Primer, detecting probe and actual time fluorescent PCR kit for detecting Thielaviopsls basicola

Abstract

The invention relates to a primer, a probe and a real-time fluorescent PCR reagent kit for detecting Thielaviopsls basicola. The primer comprises a forward primer Tb1 and a reverse primer Tb2, and base sequences of the forward primer Tb1 and the reverse primer Tb2 are Tb1: 5'-ACC ATA TGT GAA CGT ACC TTT TCT-3' and Tb2: 5'-AGT TTA TAA ATG CTA CCG GCA GAA-3' respectively. A base sequence of the probe is any one nucleotide sequence in an area that shifting two basic groups toward an upstream or shifting three basic groups toward a downstream at both ends of 5'-CCC GAG AGG CAC CTG CCA AAG CA-3'; on the nucleotide sequence, a fluorescent reporter group is labeled at a 5' end, and a fluorescent quencher group is labeled at a 3' end to form the fluorescence-labeled probe; and the primer and the probe are used to be prepared into the corresponding reagent kit. The invention can quickly and accurately detect the Thielaviopsls basicola in field sick soil, thereby providing an accurate evidence for promptly adopting measures to cut off infection sources of plant diseases; and the invention can also quickly and accurately detect the plant diseases on plants.

Description

technical field [0001] The present invention relates to a bioengineering technology suitable for agricultural production, plant protection and other departments, in particular to a primer, a probe and Real-time fluorescent PCR kit. Background technique [0002] Tobacco root black rot is one of the important diseases of tobacco. It spreads all over the world's main tobacco producing areas. It has caused serious losses in tobacco production in the United States, Canada, Japan and other countries. It also occurs in my country's main tobacco producing areas. Especially in recent years, the disease has spread in some smoking areas, and it has occurred more seriously in Yunnan, Guizhou, Hubei and other provinces of my country, and the incidence rate of severe plots can reach more than 30%. Shandong, Henan, Anhui, Jilin, Fujian and other provinces also have occurred, and there is a trend of aggravating the harm in recent years. [0003] At present, the detection methods for plant...

AI Technical Summary

Problems solved by technology

At present, in the real-time fluorescent PCR detection of tobacco root black rot, 5'-ACC ATA TGT GAA CGT ACC TTTTCT-3' and 5'-AGT TTA TAA ATG CTA CCG GCA GAA-3' are used as primers, and 5' -CCC GAG AGG CACCTG CCA AAG CA-3' sequence is used as probe to prepare corresponding real-time fluorescent PCR kit, there is no related report

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