Method for cultivating high-female few-seed watermelon

A watermelon variety and cultivation method technology, applied in the directions of botanical equipment and methods, plant products, plant genetic improvement, etc., to achieve the effects of low price, improved transfer efficiency, and overcoming difficulties in fruit setting

Inactive Publication Date: 2009-09-16
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The integration of watermelon strong female lines and watermelon few-seeded breeding materials, the method of breeding strong female few-seeded watermelon varieties and the few-seeded watermelon varieties have not been reported at home and abroad

Method used

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  • Method for cultivating high-female few-seed watermelon
  • Method for cultivating high-female few-seed watermelon
  • Method for cultivating high-female few-seed watermelon

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for cultivating strong female seedless watermelon varieties, comprising the steps of:

[0022] ①Obtaining different watermelon translocation materials: a. The few-seeded fruit that appeared in the progeny of crossing the American watermelon Jubilee breeding material with "Jinhua No. 1" can be homozygous for 6 generations and test crossed for 1 generation The basic material for homozygous non-homologous chromosome translocation breeding was bred, code-named Y-5; b. Non-homologous chromosome translocation was found in the material isolated from the male degenerate offspring of watermelon. This backcross was used to breed a homozygous male sterile non-homologous chromosomal translocation material, code-named YN-10; c. Non-homologous chromosomal translocation test: Y-5 and YN-10 were crossed and observed Its F 1 The abortion rate of generation seeds and pollen showed that the abortion rate of seeds was about 75%, and the abortion rate of pollen was also about 75%....

Embodiment 2

[0028] The determination method of non-homologous chromosome translocation is as follows: ① Genetic analysis is carried out on the test cross of different seedless materials with normal seeded materials as the female parent, and the F 1 50% of the fruit set normal seeds; 50% of the fruit set half of the seeds, and the pollen abortion rate was 50%, the cytological identification was carried out, and when the chromosomes formed a "cross" junction or formed a "bridge", it was determined to be non-homologous Chromosomal translocation material; ② use known homozygous translocation material as female parent, and the non-homologous chromosomal translocation material as male parent cross, F 1 It shows that the pollen abortion rate is about 75%, and the number of seeds of a single melon is about 25% of the normal fruit, so it is determined that the tested material and the female parent are different translocations.

Embodiment 3

[0030] A method for cultivating strong female watermelon varieties with few seeds, using "Jingxin No. 1" instead of "Jinhua No. 1" in Example 1, and other materials and steps are the same as in Example 1.

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Abstract

The invention discloses a method for cultivating high-female few-seed watermelon. The method comprises the steps of obtaining different watermelon translocation materials Y-5 and YN-10, obtaining a watermelon high-female gene material H-12, performing high-female gene transfer on the Y-5 and the YN-10 taken as recurrent parents together with the H-12 by adopting the methods of generation-II back cross, generation-II self cross and generation-I test cross, hybridizing a homozygous YNC-10 high-female translocation material and a homozygous YC-5 high-female translocation material, and obtaining the high-female few-seed watermelon with objective traits through a field variety comparing-testing-breeding procedure. The method transfers different chromosome translocation to a female-parent material and a male-parent material respectively, thereby not only improving transfer efficiency but also ensuring that transfer materials are easy to protect. The watermelon cultivated by the method is few in watermelon seeds, convenient to eat, large in quantity of female flowers and easy to cultivate. In addition, due to few seeds, the method also reduces harmful effects of seed respiration on storage, thereby strengthening storage property.

Description

technical field [0001] The invention belongs to plant breeding, in particular to a method for cultivating watermelon varieties. Background technique [0002] At present, there are two methods for cultivating watermelons with few seeds. The first method is to use 4X to cultivate watermelons with few seeds: this method is to induce 2X watermelons into 4X watermelons with 0.2% colchicine, and use 4X watermelons with few seeds to produce 4X watermelons. Features Cultivate watermelons with few seeds. However, due to the shortcomings of 4X watermelons such as thick skin, late maturity, small melons, and low seed yield, only 4X watermelons with few seeds have been reported at present, and there are no popular varieties. The second method is to use chromosomal translocation to cultivate watermelons with few seeds: use radiation, chemical mutagenesis and other methods to create non-homologous chromosomal translocations, obtain heterozygous translocations, and then carry out self-cro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H5/00
Inventor 焦定量张艳宁商纪鹏黄国清郝健全
Owner TIANJIN RES INST OF VEGETABLE
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