Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes

A deer faceplate and gel filtration chromatography column technology, applied in the field of biomedicine, to achieve excellent hypoglycemic effect

Inactive Publication Date: 2009-04-08
CHINA PHARM UNIV
View PDF0 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports on the structure of the active protein of the flower disc and the research work on the anti-diabetic active substances of velvet and flower disc. Therefore, this study is in line with the development direction of the modernization of traditional Chinese medicine in my country, and has important social significance and potential economic benefits in the field of basic research and applied research. benefit

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes
  • Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes
  • Polypeptide from deer floral disc, preparing method and uses for preventing and treating diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of centrifuged supernatant: Take 5kg of deer discs, crush them, add 15,000ml of pre-cooled pH3.5 acetic acid solution, stir at 4°C for 30 minutes, and then -1 Refrigerate and centrifuge for 20 minutes under conditions, take the supernatant, and repeat the extraction of the precipitate once.

[0027] The above supernatants were combined, then filtered with Xinhua filter paper, the filtrate was collected, and the protein content was measured by the biuret method to be 11.194 mg / ml.

[0028] Superdex with FPLC TM G75 (purchased from Amfarmersia Biotechnology (China) Co., Ltd.) gel filtration chromatography column for purification, after the above supernatant was freeze-dried, 1ml of the sample (concentration: 50mg / ml) was loaded, eluted with distilled water, and collected Peak 2, its protein content measured by biuret method is 1.487mg / ml.

[0029] Take the RESOURSE of the FPLC on peak 2 above TM RPC3ml column, load 1ml (concentration is 1mg / ml), mobile pha...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention relates to the bio-medical field, particularly to a polypeptide separated from a deer antler base by using bioseparation engineering technology for preventing and treating diabetes. The relative molecular weight of the polypeptide is 7,127.6 daltons, the UV characteristic absorption peak is 205nm, lavender is shown on biurets reaction, and royal purple is shown on ninhydrin reaction. The polypeptide contains 16 kinds of amino acid. The polypeptide has good thermal stability and is stable in pH between 2 and 9. The polypeptide of the deer antler base is proved to have excellent effect of blood glucose reduction by the experiment of pharmacodynamics in vivo.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a polypeptide for preventing and treating diabetes from deer flower discs by using bioseparation engineering technology. Background technique [0002] Deer discs, also known as horn discs and pearl discs, are the antler feet that are left on the upper part of the horn base of sika deer or red deer when they saw the antlers. Folks have a long history of using antler discs to treat hyperplasia of mammary glands. Therefore, the chemical composition and pharmacological effects of the flower disc are similar to those of deer antler. The reported pharmacological effects of flower disc powder include: inhibiting mammary gland hyperplasia, anti-fatigue, improving the stress response caused by harmful factors, enhancing the body's hematopoietic function, anti-inflammatory, antibacterial and analgesic, promoting the phagocytosis of mouse macrophages and T Lymphocyte proliferation ability, inhi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/47C07K1/20C07K1/16A61K38/17A61P3/10
Inventor 吴梧桐黄凤杰钱璟吉静娴
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap