Abalone HINT gene and protein and application of same for preparing anticarcinogenic drug

Abstract

The invention relates to the biological technical field and aims at providing abalone HINT gene and protein in an abalone and an application of the same for preparing an anticarcinogenic drug. The proThe invention relates to the biological technical field and aims at providing abalone HINT gene and protein in an abalone and an application of the same for preparing an anticarcinogenic drug. The protein coded by the abalone HINT gene has amino acid sequences shown in SEQ ID NO:2. The gene coding the protein has nucleotide sequences shown in SEQ ID NO:1. The invention also provides the applicatiotein coded by the abalone HINT gene has amino acid sequences shown in SEQ ID NO:2. The gene coding the protein has nucleotide sequences shown in SEQ ID NO:1. The invention also provides the application of the protein for preparing the anticarcinogenic drug. The invention obtains the gene nucleotide complete sequence of the abalone HINT, c and expresses the soluble HINT protein product through consn of the protein for preparing the anticarcinogenic drug. The invention obtains the gene nucleotide complete sequence of the abalone HINT, c and expresses the soluble HINT protein product through constructing procaryon expressing carriers. The protein has the function of promoting hepatoma cell to be apoptotic, can be used as the component for inducing the cell apoptosis and can be applied to thetructing procaryon expressing carriers. The protein has the function of promoting hepatoma cell to be apoptotic, can be used as the component for inducing the cell apoptosis and can be applied to thepreparation of the anticarcinogenic drug.preparation of the anticarcinogenic drug.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the abalone HINT gene and protein in the abalone body and the application thereof in the preparation of anticancer drugs. Background technique [0002] Histidine triad nucleotide binding protein (HINT) is a member of the histidine triad protein superfamily, a family with nucleotide transferase and hydrolase activities, crystal structure study The results showed that HINT was the ancestor of four other members of the histidine trimer protein superfamily. Since HINT was isolated in cattle and proved to have biological functions, many homologues of HINT have been cloned successively from rabbits, humans, and even plants. Studies have shown that the protein has high structural and functional similarities in lower animals and higher animals, and the conservation of structure and function indicates that HINT plays an important biological function. Research reports have shown that HINT prot...

AI Technical Summary

Problems solved by technology

However, due to the lack of in-depth research on its corresponding functional factors, especially the lack of research on some genes that are beneficial to the treatment of human tumors, its further development and utilization are limited.