Method for obtaining haploid plant of calla by free microspore culture

A technology of microspore culture and single calla lily, applied in the fields of botanical equipment and methods, horticultural methods, plant cells, etc., can solve problems such as no public reports, and achieve the effect of accelerating the process of purification and breeding

Inactive Publication Date: 2009-03-18
YUNNAN UNIV
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Problems solved by technology

[0005] Through document retrieval, do not see the public report identical with the technical content of the present invention

Method used

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Embodiment Construction

[0016] 1. Suitable growth conditions for the donor calla lily material.

[0017] The suitable growth season for the donor calla lily material is autumn and winter, and the suitable growth temperature is the highest temperature of 25°C and the lowest temperature of 10°C. For example, the suitable season for open field cultivation of calla lilies in Kunming is autumn (September-November).

[0018] 2. Appropriate development period for in vitro culture of microspores.

[0019] There is a correlation between the developmental stages of flower buds, anthers and microspores, and the appropriate developmental stage is an important factor affecting the culture effect of free microspores and whether monoculture plants of Calla lily can be successfully obtained. When the microspores are in the uninucleate marginal stage, it is the best period for the free microspores of calla lily to cultivate. Cultivation can obtain calla lily monoculture plants (Table 1).

[0020] Table 1 The suita...

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Abstract

The invention relates to a method for culturing and obtaining the monoploid plant of Zantedeschia aethiopica with free microspores, belonging to the technical field of flower breeding. The steps of the method comprise that the Zantedeschia aethiopica stamen with the microspore being at the late nonnuclear stage is fetched for sterilization; the microspore is free mechanically and the cell density is adjusted to 1.0*10<5> microspores / ml with NLN-13% fructose and then the free microspores are pre-cultured in the dark under the temperature of 32 DEG C for two days; and then the microspores are pre-cultured in the dark under the temperature of 25 DEG C for five days and the culture medium is changed for the culture medium of NLN-13% sucrose after culture; continuously the microspores are cultured in the dark under the temperature of 25 DEG C and the microspores are developed into callus; the callus forms the monoploid plant by bud differentiation. The monoploid plant of Zantedeschia aethiopica obtained by the method needs only one generation to obtain the pure strain with stable heredity by doubling the chromosome, which is an approach for accelerating the purification of breeding materials and process effectively.

Description

Technical field: [0001] The invention relates to a method for obtaining monoculture plants of calla lily (Zantedeschia aethiopica) through free microspore culture, belonging to the technical field of flower breeding. Background technique: [0002] Calla lily (Zantedeschia aethiopica) is a perennial bulbous flower of the genus Zantedeschia in the family Araceae. It is an excellent fresh-cut flower and potted flower; the traditional variety of calla lily has white flowers, but there are other gorgeous flowers in this species currently on the market. Varieties of colorful flowers, calla lilies with high purity and bright white or bright colors and elegant shapes occupy an important position in the flower market. [0003] In the prior art, a pedigree method is used to obtain a genetically stable pure line of calla lily, and the method requires 6-7 generations (15-20 years) to obtain a genetically stable pure line of calla lily. This method not only takes a long time, but also c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 张乐民王世敏杨丽丽郑思乡
Owner YUNNAN UNIV
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