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Preparation of Cordyceps sinensis polysaccharide injection and use in cancer treatment

A technology of Cordyceps polysaccharide and Cordyceps militaris mycelium is applied in the directions of medical preparations, pharmaceutical formulations, and plant raw materials containing active ingredients, and achieves the effects of strong activity, simple and easy production process, and wide source of raw materials.

Inactive Publication Date: 2011-10-05
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention separates and obtains a kind of Cordyceps polysaccharide with anti-tumor activity through the method of alkali extraction, and the extracted polysaccharide part has strong activity and high purity (above 90%), and its active Cordyceps polysaccharide component has not been reported before the present invention

Method used

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  • Preparation of Cordyceps sinensis polysaccharide injection and use in cancer treatment
  • Preparation of Cordyceps sinensis polysaccharide injection and use in cancer treatment
  • Preparation of Cordyceps sinensis polysaccharide injection and use in cancer treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: Preparation of Cordyceps polysaccharide

[0056] (1) Raw material processing: put the Cordyceps militaris mycelium into the pulverizer and pulverize to obtain the Cordyceps militaris mycelium coarse powder;

[0057] (2) Degreasing: take the coarse powder of Cordyceps militaris mycelium, reflux extraction in 15L petroleum ether for 4 hours, repeat twice;

[0058] (3) Alcohol extraction: reflux extraction with 20% ethanol for 3 times, each time is 3 hours;

[0059] (4) Filtration: filter with 4 layers of gauze to reclaim the residue;

[0060] (5) Alkaline extraction: Add 0.2mol / L Ca(OH) to the residue 2 Extract 3 times, 5 hours each time;

[0061] (6) Concentration: filter the combined filtrates, adjust the pH to 7.0 with acid, and concentrate under reduced pressure at 60°C to 1 / 5 of the original volume;

[0062] (7) Alcohol precipitation: add 4 times the volume of 95% ethanol to the cooled concentrated solution, centrifuge overnight at 4°C, and wash the...

Embodiment 2

[0065] (1) Degreasing: take the coarse powder of Cordyceps militaris mycelium, reflux extraction in 15L petroleum ether for 4 hours, repeat twice;

[0066] (2) Alcohol extraction: use 20% ethanol to reflux extract 3 times, each time is 3 hours;

[0067] (3) Filtration: filter with 4 layers of gauze to reclaim the residue;

[0068] (4) Alkaline extraction: adding 0.2mol / L NaOH to the residue to extract 3 times, 5 hours each time;

[0069] (5) Concentration: filter the combined filtrate, adjust the pH to 7.0 with acid, and concentrate under reduced pressure at 60°C until the liquid is viscous and hangs on the wall;

[0070] (6) Alcohol precipitation: add 4 times the volume of 95% ethanol to the cooled concentrated solution, centrifuge overnight at 4°C, and wash the precipitate with an appropriate amount of absolute ethanol;

[0071] (7) Drying: freeze-dry the precipitate obtained in step (7), and pulverize it into a fine powder to obtain Cordyceps polysaccharide.

Embodiment 3

[0073] (1) Degreasing: get 5 kg of Cordyceps militaris mycelium coarse powder, reflux extraction in 15L petroleum ether for 4 hours, repeat 2 times;

[0074] (2) Alcohol extraction: use 20% ethanol to reflux extract 3 times, each time is 3 hours;

[0075] (3) Filtration: filter with 4 layers of gauze to reclaim the residue;

[0076] (4) Alkaline extraction: add the residue to 0.5mol / LNaHCO 3 Extract 3 times, 5 hours each time;

[0077] (5) Concentration: filter the combined filtrate, adjust the pH to 7.0 with acid, and concentrate under reduced pressure at 60°C until the liquid is viscous and hangs on the wall;

[0078] (6) Alcohol precipitation: add ethanol to the cooled concentrated solution to make the final concentration of ethanol reach 80%, centrifuge overnight at 4°C, and wash the precipitate with an appropriate amount of absolute ethanol;

[0079] (7) Drying: freeze-dry the precipitate obtained in step (7), and pulverize it into a fine powder to obtain Cordyceps pol...

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Abstract

The invention discloses a preparation method of anti-tumor cordycepin polysaccharide. The preparation method comprises the following steps: Chinese caterpillar fungus mycelium is used as a raw material, is refluxed and extracted by one to three times for 4 hours every time by adding petrolic ether after being broken, then filtering is carried out, solvent is evaporated, and 20 percent of ethanol is used for refluxing extraction for twice to three times with 3 hours every time. After filtering, residue is added with alkali liquid for extraction for twice to four times with 5 hours every time; the filtrates are mixed, and the PH value is adjusted to 7.0 by acid, the filtrates are cooled after being decompressed and condensed, and 95 percent of ethanol of 4 times volume is added, and then the mixture is stood overnight under 4 DEG C and centrifuged. The cordycepin polysaccharide can be obtained by washing sediment with absolute ethanol, freeze drying and smashing to fine powders; the preparation method is easy application, convenient in operation and wide in material source, and the content of the obtained cordycepin polysaccharide is more than 85 percent, wherein, the content of neutral polysaccharide is about 61.8 percent, the content of acidic polysaccharide is about 25.7 percent, the content of polypeptide is less than 5 percent, simultaneously, the invention also provides a method for preparing an injection agent by active cordycepin polysaccharide components and the application thereof in preparing medicaments for treating or preventing tumors.

Description

technical field [0001] The invention discloses a cordyceps polysaccharide, and provides a preparation method thereof, and application of an injection containing the compound as an active ingredient in tumor treatment or prevention, and belongs to the technical field of extracting and applying effective ingredients of traditional Chinese medicines, and also belongs to the field of biochemical pharmacy. Background technique [0002] Cordyceps militaris (Cordyceps militaris) referred to in the present invention is a kind of fungus that grows on the pupal body, and is identified as Ascomyeotina (Ascomyeotina), Ergotaceae (Clariceptaceae), Cordyceps (Cordyceps) plant. According to reports, Cordyceps militaris fermented liquid has good therapeutic effects on asthma and bronchial diseases, immune regulation, and enhancement of renal function in patients with renal failure. Scholars at home and abroad have systematically studied its chemical composition and physiological activity, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61K36/068A61P35/00
Inventor 滕利荣孟庆繁逯家辉张娜王贞佐王德利刘艳王悦怡
Owner JILIN UNIV
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