Hepatitis B nucleic acid vaccine with optimized codon

A technology of codon optimization and nucleic acid vaccine, applied in the field of hepatitis B vaccine, can solve the problems of low immunogenicity, ineffective expression of foreign genes, stimulation of host immune system, etc., and achieve the effect of increasing protein expression and level

Inactive Publication Date: 2009-08-12
邢益平 +1
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Problems solved by technology

The problem is that due to the differences in codon usage preferences between prokaryotes and eukaryotes, the foreign genes used to construct nucleic acid vaccines cannot be effectively

Method used

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  • Hepatitis B nucleic acid vaccine with optimized codon
  • Hepatitis B nucleic acid vaccine with optimized codon
  • Hepatitis B nucleic acid vaccine with optimized codon

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Embodiment Construction

[0040] The invention is a method for introducing codon optimization into the hepatitis B virus surface antigen nucleic acid vaccine to improve the immunogenicity of the existing vaccine. After codon optimization, the hepatitis B surface antigen gene can be used to encode three proteins: large protein, medium protein and small protein, among which the codon-optimized hepatitis B surface antigen protein nucleic acid vaccine has been constructed and tested in animal experiments It is proved that codon optimization can indeed improve the immunogenicity of protein nucleic acid vaccine in hepatitis B surface antigen.

[0041] Specific method instructions:

[0042] First of all:

[0043] After optimization, the surface antigen of hepatitis B virus is named: HBs / opt, referred to as S-opt;

[0044] The surface antigen of hepatitis B virus before optimization was named: HBs / adr, referred to as S-adr;

[0045] Codon-optimized hepatitis B surface antigen protein (MHBs) nucleic acid vac...

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Abstract

The invention relates to a hepatitis B virus nucleic acid vaccine optimized by codon. In the invention, hepatitis B surface antigen (HBs) gene order (adr subtype) is analyzed to find codon locus which tells the differences between the codon preferences of the gene order and the codon preferences of the mammal; the codon of the HBs gene order is replaced to obtain the surface antigen gene; the gene order is combined and expanded to obtain MHBs, Pst I, BamH I, double digestion MHBs gene and carrier pSW3891 plasmid optimized by the codon; 10ul connection system is configured to obtain a middle protein gene. The nucleic acid vaccine of the invention overcomes the defects that the differences between prokaryote and eukaryote in terms of codon preferences cause that the foreign gene can not be expressed effectively in mammal reservoir and can not generate relatively good immune sheltering effect; in addition, the invention remarkably improve protein expression of the foreign gene in the mammal reservoir, effectively stimulates immune system of the reservoir to generate relatively good immunological reaction of human body fluids and cellular immune response.

Description

technical field [0001] The invention relates to a hepatitis B vaccine, in particular to a codon-optimized hepatitis B virus nucleic acid vaccine. Background technique [0002] Before the present invention, along with the development of science, nucleic acid vaccine was paid more and more attention to as a kind of novel vaccine gradually. At present, most of the target genes used to construct nucleic acid vaccines come from prokaryotes such as viruses or bacteria, while the research and application objects of vaccines are mainly eukaryotes, such as mice, macaques or human beings and other advanced mammals. The problem is that due to the differences in codon usage preferences between prokaryotes and eukaryotes, the foreign genes used to construct nucleic acid vaccines cannot be effectively expressed in mammalian hosts, and therefore cannot effectively stimulate the host's immune system. This is the main reason for the low immunogenicity of current nucleic acid vaccines. Con...

Claims

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Application Information

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IPC IPC(8): A61K39/29A61P1/16A61P31/20
Inventor 邢益平王世霞卢山黄祖瑚
Owner 邢益平
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