Reagent kit used for predicting chronic obstructive pulmonary disease
A technology for chronic obstructive pulmonary disease and a kit, applied in the biological field, can solve problems such as the status and course of chronic obstructive pulmonary disease that cannot be reflected overall, and the research on lung function is lagging behind, and achieve the effect of simple, easy and reliable protein expression level.
Inactive Publication Date: 2009-07-15
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology
[0004] The technical problem to be solved by the present invention is to change the situation that lung function research lags behind and cannot reflect the state and course of chronic obstructive pulmonary disease as a whole, and develop a test kit for predicting chronic obstructive pulmonary disease
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Embodiment 1
[0020] A kit for predicting chronic obstructive pulmonary disease, consisting of cytokines and their enzyme-labeled antibodies, pH9.6 carbonate buffer, pH7.4 phosphate buffer, serum protein dilution, stop solution, tetramethyl Composition of benzidine substrate solution, normal human serum and positive control serum.
[0021] The cytokines are Wnt inhibitory factor 1, tumor necrosis factor β, macrophage inflammatory protein 3α, tumor necrosis factor superfamily member 10, vascular endothelial growth factor D receptor, eosinophil chemoattractant protein 3 and chemoattractant Kin factor ligand 26 and glucocorticoid-induced TNF receptor family related proteins.
[0022] The pH9.6 carbonate buffer is obtained by adding 1.59 grams of sodium carbonate and 2.93 grams of sodium bicarbonate to 1000 ml of distilled water.
[0023] Described pH7.4 phosphate buffer solution is that 0.2 gram potassium dihydrogen phosphate, 2.9 gram disodium hydrogen phosphate dodecahydrate, 8.0 gram sodiu...
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The invention provides a kit for forecasting chronic obstructive pulmonary disease, composed of cytokine and enzyme-labeled antibody thereof, pH9.6 carbonate buffer, pH 7.4 phosphate buffer, serum protein diluent, termination liquid, tetramethyl benzidine substrate solution, normal human serum and positive control serum, characterized in that: the cytokine is Wnt inhibitor 1, tumor necrosis factor Belta, macrophage inflammatory protein 3 Alpha, tumor necrosis factor super-family member 10, vascular endothelial growth factor D receptor, eosinophilic granulocyte chemotactic protein-3 and chemotactic factor ligand 26 and glucocorticoid-induced TNF receptor family-related protein. The kit obviously precedes the clinical index for precasting the chronic obstructive pulmonary disease, suitable for enzyme linked immunosorbent assay. The kit can be also changed as protein chip technique and the operation is very simple.
Description
technical field [0001] The invention relates to a kit for predicting chronic obstructive pulmonary disease, which belongs to the field of biotechnology. Background technique [0002] The determination of protein in serum has been used in the diagnosis of chronic obstructive pulmonary disease for nearly ten years (using serum amyloid A and C reactive protein to diagnose the acute attack stage of chronic obstructive pulmonary disease). It is very convenient to use serum amyloid A and C reactive protein to diagnose acute exacerbation of chronic obstructive pulmonary disease by enzyme-linked immunoassay, but it is insensitive and non-specific, and has insurmountable shortcomings. Pulmonary function tests are important for determining airflow limitation, but pulmonary function lags behind and cannot reflect the state and course of COPD as a whole, which has become a bottleneck for improving the diagnosis, prevention and treatment of COPD. [0003] The role of systemic changes in...
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Inventor 白春学王向东王耀丽蒋进军宋振举李卡
Owner ZHONGSHAN HOSPITAL FUDAN UNIV