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Method for induction and subculture proliferation regeneration of banana embryogenic callus

A technology for subculture proliferation and embryogenic callus, which is applied in the field of plant tissue culture, can solve the problems of low embryogenic rate and seedling rate, low induction rate of embryogenic callus, long induction time, etc., and achieve embryonic rate High, improved induction efficiency and multiplication efficiency, and low production cost

Inactive Publication Date: 2011-12-07
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

At present, the low induction rate of embryogenic callus, long induction time, and low embryonic and seedling formation rates are the main bottlenecks affecting the wide application of this receptor system.

Method used

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Embodiment Construction

[0021] The present invention will be further described below.

[0022] A method for the induction and subculture proliferation and regeneration of banana embryogenic callus, comprising: a culture process for inducing dedifferentiated callus, a culture process for inducing fragile embryogenic callus, and a subsequent culture process for fragile embryogenic callus. Proliferation, induction of embryogenic callus into embryos, induction of mature embryos into seedlings.

[0023] 1) The culture process of induced dedifferentiation callus

[0024] Select the disease-free young sucking buds of bananas that have just been unearthed. After surface treatment, the terminal buds are stripped and cut into thin slices with a thickness of 1-2 mm and a length and width of 0.5-1 cm. They are inoculated on the dedifferentiation callus induction medium for dark culture. The white dedifferentiated callus is obtained, the culture temperature is 25-30 DEG C, and the culture time is 20-30 days. Th...

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Abstract

The invention discloses a method for the induction, subculture, proliferation and regeneration of banana embryogenic callus. The process of subculture and proliferation of sexual callus, the process of induction of embryogenic callus into embryos, and the process of induction of mature embryos into seedlings. The invention has the advantages of simple technological process, low production cost, high embryogenic tissue induction and proliferation rate, long subculture time, and overcomes low embryogenic response of explants and difficulty in embryogenic callus induction during the banana tissue culture process. The low rate of induced embryo formation and seedling formation, as well as the long induction cycle and other technical problems provide a new technical route for banana transformation technology, and also provide an efficient regeneration system for the production of juvenile tissue culture banana seedlings, which has great application value .

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for efficiently inducing banana embryogenic callus, achieving long-term subculture and proliferation through plant tissue culture technology, and inducing the subcultured embryogenic callus to form embryos and seedlings Methods. Background technique [0002] Banana is an important economic crop with both fruit and food in tropical and subtropical developing countries. As the world's fourth largest food crop after rice, wheat, and corn, the healthy development of the banana industry has an important socio-economic status. In 1985, the South China Institute of Botany, Chinese Academy of Sciences took the lead in successfully industrializing the production technology of banana tissue culture seedlings. At present, banana tissue culture technology is very mature and has been widely used in the production of banana seedlings. It has achieved remarkable economic and social be...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵辉曾会才王旭彭明
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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