Klebsiella pneumoniae and method of preparing hydrogen and 2, 3-butanediol thereby

A Klebsiella and butanediol technology, applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve problems such as environmental pollution, energy shortage, and urgency, and achieve high conversion efficiency and reaction conditions. Simple and easy, high production value-added effect

Inactive Publication Date: 2009-09-16
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For a long time, the excessive dependence on non-renewable resources such as petroleum has led to serious environmental pollution and energy shortages, so the task of finding alternative energy sources is urgent

Method used

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  • Klebsiella pneumoniae and method of preparing hydrogen and 2, 3-butanediol thereby

Examples

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Effect test

Embodiment 1

[0043] Method for preparing hydrogen and 2,3-butanediol using Klebsiella pneumoniae ECU-21

[0044] The formula of the fermentation medium is: glucose 10g / L, yeast powder 5g / L, peptone 10g / L, beef extract 6g / L, NaCl 5g / L, KH 2 PO 4 0.05g / L, K 2 HPO 4 0.05g / L, pH6.0~6.2; high temperature sterilization at 121℃ for 20min.

[0045] Take the slant strains stored at 4°C, pick a ring and inoculate it into a Erlenmeyer shaker flask containing 30ml of seed medium, and culture it statically at 37°C for 12 hours, then inoculate the seed medium with 5% inoculum to 600ml In the fermentation medium, the fermentation shaker flask was sealed with a rubber stopper, and then N 2Purge to form an anaerobic environment in the reactor, excess gas is discharged from the exhaust port, N 2 After the purge is completed, the air bag is used to connect the exhaust port. The hydrogen generated by the fermentation is collected by the air bag, and samples are taken from the sampling port at regular i...

Embodiment 2

[0047] Method for preparing hydrogen and 2,3-butanediol using Klebsiella pneumoniae ECU-21

[0048] Fermentation medium formula: glucose 30g / L, yeast powder 5g / L, peptone 10g / L, beef extract 6g / L, NaCl 5g / L, phosphate buffer 200mM, adjust different pH to 5.0~7.5, 121℃ High temperature sterilization for 20min.

[0049] Take the slant strains stored at 4°C, pick one ring and inoculate them into the Erlenmeyer shaker flasks containing 30ml of medium, culture them at 37°C for 12 hours, and transfer them to 600ml flasks according to the inoculation amount of 5%. , in the fermentation media with different pH, use rubber stopper to seal the fermentation shake flask, pass N 2 The air in it is exhausted to form an anaerobic environment, and the excess gas is discharged from the exhaust port, N 2 After purging, a gas bag was used to connect the exhaust port to collect the hydrogen produced by fermentation. The culture temperature was kept at 37°C, and a magnetic stirrer was used to st...

Embodiment 3

[0053] Method for preparing hydrogen and 2,3-butanediol using Klebsiella pneumoniae ECU-21

[0054] The fermentation medium is the hydrolyzate obtained by enzymatic hydrolysis of corn stalks after steam expansion pretreatment. The main composition of the hydrolyzate is: glucose 60g / L; xylose 12g / L; cellobiose 4g / L; the hydrolyzate is diluted 6 times, Add yeast powder 5g / L, peptone 10g / L, beef extract 6g / L, NaCl 5g / L, phosphate buffer 200mM, pH 6.0-6.2, and sterilize at 115°C for 20min.

[0055] The seed culture medium is the same as that in Example 1. Get the slant strains preserved at 4°C, pick a ring and inoculate it into a triangular shaker flask with 30ml of seed culture medium, and cultivate it statically at 37°C for 12 hours, and inoculate at 5%. The amount was transferred to a 1L fermentation shaker flask filled with 600ml of fermentation medium, sealed with a rubber stopper, and passed through N 2 Exhaust the air in it, and the excess gas is discharged from the exhaus...

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Abstract

The invention relates to Klebsiella pneumoniae and a method of preparing hydrogen and 2, 3-butanediol thereby. The Klebsiella pneumoniae is Klebsiella pneumoniae ECU-21 with the preservation number of CGMCC No. 2850. In the preparation method, a ring colony taken from a panel is inoculated to a seed culture medium, the inoculated seed culture medium is arranged in an incubator of 37 DEG C for the static culture, and the seed culture medium which is cultured for 12 hours is switched to a fermentation medium for the fermentation culture; after the inoculation, a rubber stopper is used for sealing a fermentation shake flask, the anaerobic culture environment is formed by introduced N2 sweeping, superfluous gas is discharged via an exhaust port, a gas bag is used to be connected with the exhaust port for collecting hydrogen produced during the fermentation after the gas discharge is finished, and the 2, 3-butanediol is obtained in liquid product; the culturing temperature is 37 DEG C, a magnetic stirrer is adopted for stirring, the rotation speed is 150rpm, and the culturing time is 12-15h. The invention has the advantages that the hydrogen and the 2, 3-butanediol are produced by the fermentation under the anaerobic condition, the additional value of the production is increased, the reaction conditions are easy and wide industrial application development prospect is provided.

Description

【Technical field】 [0001] The present invention relates to the technical field of Klebsiella pneumoniae, specifically, a Klebsiella pneumoniae and a method for preparing hydrogen and 2,3-butanediol thereof, the bacterial species involved being Klebsiella pneumoniaeECU-21, The deposit number is CGMCC No.2850. 【Background technique】 [0002] Energy is one of the material bases on which human beings depend and is the driving force for the evolution of the earth and all things. It is closely related to economic development and human progress. With the development of society and economy, human beings' demand for energy and raw materials for chemical industrial products is also increasing. For a long time, the excessive dependence on non-renewable resources such as petroleum has caused serious environmental pollution and energy shortages, so it is urgent to find alternative energy sources. As we all know, hydrogen energy has the advantages of cleanness, high efficiency, and no po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P3/00C12P7/18C12R1/22
Inventor 张旭牛坤谭文松
Owner EAST CHINA UNIV OF SCI & TECH
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