Protein feedstuff and preparation method thereof
A protein feed and auxiliary material technology, applied in animal feed, animal feed, additional food elements, etc., can solve the problems of protein feed that have not been found, so as to alleviate the shortage of feed protein, improve feed intake speed, crude protein content and protease activity increased effect
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Embodiment 1
[0034] Embodiment 1, preparation and performance measurement of protein feed
[0035] 1. Preparation of protein feed
[0036] 1. Preparation of fermentation medium
[0037] Take 10 g of linseed meal with a particle size of 20-40 mesh that is pulverized with a grease grinder, and add it to a 250 mL conical flask. A buffer solution with a pH of 2.0 is added, and the mass ratio of the linseed meal to the buffer solution is 1:7. Obtain the fermentation medium.
[0038] 2. Culture of strains
[0039] (1) Preparation of slant strains
[0040] Potato glucose slant medium or 5Be°wort slant medium was used to inoculate Aspergillus oryzae (Asperillus oryzae 3.951, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences) in strict accordance with aseptic procedures, and cultured in an incubator at 28°C for 48 hours to make slant bacteria. species, stored in the refrigerator for later use.
[0041] (2) Bacteria culture
[0042] Using potato dextrose liquid...
Embodiment 2
[0052] Embodiment 2, preparation and performance measurement of protein feed
[0053] 1. Preparation of protein feed
[0054] 1. Preparation of fermentation medium
[0055] Take 10 g of linseed meal with a particle size of 100-120 mesh that is pulverized with a grease grinder, and add it to a 250 mL conical flask. A buffer solution of pH 10.0 was added, and the mass ratio of flax meal to buffer solution was 1:7. Obtain the fermentation medium.
[0056] 2. Culture of strains
[0057] (1) Preparation of slant strains
[0058] Potato glucose slant medium or 5Be°wort slant medium was used to inoculate Aspergillus oryzae (Asperillus oryzae 3.951, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences) in strict accordance with aseptic procedures, and cultured in an incubator at 28°C for 48 hours to make slant bacteria. species, stored in the refrigerator for later use.
[0059] (2) Bacteria culture
[0060] Using potato dextrose liquid culture medi...
Embodiment 3
[0070] Embodiment 3, preparation and performance measurement of protein feed
[0071] 1. Preparation of protein feed
[0072] 1. Preparation of fermentation medium
[0073] Take 10 g of linseed meal with a particle size of 20-40 mesh that is pulverized with a grease grinder, and add it to a 250 mL conical flask. According to the mass of linseed meal, add 10% bran, 1.0% ammonium sulfate and 0.5% dipotassium hydrogen phosphate, and mix well. A buffer solution of pH 6.0 is added, and the mass ratio of flax meal to buffer solution is 1:6. Obtain the fermentation medium.
[0074] 2. Culture of strains
[0075] (1) Preparation of slant strains
[0076] Potato glucose slant medium or 5Be°wort slant medium was used to inoculate Aspergillus oryzae (Asperillus oryzae 3.951, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences) in strict accordance with aseptic procedures, and cultured in an incubator at 28°C for 48 hours to make slant bacteria. species...
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