Hepatitis B nucleic acid vaccine with optimized codon

Abstract

The invention relates to a hepatitis B virus nucleic acid vaccine optimized by codon. In the invention, hepatitis B surface antigen (HBs) gene order (adr subtype) is analyzed to find codon locus which tells the differences between the codon preferences of the gene order and the codon preferences of the mammal; the codon of the HBs gene order is replaced to obtain the surface antigen gene; the gene order is combined and expanded to obtain MHBs, Pst I, BamH I, double digestion MHBs gene and carrier pSW3891 plasmid optimized by the codon; 10ul connection system is configured to obtain a middle protein gene. The nucleic acid vaccine of the invention overcomes the defects that the differences between prokaryote and eukaryote in terms of codon preferences cause that the foreign gene can not be expressed effectively in mammal reservoir and can not generate relatively good immune sheltering effect; in addition, the invention remarkably improve protein expression of the foreign gene in the mammal reservoir, effectively stimulates immune system of the reservoir to generate relatively good immunological reaction of human body fluids and cellular immune response.

Description

technical field [0001] The invention relates to a hepatitis B vaccine, in particular to a codon-optimized hepatitis B virus nucleic acid vaccine. Background technique [0002] Before the present invention, along with the development of science, nucleic acid vaccine was paid more and more attention to as a kind of novel vaccine gradually. At present, most of the target genes used to construct nucleic acid vaccines come from prokaryotes such as viruses or bacteria, while the research and application objects of vaccines are mainly eukaryotes, such as mice, macaques or human beings and other advanced mammals. The problem is that due to the differences in codon usage preferences between prokaryotes and eukaryotes, the foreign genes used to construct nucleic acid vaccines cannot be effectively expressed in mammalian hosts, and therefore cannot effectively stimulate the host's immune system. This is the main reason for the low immunogenicity of current nucleic acid vaccines. Con...

AI Technical Summary

Problems solved by technology

The problem is that due to the differences in codon usage preferences between prokaryotes and eukaryotes, the foreign genes used to construct nucleic acid vaccines cannot be effectively expressed in mammalian hosts, and therefore cannot effectively stimulate the host's immune system. This is the main reason for the low immunogenicity of current nucleic acid vaccines.

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