Shuang Huanglian liquid preparation and method for measuring content thereof
A technology of liquid preparations and measurement methods, which is applied in the direction of anti-inflammatory agents, antiviral agents, and measuring devices, which can solve problems such as prolonging detection time, increasing detection costs, and increasing detection workload, so as to improve detection efficiency and reduce detection costs , reduce the effect of allergic reactions
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[0016] Example 1
[0017] 1) Preparation of honeysuckle extract
[0018] Weigh the honeysuckle, add water to warm it for 2-3 times, filter, concentrate, alcohol precipitation, filter, the filtrate recovers ethanol to no ethanol taste, add 2-6 times the amount of purified water of the paste weight, gently stir, stand still, take it The clear liquid is filtered, the filtrate is concentrated and then alcohol-precipitated again, and the supernatant is filtered and concentrated to obtain the honeysuckle extract.
[0019] Detected by high performance liquid chromatography, it was determined that the extract contained chlorogenic acid 15.22mg / g and luteolin 0.38mg / g.
[0020] 2) Preparation of Forsythia Suspense Extract
[0021] Weigh forsythia, add water to decocting for 3 times, combine the medicinal solution, and the subsequent steps are the same as those after honeysuckle warm soaking to obtain forsythia extract.
[0022] Detected by high performance liquid chromatography, it was det...
Example Embodiment
[0026] Example 2
[0027] The three extracts obtained in Example 1 were used to prepare the following formula:
[0028] Preparation of injection (each injection is 10ml)
[0029] 1) Formulation 1: Chlorogenic acid 0.4mg / ml, baicalin 6.0mg / ml, forsythin 0.1mg / ml, wogonin 0.09mg / ml, luteolin 0.01mg / ml.
[0030] 2) Formulation 2: Chlorogenic acid 1.0mg / ml, baicalin 10.0mg / ml, forsythin 0.8mg / ml, wogonin 0.15mg / ml, luteolin 0.024mg / ml.
[0031] 3) Formulation 3: Chlorogenic acid 1.5mg / ml, baicalin 9.0mg / ml, forsythin 0.3mg / ml, wogonin 0.13mg / ml, luteolin 0.04mg / ml.
[0032] 4) Formulation 4: Chlorogenic acid 2.0mg / ml, baicalin 8.0mg / ml, forsythin 0.5mg / ml, wogonin 0.12mg / ml, luteolin 0.05mg / ml.
[0033] The preparation of oral liquid (the volume of each oral liquid is 10ml)
[0034] 5) Formulation 5: chlorogenic acid 0.6mg / ml, baicalin 8.0mg / ml, forsythin 0.3mg / ml, wogonin 0.12mg / ml, luteolin 0.015mg / ml.
[0035] 6) Formulation 6: chlorogenic acid 1.0 mg / ml, baicalin 12.0 mg / ml, forsy...
Example Embodiment
[0038] Example 3
[0039] Protective effect of Shuanghuanglian liquid preparation on influenza virus infection in mice
[0040] Medicine: Shuanghuanglian injection of the present invention (formulation 1, new Shuanghuanglian 1): chlorogenic acid 0.4mg / ml, baicalin 6.0mg / ml, forsythin 0.1mg / ml, wogonin 0.09mg / ml, Luteolin 0.01mg / ml.
[0041] Shuanghuanglian oral liquid of the present invention (formulation 5, new Shuanghuanglian 2): chlorogenic acid 0.6mg / ml, baicalin 8.0mg / ml, forsythin 0.3mg / ml, wogonin 0.12mg / ml, luteolin Glycoside 0.015mg / ml.
[0042] Commercially available Shuanghuanglian injection: chlorogenic acid 1.3mg / ml, baicalin 7.2mg / ml, forsythin 0.04mg / ml, wogonin 0.02mg / ml, luteolin 0.003mg / ml. .
[0043] Commercially available Shuanghuanglian oral liquid: chlorogenic acid 1.1mg / ml, baicalin 8.4mg / ml, forsythin 0.4mg / ml, wogonin 0.04mg / ml, luteolin 0.002mg / ml.
[0044] Animals: ICR closed group mice, body weight (20±2)g, half male and half female
[0045] Virus strai...
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