Method for obtaining haploid plant of calla by free microspore culture
A technology of microspore culture and single calla lily, applied in the fields of botanical equipment and methods, horticultural methods, plant cells, etc., can solve problems such as no public reports, and achieve the effect of accelerating the process of purification and breeding
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[0016] 1. Suitable growth conditions for the donor calla lily material.
[0017] The suitable growth season for the donor calla lily material is autumn and winter, and the suitable growth temperature is the highest temperature of 25°C and the lowest temperature of 10°C. For example, the suitable season for open field cultivation of calla lilies in Kunming is autumn (September-November).
[0018] 2. Appropriate development period for in vitro culture of microspores.
[0019] There is a correlation between the developmental stages of flower buds, anthers and microspores, and the appropriate developmental stage is an important factor affecting the culture effect of free microspores and whether monoculture plants of Calla lily can be successfully obtained. When the microspores are in the uninucleate marginal stage, it is the best period for the free microspores of calla lily to cultivate. Cultivation can obtain calla lily monoculture plants (Table 1).
[0020] Table 1 The suita...
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