Tree peony callus induction method

A callus induction and callus technology, which is applied in the field of callus culture, can solve the problems of difficult domestication and transplanting, low rooting rate and quality, browning and the like, and achieves the effect of high callus induction rate.

Inactive Publication Date: 2009-10-07
INST OF FORESTRY CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

[0006] There are problems such as difficulty in callus induction, low rooting rate and quality, difficulty in acclimatization and transplanting, browning, vitrification, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment

[0017] Taking different explants as an example, the best induction effect was achieved by using different media and different culture conditions. In the following, different explants are used as objects to elaborate respectively:

[0018] Induction of embryonic callus:

[0019] Cultivation period: every September, after the seeds are formed;

[0020] Pre-cultivation treatment: use a combination of low temperature (4°C) treatment and gibberellin treatment, and use conventional disinfection methods (2% NaClO for 30 minutes, 70% alcohol for 30 seconds, rinse with sterile water for 3-5 times) after disinfection vaccination;

[0021] The best medium: MS+NAA0.1mg / L+6-BA1.0mg / L;

[0022] The callus induction rate reached 100%.

[0023] Stem segment callus induction:

[0024] Material collection period: take young stems, in late March and early April in spring, or young stems formed by germination of seeds;

[0025] Pre-cultivation treatment: use conventional sterilization metho...

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Abstract

The present invention discloses a tree peony callus induction method, applying MS culture medium that contains 6-BA, cytokinin, NAA plant growth regulator, 2, 4-D weedicide to induce callus. There are specific culture mediums and hormone combinations in allusion to different explants of tree peony seeds, stem, leaf, petal, anther etc. The invention also expatiates the optimal material drawn period of the callus. The inductivity of the tree peony callus are more than 80% and achieves a preferable induction impression; and the inductivity of the petal and the anther also reaches 50%.

Description

technical field [0001] The invention relates to a method for culturing callus, in particular to a method for inducing callus of peony. Background technique [0002] Peony is one of the top ten famous flowers in China, and it is also the national flower of China. It has the reputation of "the king of flowers". Tissue culture of tree peony mainly uses buds to induce plants and explants to induce plants through callus, and the callus induction of tree peony is an important stage for plant regeneration, and a higher callus induction rate is more important for later callus. How to obtain a higher callus induction rate is closely related to the type of medium, the type and concentration of hormones, and the culture conditions. [0003] Callus culture is the most common form of culture. Except for shoot tip meristem culture and part of organ culture, several other forms of plant tissue culture must eventually undergo callus to produce plants. In addition, callus is often the sour...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王雁朱向涛
Owner INST OF FORESTRY CHINESE ACAD OF FORESTRY
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