Synthetic apparatus for constructing tissue engineering seed cell and scaffold complex

Abstract

A synthesizer for constructing a compound of tissue engineering seeded cells and a scaffold is assembled by a raw material tank, an alkaline solution tank, a seeded cell suspension A tank, an air filter, a peristaltic pump, a reaction kettle, a temperature controller, a mould flow plate, a seeded cell suspension B tank, a waste fluid tank, a control valve and a flow meter. At least 5 feed inlets are opened at the top part of the reaction kettle; a discharge port is opened at the bottom part of the reaction kettle; a blender, a temperature electrode and a ph electrode are installed in the reaction kettle; a constant temperature jacket is arranged on the periphery of the kettle body; an upper jacket port and a lower jacket port are opened on the constant temperature jacket; and a layered plate, in which wire net moulds are distributed uniformly, is placed in the mould flow plate. The invention has the advantages of simple structure, convenient operation, high synthetic efficiency, strong expansibility and commonality. The invention can provide a matched cell scaffold production device for the large-scale production of tissue engineering products and meet the equipment requirements of the tissue engineering product production.

Description

technical field [0001] The invention belongs to production equipment of medical biological materials, in particular to a synthesis device for constructing tissue engineering seed cells and scaffold complexes. Background technique [0002] Tissue engineering is an emerging interdisciplinary field developed in the past 30 years. Its ultimate goal is to produce products for restoring, maintaining, or enhancing the structure and function of human damaged organs or tissues, such as tissue engineered skin, cartilage, tendons and blood vessels etc. Tissue-engineered skin is the fastest-growing category of products, and several products are currently in clinical use. To produce tissue engineering products such as tissue engineering skin, cartilage, tendon and blood vessels, it is first necessary to synthesize a collagen gel-like cell-scaffold complex with collagen and mucopolysaccharides as matrix materials, so that the collagen-gel-like cell-scaffold Into the mold and then in the...

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Problems solved by technology

In the prior art, for the preparation method of the cell scaffold material for the production of tissue engineering products, there are many patent literature reports, for example, on October 31, 2001, there was a piece titled "Composite Collagen-Based Tissue Engineering Scaffold and Its Preparation Method" CN1319436A of CN1319436A, on February 5th, 2003, a piece of CN1394655A titled "Preparation Method of Collagen / Chitosan Porous Scaffold for Tissue Engineering" was disclosed, and a piece of title "Tissue Engineering Scaffold Material and Its Method" was disclosed on June 18th, 2003. "Preparation Method" CN1424115A; however, for the device or equipment for the production of cell scaffold materials for tissue engineering products, there is no patent literature report

It is difficult to achieve large-scale production of tissue engineering products without production equipment or equipment for tissue engineering product cell scaffold materials

Ding Chunmei reported a simple perfusion cell seeding device (Ding CM, Zhou Y, He YN, Tan WS. Perfusion seeding of collagen-chitosan sponges for dermal tissue engineering. Process Biochemistry2008, 43: 287-296), which consists of a The seed cell bottle, an inoculation chamber, a peristaltic pump and pipelines, the inoculation chamber consists of two Erlenmeyer flasks placed upright at the bottom, with a filter, collagen-chitin sponge bracket and gasket in the middle from bottom to top, The gasket plays the role of restricting the flow of the seed cell suspension through the sponge support and the filter screen. When the seed cells flow through the sponge support at a certain flow rate (such as 0.25ml / min) through the peristaltic pump, they will adhere to the support, thereby realizing the separation between the cells and the support. The composite, non-adherent cells flow back to the seed cell bottle, and can be inoculated on the scaffold in the next cycle. However, this device can only inoculate cells in small batches, and only one scaffold can be inoculated at a time, and the scaffold area is small. Multiple cycles increase cell death

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