Nucleic acid fluorescent quantitative RT-PCR detection kit and detection method for enterovirus Cox A16

Abstract

The invention provides a fluorescence quantitative RT-PCR detecting kit for the Coxackie A16 type(Cox A16) nucleic acid of an enterovirus and a detecting method thereof; and the sequences of an upstream primer and a downstream primer and a specific probe of the fluorescence quantitative RT-PCR detecting kit are as follows: the upstream primer CA16YGF is 5'- GGGAATTTCTTTAGCCGTGC-3', the downstream primer CA16YGR is 5'-CCC ATC AAR TCAATG TCCC-3' and the specific probe COXA16PB is 5'-ACAATG CCCACCACGGGTACACA -3'. The method has high specificity on detecting the enterovirus Cox A16 and does not have cross reactions with other enteroviruses; moreover, the detection sensitivity of the method achieves 0.1TCID50; the method can directly detect the nucleic acid of the enterovirus Cox A16 from the samples of ncurolymph, herpes liquid, dejecta and the like of suspected patients of the hand-foot-and-mouth disease; only about 3h is needed from extracting the nucleic acid of the enterovirus to finishing the detection; and the detecting kit and the detecting method are suitable for the lab early diagnosis of sudden epidemic caused by the infection of the enteroviruses Cox A16 like the hand-foot-and-mouth disease.

Description

(1) Technical field [0001] The invention relates to an enterovirus Coxsackie A16 type (Cox A16) nucleic acid fluorescence quantitative RT-PCR detection kit and a detection method, which can be applied to laboratory emergency detection of hand, foot and mouth disease and other outbreaks. (2) Background technology [0002] Hand foot mouth disease (HFMD) is a common infectious disease in infants and young children. Clinically, it is mainly characterized by fever, rashes and ulcers on the hands, feet, mouth and other parts. Individual patients can cause myocarditis, pulmonary edema, aseptic Fatal complications such as meningoencephalitis, hand, foot and mouth disease is a global infectious disease, and there are reports of the prevalence of this disease in most parts of the world. [0003] Hand, foot and mouth disease is generally an acute infectious disease caused by enterovirus. In recent years, the outbreak of hand, foot and mouth disease in China has attracted global attenti...

AI Technical Summary

Problems solved by technology

[0004] Since HFMD is caused by a variety of human enterovirus infections, among which EV 71 and Cox A16 are the most common types, in order to quickly find out the cause of the disease and take timely control measures, rapid laboratory diagnosis is urgently needed. The current detection method is virus isolation and neutralization method, which is cumbersome and time-consuming, and is not suitable for early diagnosis

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