Abalone HINT gene and protein and application of same for preparing anticarcinogenic drug

A protein and gene technology, applied in the fields of peptide/protein components, applications, anti-tumor drugs, etc., can solve the problems of in-depth research, limited development and utilization, etc.

Inactive Publication Date: 2012-03-14
ZHEJIANG UNIV
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of in-depth research on its corresponding functional factors, especially the lack of research on some genes that are beneficial to the treatment of human tumors, its further development and utilization are limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] The methods of molecular biology used in the following examples of the present invention are all known techniques.

[0011] Implementation steps of the present invention include:

[0012] 1. Using the total RNA of abalone hemolymphocytes as a template, construct an abalone cDNA library and obtain the complete gene nucleotide sequence of HINT.

[0013] 2. Using DNA recombination technology to clone the sequence fragment of the HINT gene coding frame into a suitable pMD18-T vector (purchased from Takara Company, Japan), and then digested with restriction endonucleases, and pET- 28(a) prokaryotic expression vector (purchased from Novagen, USA) was ligated to obtain the recombinant expression plasmid pET-28(a)-HINT.

[0014] 3. Transform the positive recombinant plasmid PET-28(a)-HINT into the expression host strain BL21 (purchased from Tiangen Company, Germany), and induce expression with isopropylthiogalactoside (IPTG) (purchased from Sangon Company, Canada) , SDS-polya...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the biological technical field and aims at providing abalone HINT gene and protein in an abalone and an application of the same for preparing an anticarcinogenic drug. The proThe invention relates to the biological technical field and aims at providing abalone HINT gene and protein in an abalone and an application of the same for preparing an anticarcinogenic drug. The protein coded by the abalone HINT gene has amino acid sequences shown in SEQ ID NO:2. The gene coding the protein has nucleotide sequences shown in SEQ ID NO:1. The invention also provides the applicatiotein coded by the abalone HINT gene has amino acid sequences shown in SEQ ID NO:2. The gene coding the protein has nucleotide sequences shown in SEQ ID NO:1. The invention also provides the application of the protein for preparing the anticarcinogenic drug. The invention obtains the gene nucleotide complete sequence of the abalone HINT, c and expresses the soluble HINT protein product through consn of the protein for preparing the anticarcinogenic drug. The invention obtains the gene nucleotide complete sequence of the abalone HINT, c and expresses the soluble HINT protein product through constructing procaryon expressing carriers. The protein has the function of promoting hepatoma cell to be apoptotic, can be used as the component for inducing the cell apoptosis and can be applied to thetructing procaryon expressing carriers. The protein has the function of promoting hepatoma cell to be apoptotic, can be used as the component for inducing the cell apoptosis and can be applied to thepreparation of the anticarcinogenic drug.preparation of the anticarcinogenic drug.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the abalone HINT gene and protein in the abalone body and the application thereof in the preparation of anticancer drugs. Background technique [0002] Histidine triad nucleotide binding protein (HINT) is a member of the histidine triad protein superfamily, a family with nucleotide transferase and hydrolase activities, crystal structure study The results showed that HINT was the ancestor of four other members of the histidine trimer protein superfamily. Since HINT was isolated in cattle and proved to have biological functions, many homologues of HINT have been cloned successively from rabbits, humans, and even plants. Studies have shown that the protein has high structural and functional similarities in lower animals and higher animals, and the conservation of structure and function indicates that HINT plays an important biological function. Research reports have shown that HINT prot...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61P35/00C12N15/55A61K38/46C12N9/22
Inventor 吴信忠吴刘记
Owner ZHEJIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap