Technique for extracting polysaccharide in glossy ganoderma mycelium cell and method thereof

A technology of Ganoderma lucidum mycelium and intracellular polysaccharide, which is applied in the directions of antitoxin, metabolic disease, antitumor drug, etc., can solve the problems of increasing production cost, difficulty in product separation, etc., so as to improve the yield, improve the separation efficiency, and shorten the extraction time. effect of time

Inactive Publication Date: 2009-10-21
ZHEJIANG UNIV
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  • Abstract
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Problems solved by technology

However, since the enzyme extraction will increase the production cost and also bring difficulties to the separation of the product, the amount of addition should be strictly controlled

Method used

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  • Technique for extracting polysaccharide in glossy ganoderma mycelium cell and method thereof
  • Technique for extracting polysaccharide in glossy ganoderma mycelium cell and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Ganoderma lucidum Chizhi No. 22 bacterial strain (provided by Zhejiang Academy of Forestry Sciences) mycelium submerged fermentation mycelium dry powder polysaccharide extraction process combination, its composition is that the ratio of material to liquid is 1: 30 by mass ratio, and the addition of compound enzyme is 2%, the ultrasonic power is 200W, the enzymolysis time is 50min, the enzymolysis temperature is 55°C, and the enzymolysis pH value is 5.0.

[0023] The method for determining the intracellular polysaccharide extraction process of the ganoderma lucidum mycelia is:

[0024] 1) The mycelium dry powder obtained from the submerged fermentation of the mycelium of Ganoderma lucidum Chizhi No. 22 strain (provided by Zhejiang Academy of Forestry Sciences) was selected.

[0025] 2) Determine the ratio of material to liquid

[0026] Polysaccharides were extracted by hot water extraction. Select 100g of mycelium dry powder from step 1), take the water addition multip...

Embodiment 2

[0035] Ganoderma lucidum Chizhi No. 38 strain (provided by Zhejiang Academy of Forestry Sciences) mycelia dry powder polysaccharide extraction process combination obtained from mycelium submerged fermentation, its composition is that the ratio of solid to liquid is 1:40 by mass, and the amount of enzyme added is 3.0% Under the conditions, the ultrasonic power is 150W, the enzymolysis time is 40min, the enzymolysis temperature is 45°C, and the enzymolysis pH value is 7.0.

[0036] The method for determining the intracellular polysaccharide extraction process of the ganoderma lucidum mycelia is:

[0037] 1) Mycelium dry powder obtained from mycelium submerged fermentation of Ganoderma lucidum Chizhi No. 38 strain (provided by Zhejiang Academy of Forestry Sciences)

[0038] 2) Determine the ratio of material to liquid

[0039] Polysaccharides were extracted by hot water extraction. Select 100g of mycelium dry powder from step 1), take the water addition multiple as the analysis f...

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Abstract

The invention relates to a technique combination for extracting polysaccharide in glossy ganoderma mycelium cell. According to the mass ratio, the solid-liquid ratio is 1:10-1:60, the adding quantity of complex enzyme is 0.5-5.0% of the mass of raw materials, the range of ultrasonic wave power is within 100-500W, the range of enzymolysis time is 40-100min, the range of enzymolysis temperature is 30-60 DEG C, and the pH value range of enzymolysis is 4.0-8.0. The technique for extracting polysaccharide in glossy ganoderma mycelium cell and the method thereof comprise: high quality mycelium is selected for submerged fermentation to obtain mycelium dry powder; the solid-liquid ratio is determined; the adding quantity of the complex enzyme is determined; and the optimum extraction technique combination for extracting the mycelium dry powder polysaccharide by using an ultrasonic wave-composite enzymolysis method is determined.

Description

technical field [0001] The invention relates to biopharmaceutical production technology, in particular to a process for extracting intracellular polysaccharides from Ganoderma lucidum mycelia and a method for determining the same Background technique [0002] Ganoderma lucidum is a traditional Chinese medicine in my country. In traditional Chinese medicine, it has the functions of nourishing and strengthening, strengthening the body and strengthening the body. It has been used for more than 2,000 years. Scientific research believes that Ganoderma lucidum has multiple functions such as two-way immune regulation, lowering blood fat, anti-aging and anti-tumor. These functions come from a variety of biologically active substances in Ganoderma lucidum, and Ganoderma lucidum polysaccharide is the main component. [0003] Ganoderma lucidum polysaccharides are widely used in medicine, food and other fields, and the market demand is increasing day by day. However, at present, the pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/02A61P3/06A61P39/06A61P35/00
Inventor 李卫旗
Owner ZHEJIANG UNIV
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