Method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation

A technology of synergistic fermentation and Cordyceps militaris, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of improving quality, increasing yield, increasing yield and efficiency

Inactive Publication Date: 2009-09-16
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, there is no relevant report on the technology of increasing the yield of Co

Method used

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  • Method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation
  • Method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation

Examples

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Embodiment 1

[0033]Example 1: Put 25g of rice into 10 washed colorless glass bottles respectively, add 40ml of distilled water according to the ratio of material to water of 1:1.6, after sealing, sterilize by autoclaving at 121°C for 30min, and inoculate after cooling. The spore suspension of the red yeast rice (ATCC16365) strain was placed at 30°C and incubated in the dark at constant temperature. 25g of rice, 1.2g of wheat bran, 0.4g of corn flour, and 0.4g of soybean flour were respectively loaded into 90 canned bottles, and 43.2ml of nutrient solution was added at a material-water ratio of 1: 1.6 (preparation method was glucose 10g, peptone 5g, MgSO 4 □7H 2 O 1g, KH 2 PO 4 2 g, ammonium citrate 1 g, water 1000 ml); autoclave, after cooling, inoculate the spore suspension of Cordyceps militaris (CGMCC No.2459) strain, and place at 26° C. for dark constant temperature cultivation.

[0034] After the cultured red yeast rice and Cordyceps militaris are sealed, mix the rice culture of ...

Embodiment 2

[0035] Example 2: Put 20g of rice into 20 cleaned colorless glass bottles respectively, add 32ml of distilled water according to the ratio of material to water of 1:1.6, after sealing, sterilize by autoclaving at 121°C for 30 minutes, and inoculate after cooling. The spore suspension of the red yeast rice (ATCC 16365) strain was placed at 30°C and incubated in the dark at constant temperature. 20g of rice, 1g of wheat bran, 0.3g of corn flour, and 0.3g of bean flour were added to 180 canned bottles, and 34.6ml of nutrient solution was added according to the ratio of material to water of 1:1.6 (the preparation method was 10g of glucose, 5g of peptone, MgSO 4 ·7H 2 O 1g, KH 2 PO 4 2 g, ammonium citrate 1 g, water 1000 ml); autoclave, after cooling, inoculate the spore suspension of Cordyceps militaris (CGMCC No.2459) strain, and place at 26° C. for dark constant temperature cultivation.

[0036] After the cultured red yeast rice and Cordyceps militaris are sealed, mix the ri...

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Abstract

The invention discloses a method for improving output of cordyceps militars fruiting body and cordycepin by adopting red yeast rice synergistic fermentation, wherein bacteria strain activation and antagonism test are required beforehand, and afterwards, the cordycepin are required to be detected. The method comprises four stages: (1) red yeast rice cultivation; (2) cordyceps militars cultivation; (3) dixenie mixed synergistic fermentation; and (4) fruiting body management. The method not only can significantly improve the output of the cordyceps militars fruiting body by more than 30 percent, but also can improve the quality of the cordyceps militars fruiting body, thus causing the content of cordycepin in the fruiting body to be improved by 20 percent and reach more than 20.2mg/g. The method has great significance in improving the output and efficiency of cordyceps militars solid fermentation, and improving the content of effective constituents in medical cordycepin powder and capsule, thus having good application and development prospect.

Description

technical field [0001] The invention relates to fungal fermentation engineering, in particular to solid culture of Cordyceps militaris and production of secondary metabolites thereof. Background technique [0002] Cordyceps militaris (L.) Link], also known as Cordyceps militaris, belongs to the kingdom of fungi, phylum Ascomycota, class Ascomycetes, subclass Faecalis, Hypocreales, Ergotaceae, and the genus Cordyceps. Cordyceps militaris. It is the model species of the fungus of the genus Cordyceps, and its hosts are mainly the Lepidoptera Boriidae, Namphidae, Cephaliidae and so on. It is a complex composed of two parts, the sub-seat and the sclerotia. Its anamorph is Paecilomyces militaris (Kob.) Br. & Sm.]. It is widely distributed and is found in my country's Hebei, Shaanxi, Anhui, Jilin, Liaoning, Yunnan, Qinghai, Guizhou, Sichuan, and Guangxi. Cordyceps militaris contains a variety of active ingredients, including cordycepic acid (D-mannitol), Cordyceps polysaccharid...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/40C12R1/645
Inventor 康冀川文庭池雷帮星谢红艳
Owner GUIZHOU UNIV
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