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ELISA reagent kit for screening suspected patient with immunity correlated disease

A technology of immune-related diseases and enzyme-linked immunosorbent reagents, which is applied in the field of enzyme-linked immunosorbent immunoassay kits, and can solve problems such as induction

Inactive Publication Date: 2013-03-20
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] There are few studies on the role of antibodies after TCV immunization, but current studies have shown that after immunization with activated T cells, the host can produce antibodies against cell surface activation molecules, and BDA / IJ mice were inoculated with specific T cell lines targeting human type II collagen , can induce resistance to arthritis in mice, and found that there is also an anti-idiotypic antibody in mouse serum, which can react with autoantibodies against human type II collagen

Method used

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  • ELISA reagent kit for screening suspected patient with immunity correlated disease
  • ELISA reagent kit for screening suspected patient with immunity correlated disease
  • ELISA reagent kit for screening suspected patient with immunity correlated disease

Examples

Experimental program
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Embodiment 1

[0063] Example 1. Target proteins recognized by anti-activated T cell antibodies are calreticulin (CRT), ERp57 and vimentin

[0064] In the process of studying the mechanisms of TCV-induced immune regulation, people have gained a better understanding of TCV-induced regulatory T cell responses, but little is known about TCV-induced specific humoral responses (ie, antibodies). In the present invention, the ovalbumin (OVA)-specific T cell line derived from BALB / c mice was used as a vaccine to immunize mice of the same strain, and it was found that TCV could induce a humoral immune response against activated T cells. This result reveals the existence of anti-T cell regulatory antibodies, lays the foundation for future research on regulatory anti-T cell antibodies (regulatory anti-T-cell Abs), and also proposes a new approach for the treatment of immune-related diseases through this approach. New strategies and methods.

[0065] 1. Establishment of OVA-specific T cell lines

[00...

Embodiment 2

[0102] Example 2, ELISA kit for screening suspected patients with immune-related diseases

[0103] The present invention develops CRT, ERp57 and Vimentin specific antibody detection ELISA kit, and uses it to measure serum antibodies of chronic hepatitis B patients, rheumatoid arthritis patients and SLE patients.

[0104] 1. The composition of the kit

[0105] The composition of the kit is mainly composed of calreticulin-1, ERp57-1 or vimentin-coated ELISA plates, and also contains anti-HRP-labeled human or mouse IgG, and various reagent solutions for conventional ELISA detection: washing solution, substrate solution and stop solution.

[0106] Wherein, calreticulin-1, ERp57-1 or vimentin recombinant protein is coated with the microtiter plate according to the following method: use coating solution (0.05M pH9.6 carbonate buffer) to calreticulin-1, ERp57-1 or vimentin recombinant protein Dilute to 0.1-1 μg / ml respectively, add 100 μl to each well, incubate at 37°C for 2 hours,...

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Abstract

The invention discloses an enzyme-linked immunoassay kit used for screening immune-related disease suspected patients, comprising an antigen and an enzyme-marked second antibody; the antigen is at least one of the following proteins or polypeptide: 1) the polypeptide obtained from calreticulin protein loss and at least containing amino acid residues from the 21st bit to the 253rd bit of the amido end of GenBank Accession Number AAH03453; 2) the polypeptide obtained from ERp57 protein loss and at least containing the amino acid residues from the 27th bit to 250th bit of the amido end of GenBank Accession Number P27773; 3) vimentin protein; 4) calreticulin protein; and 5) ERp57 protein. The kit can be combined with other critical information and verifies mutually and improves the diagnosis efficiency.

Description

technical field [0001] The invention relates to an ELISA kit for screening suspected patients with immune-related diseases. Background technique [0002] The immune system controls the immune response within an appropriate intensity through positive and negative feedback regulation through multiple channels. These include the regulation of antigens, the nervous system and the endocrine system, but the one that has received the most attention and is of critical importance is the regulation of regulatory T cells. The effective integration of this multi-channel regulation ensures the homeostasis of the immune system. [0003] T cells are central to the adaptive immune response and a major target of immune regulation. Many immune diseases arise from abnormalities in T cell activation or regulation. These diseases include autoimmune diseases, allergic diseases and the like. Autoimmune diseases are a class of frequently occurring diseases that seriously endanger human health. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/53
Inventor 高晓明仲昭岩许文洪超邱翔吕平
Owner PEKING UNIV