant medium (containing 30% potato, 3% glucose, 0.5% malt extract and 1.4% agar), culture at 25°C for 7 days, transfer to 4 seeds containing 100ml of medium to obtain 750ml Triangular shake flask (seed medium: 1% bean cake powder, 5% glucose, 0.30% anhydrous magnesium sulfate, 0.1% potassium dihydrogen phosphate, natural pH), 28°C, 200rpm for 6 days, according to 5v / v% inoculum Transfer to ten 750ml Erlenmeyer flasks with 100ml fermentation medium, culture at 25°C and 220rpm for 7 days, fermentation medium: 2.5% sucrose, 0.6% peptone, 0.1% yeast extract, 0.10% anhydrous magnesium sulfate, phosphoric acid Potassium dihydrogen 0.5%, pH6.5. Mycelium separation is the same as in Example 1; the weight is 0.23g / ml fermentation broth.
Embodiment 3
[0029] The preparation of embodiment 3 Zizhi mycelia
[0030] Inoculate Zizhi strain ACCC50468 on PDA slant medium (containing 40% potato, 2% glucose, 0.2% malt extract and 2.0% agar), culture at 25°C for 7 days, transfer to 4 seeds containing 100ml of medium to obtain 750ml Triangular shake flask (seed medium: 3% bean cake powder, 1.5% glucose, 0.30% anhydrous magnesium sulfate, 0.4% potassium dihydrogen phosphate, natural pH), 26°C, 200rpm for 4 days, according to 15v / v% inoculum size Transfer to ten 750ml Erlenmeyer flasks with 100ml fermentation medium, culture at 26°C and 220rpm for 4 days, fermentation medium: 6% sucrose, 0.2% peptone, 0.3% yeast extract, 0.3% anhydrous magnesium sulfate, phosphoric acid Potassium dihydrogen 0.2%, pH5.5. Mycelium separation is the same as in Example 1; the weight is 0.18g / ml fermentation broth.
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