Bacillus subtilis and method for preparing gamma-polyglutamic acid by using the same

A technology of Bacillus subtilis and polyglutamic acid, applied in the field of Bacillus subtilis and the preparation of γ-polyglutamic acid by the bacteria, can solve the problems of long cycle, low production efficiency, high cost, etc., and achieve increased output and reduced viscosity , the effect of low concentration

Inactive Publication Date: 2009-07-22
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, although the production capacity of strains has been greatly improved, its high cost, long cycle and low production efficiency are obstacles to the industrial scale production of γ-polyglutamic acid

Method used

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  • Bacillus subtilis and method for preparing gamma-polyglutamic acid by using the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Bacillus subtilis (Bacillus subtilis) XN01 was cultured on a solid slope at a constant temperature of 37°C for 24 hours, and then stored at 2-4°C. The composition of the slant medium described therein is: 1% peptone, 0.5% yeast extract, 1% NaCl, 2.0% agar, and the pH value is 6.0.

[0042] Take about 1cm from the inclined plane 2 Bacillus subtilis (Bacillus subtilis) XN01 was inoculated into the seed medium, and cultured in a shaker flask at 37° C. for 20 hours at a rotation speed of 210 rpm. The composition of the seed culture medium is as follows: 1% glucose, 1% peptone, 0.5% yeast extract, 1% NaCl, pH 7.1-7.2, and the filling volume is 50ml / 250ml shake flask.

[0043] The seed culture medium was inserted into the fermentation medium according to the inoculum amount of 5%, and cultured in a shaker flask at 37° C. for 24 hours at a rotation speed of 210 rpm. The fermentation medium described therein consists of: 3% sucrose, 5% L-glutamic acid, 1.0% yeast extract, MgS...

Embodiment 2

[0047] Same as Example 1, the carbon source was changed to maltose with the same mass concentration, and the analysis results showed that the content of γ-polyglutamic acid in the fermentation broth was 30.1 g / L.

Embodiment 3

[0049] Bacillus subtilis (Bacillus subtilis) XN01 was activated in the same seed medium as Example 1 for 15 hours, then pressed into the shake flask fermentation medium according to 5% inoculum size, and cultured in the shake flask at 37° C. for 24 hours at a rotation speed of 210 rpm. The fermentation medium described therein consists of: glucose 3%, L-glutamic acid 5%, peptone 0.8%, NaCl 1%, MgSO 4 ·7H 2 O 0.05%, MnSO 4 ·H 2 O 0.01%, K 2 HPO 4 0.1%, the pH value is 7.4~7.5, the filling volume is 50ml / 250ml shake flask.

[0050] After the fermentation, the content of gamma-polyglutamic acid in the fermentation broth was detected according to the paper chromatography method of Example 1, and the result showed that the content was 48.0 g / L.

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Abstract

The invention discloses a bacillus subtilis and a Gamma-polyglutamic acid preparation method that uses the bacillus subtilis strain. The strain is bacillus subtilis XN01 and is preserved in China Center for Type Culture Collection (CCTCC) on March 18th, 2008 with CCTCC NO. of M 208044; the Gamma-polyglutamic acid can be prepared with the strain through the steps of strain cultivation and preservation, seed fluid preparation, shaking fluid fermentation and Gamma-polyglutamic acid extraction. The Gamma-polyglutamic acid preparation method can achieve high synthesis efficiency of Gamma-polyglutamic acid in a low-cost fluid culture medium, is simple and has strong operability.

Description

technical field [0001] The invention relates to a bacillus and a method for preparing gamma-polyglutamic acid with the bacillus, in particular to a bacillus subtilis and a method for preparing gamma-polyglutamic acid with the bacillus. Background technique [0002] γ-Poly glutamic acid (γ-PGA) is a polyamino acid condensed from D-type or L-type glutamic acid monomers with γ-carboxyl and α-amino groups. The biodegradable polymer material has the following structure (Shih I, Van Y. Bioresour Technol, 2001, 79: 207-225): [0003] [0004] The relative molecular weight of γ-polyglutamic acid generally ranges from 100,000 to 1 million, and there are a large number of peptide bonds and free carboxyl groups on its main chain, so it has good biodegradability, water solubility and edible properties, and can be used as a thickener Agent, humectant, antifreeze, drug carrier, degradable fiber, high-efficiency water-retaining agent, flocculant, feed additive, etc., has broad applicat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/125
Inventor 张军华刘建军康博文
Owner NORTHWEST A & F UNIV
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