Micro-fluidic chip with an integrated PDMS surface tension minipump and application thereof

A microfluidic chip and surface tension technology, applied in instruments, biological testing, analytical materials, etc., can solve the problems of high cost, cumbersome production methods, and restricted applications, so as to improve the ability to store waste liquid and increase the maximum sample injection volume effect

Inactive Publication Date: 2009-10-21
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the surface tension micropumps reported in the current literature are all based on rigid materials such as silicon wafers and ceramics (David Juncker, Heinz Schmid, Ute Drechsler, Heiko Wolf, Marc Wolf, Bruno Michel, Nico deRooij, and Emmanuel Delamarche, 2002. Autonomous Microfluidic CapillarySystem.Anal.Chem.74:6139-6144; Martin Zimmermann, Heinz Schmid, Patrick Hunzikerb and Emmanuel Delamarche.2007.Capillary pumps for autonomous capillary systems.Lab on a Chip.7:119-125.), the production method is cumbersome , the cost is high, thus restricting its application on microfluidic chips

Method used

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  • Micro-fluidic chip with an integrated PDMS surface tension minipump and application thereof
  • Micro-fluidic chip with an integrated PDMS surface tension minipump and application thereof
  • Micro-fluidic chip with an integrated PDMS surface tension minipump and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The microfluidic chip surface tension micropump made based on PDMS realizes the adjustment of different flow rates. chip such as Figure 4 As shown in the middle *, PDMS is used to cover the outlet of the waste liquid pool of the micropump to control the number of waste liquid pools, so that the linear adjustment of the flow rate can be realized within the flow rate scale of nL / s.

[0024] When the number of outlets of the waste liquid pool is zero, the solution stops flowing in the channel at this time, and the flow rate is zero.

Embodiment 2

[0026] Microfluidic chip surface tension micropump immunoassay system based on PDMS. The whole immunoassay process requires antibody solution, bovine serum albumin solution, washing solution, sample solution and labeled antibody solution. Use a pipette gun to add these solutions into the sample solution pool in sequence according to the order of the immune reaction. These solutions will flow through the reaction channel in sequence under the action of surface tension, and each step of the immune reaction can be automatically completed, such as coating, sealing, etc. , reaction, washing, etc.

[0027] The whole immunoassay process (using human IgG as a model analyte) is described as follows:

[0028] 1. Use a pipette gun to add the goat anti-human IgG solution into the sample liquid pool. Under the action of surface tension, it will gradually flow into the reaction channel. Since the material used in the reaction channel is unmodified PDMS, which is hydrophobic, the antibody ...

Embodiment 3

[0039] The microfluidic chip surface tension micropump multi-index immunoassay system based on PDMS, the results are as follows Image 6 shown. Both the upper and lower layers of the chip are PDMS. The upper layer of PDMS is a surface tension micropump, which provides fluid transport power, and the lower layer of PDMS is unmodified, providing a carrier for immune response. The lower layer of PDMS is pre-coated with different antibody bands, and the mixture of samples and various detection antibodies is sequentially added to the sample liquid pool to realize simultaneous analysis and detection of multiple indicators of a sample.

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Abstract

The invention provides a micro-fluidic chip with an integrated PDMS surface tension minipump and an application in immunoassay. The chip comprises a sample-adding liquid bath, a mixing unit, a reaction passage, a flow speed control unit, a PDMS surface tension minipump and a waste liquid bath which are all arranged on the same PDMS chip encapsulated in another layer of PDMS or glass. The micro-fluidic chip utilizes the PDMS with an added emulsifier or nonionic surface activator to manufacture and integrate the low-cost surface tension minipump, fully integrates a reaction unit and a driving unit on the chip, does not need software control, external interfaces and external power sources, is suitable for field analysis and has the advantages of small size, low cost, extremely simple operation, and the like.

Description

technical field [0001] The invention relates to a microfluidic chip integrated with a fluid-driven micropump and an application thereof, and in particular provides a microfluidic chip integrated with a PDMS surface tension micropump and an application thereof. Background technique [0002] Microfluidics (Lab-on-a-chip) is a new interdisciplinary field, which refers to the integration or basic integration of basic operating units such as sample preparation, reaction, separation, and detection involved in the biochemical field. On a chip of a few square centimeters (or even smaller), a network of microchannels is formed, and a controllable fluid runs through the entire system to replace various functions of conventional biological or chemical laboratories. It is an important part of micro-nano technology and one of the main technical platforms for systems biology research. [0003] In order to realize various researches and applications on the platform of the lab-on-a-chip, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N35/00G01N33/53
Inventor 秦建华陆瑶姜雷林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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