Bacillus subtilis and method for preparing gamma-polyglutamic acid by using the same

A technology of Bacillus subtilis and polyglutamic acid, applied in the field of Bacillus subtilis and the preparation of γ-polyglutamic acid by the bacteria, can solve the problems of long cycle, low production efficiency, high cost, etc., and achieve increased output and reduced viscosity , the effect of low concentration
CN101486977AInactive Publication Date: 2009-07-22NORTHWEST A & F UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHWEST A & F UNIV
Publication Date
2009-07-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a bacillus subtilis and a Gamma-polyglutamic acid preparation method that uses the bacillus subtilis strain. The strain is bacillus subtilis XN01 and is preserved in China Center for Type Culture Collection (CCTCC) on March 18th, 2008 with CCTCC NO. of M 208044; the Gamma-polyglutamic acid can be prepared with the strain through the steps of strain cultivation and preservation, seed fluid preparation, shaking fluid fermentation and Gamma-polyglutamic acid extraction. The Gamma-polyglutamic acid preparation method can achieve high synthesis efficiency of Gamma-polyglutamic acid in a low-cost fluid culture medium, is simple and has strong operability.
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Description

technical field

[0001] The invention relates to a bacillus and a method for preparing gamma-polyglutamic acid with the bacillus, in particular to a bacillus subtilis and a method for preparing gamma-polyglutamic acid with the bacillus. Background technique

[0002] γ-Poly glutamic acid (γ-PGA) is a polyamino acid condensed from D-type or L-type glutamic acid monomers with γ-carboxyl and α-amino groups. The biodegradable polymer material has the following structure (Shih I, Van Y. Bioresour Technol, 2001, 79: 207-225):

[0003]

[0004] The relative molecular weight of γ-polyglutamic acid generally ranges from 100,000 to 1 million, and there are a large number of peptide bonds and free carboxyl groups on its main chain, so it has good biodegradability, water solubility and edible properties, and can be used as a thickener Agent, humectant, antifreeze, drug carrier, degradable fiber, high-efficiency water-retaining agent, flocculant, feed additive, etc., has broad applicat...

Claims

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