Application of T-2 toxin in preparing medicament for treating solid tumor

A technology for solid tumors and toxins, applied in the direction of antineoplastic drugs, drug combinations, active ingredients of heterocyclic compounds, etc., can solve the problems that have not been discovered in the research of T-2 toxins

Active Publication Date: 2009-07-22
山东肽莱生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the research on T-2 toxin mainly focuses on its toxicity research and pathogenicity research on animals, and there is no report on the research on T-2 toxin used in the treatment of solid tumors.

Method used

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  • Application of T-2 toxin in preparing medicament for treating solid tumor
  • Application of T-2 toxin in preparing medicament for treating solid tumor
  • Application of T-2 toxin in preparing medicament for treating solid tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1: Experimental research on the therapeutic effect of T-2 toxin on pancreatic cancer

[0015] Human pancreatic cancer tissue fragments with a diameter of 2-3 mm were subcutaneously transplanted into mice. After the tumor grew to 6-7 mm, T-2 toxin dissolved in ethanol saline mixture was injected into the tumor at a dose of 2.5 mg / kg body weight. injection, and the control group was injected with normal saline. The size of the tumor was measured with a vernier caliper or a standard template on the 1st, 5th, 9th, 14th, 19th, and 24th day after injection, and the measurement lasted for 24 days. After 24 days of treatment, the tumor volume of the animals in the T-2 toxin group was only 52.19% of that in the control group.

Embodiment 2

[0016] Example 2: Experimental research on the therapeutic effect of T-2 toxin on lung cancer

[0017] Mouse Lewis lung cancer cells were placed in complete culture medium containing 10% fetal bovine serum and DMEM, at 37°C, 5% CO 2 Cultivate in an incubator, and replace the culture medium every 2-3 days. The cells grow adherently in a single layer, digested with 0.5% trypsin and passaged. After the total number of cells reaches the requirement, the number of living cells determined by the trypan blue exclusion method is greater than 95%, and the cell concentration is adjusted to 5×10 6 / ml, cell suspension spare C57BL / 6 pure line mice, male, 20, 6-8 weeks old, body weight (18±1) g. Inoculate subcutaneously in the axilla. Each mouse was inoculated with 0.2ml, and the cell count was 5×10 6 cells / ml. Mice were randomly divided into 2 groups, 10 in each group. In the control group, 2 ml of normal saline was injected into the tumor, and in the experimental group, T-2 toxin d...

Embodiment 3

[0021] Example 3: Experimental research on the therapeutic effect of T-2 toxin on liver cancer

[0022] Mouse H 22 Liver cancer cell lines, ICR mice peritoneal passage. Under aseptic conditions, the H 22 For liver cancer cells, adjust the cell concentration to 5×10 with normal saline 6 / ml, 20 detergent ICR mice, 6-8 weeks old, male, 18-22g, were randomly divided into 2 groups, 10 in each group. 0.2 ml of tumor cell suspension was inoculated subcutaneously in the left armpit. Seven days after the inoculation, the control group was injected with 2 ml of normal saline, and the experimental group was injected with T-2 toxin dissolved in ethanol-normal saline mixture at a dose of 2.0 mg / kg body weight. The long diameter (L) and short diameter (W) of the tumor were measured with a vernier caliper every other day after group administration, and the tumor volume and tumor growth inhibition rate were calculated. The results are shown in Table 2. The test results showed that T-2 t...

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Abstract

The invention discloses novel application of a T-2 toxin, namely the application of the T-2 toxin in preparing a medicine treating solid tumor. The effective curative dose is 0.1 to 20mg / Kg(weight), and the preferred dose is 0.5 to 8mg / Kg(weight). The administration mode can be implantation administration, oral administration, intravenous injection administration, hypodermic injection administration and intramuscular injection administration, and the preferred administration is intra-tumor injection. By establishing various solid tumor models, the inventor of the application inspects the inhibiting effects of the T-2 toxin on tumors or tumor cells in the modes of intra-tumor injection of the T-2 toxin or the co-culture of the T-2 toxin and cells, and the leukaemia is treated by utilizing the toxic effects of the T-2 toxin on tissues vigorously divided. The results show that the intra-tumor injection has definite effects of treating tumors, in particular difficultly ablated solid tumors; moreover, by the intra-tumor injection administration, range and degree of the side effects of the T-2 toxin are further lowered, so that the side effects are lowered, and the effects of inhibiting and killing tumor tissues are improved.

Description

technical field [0001] The invention relates to the application of T-2 toxin in the preparation of medicines for treating solid tumors. Background technique [0002] Tumor is one of the common diseases that threaten human health, second only to cardiovascular disease. The research on tumor has made great progress, and a variety of radiotherapy, chemotherapy and other methods have been developed to treat tumors. However, various drugs and therapies There are limitations, it is difficult to achieve a radical effect, and prone to drug resistance and side effects. The mortality rate of cancer still ranks first in various diseases. According to statistics, in 2006, 3 million people died of cancer in my country, and the incidence of cancer is still on the rise, and there is a trend of getting younger. According to statistics, in less than 20 years, the incidence of cancer in my country has increased by 69%, and the mortality rate has increased by 29.4%. Therefore, the study of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/35A61P35/00
Inventor 厉保秋
Owner 山东肽莱生物制药有限公司
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