Additive capable of reducing injection-shear-force injury to stem cell suspension and stem cell suspension

Abstract

The invention discloses an additive capable of reducing injection-shear-force injury to a stem cell suspension. The additive is characterized by being prepared from the following raw materials in parts by weight: 0.1-5 parts of hyaluronic acid, 1-5 parts of type II collagen hydrolyzate, 0.01-0.1 part of cholesterol, 0.1-2 parts of glutathione and 0.1-1 part of ceramide. The stem cell suspension isadded with the additive. According to the additive-added stem cell suspension, regardless of syringes of any specification, both the cell activity and the cell completeness are increased greatly compared with those of control groups, thus, it is described that the additive can protect cells and reduce injury caused by shear force of syringe needles, the activity of cells is further guaranteed, relatively high cell activity is maintained, the stem cell suspension can better play roles after reaching a human body, the treatment effect is improved, and then, the stem cell suspension plays a significant role during disease treatment.

Description

Technical field[0001]The present invention belongs to the field of stem cells, and in particular, therefore relating to an additive and stem cell suspension that can reduce the injection of shear for stem cell suspension.Background technique[0002]Stem cell therapy is to introduce a progenitor cell into tissue or organ, as a means for treating various diseases and injuries, using biomarns as a carrier to implant the cells into local tissue to achieve regeneration purposes. In the past four decades, due to its unique biocompatibility, chemical and physically diverse hydrogels have become standard materials for regenerative biology. Hyaluronic acid (HA) hydrogel is a synthetic biomaterial that protects the encapsulated cells from inflammation and surrounding macrophages, and provides biocompatibility for cell attachment, survival, migration, growth and proliferation. surroundings.[0003]Because of the purpose of use, we generally choose different syringe needles. Short and thick needles...

AI Technical Summary

Problems solved by technology

Short and thick needles can reduce the damage to the cells caused by the shear force of the cell suspension in the needle and improve the cell survival rate. However, due to the shallow depth of entry, stem cells will also migrate to other organs, resulting in stem cells targeting organs Insufficient concentration

Long and thin needles can better enable stem cells to reach specific parts from the entry point, but the shear force of the cell suspension in the needle will cause greater damage to the cells and will also affect the concentration of stem cells in the target organ

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