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An analysis method and electrode technology, which are applied in the field of amperometric analysis of ppt-level Hg2+, can solve the problems of low detection limit, low sensitivity, and no electrochemical detection of heavy metal ions, etc., and achieve the effects of high detection sensitivity and low cost.
Active Publication Date: 2019-05-17
HUNAN NORMAL UNIVERSITY
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Although there are many studies on the use of enzyme-catalyzed metal deposition for signal amplification, there are no reports on the electrochemical detection of heavy metal ions using this method.
In addition, the existing common methods for direct detection of soluble enzymatic products have diffusion effects, which have the disadvantages of high detection limit and low sensitivity.
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[0034] Example: Hg 2+ Electroanalysis
[0035] This embodiment is for the heavy metal ion Hg 2+ A method for quantitative analysis, comparing the analytical performance of the present invention with conventional methods.
[0036] This embodiment includes the following steps:
[0037] (1) The inventive method: in the prepared Strep-ALP / BSA / P2 / Hg 2+ / MCH / P1 / Au plate / GCE electrode for enzymatic metal deposition reaction, simply put 10 μL of 1 mmol / L AgNO on the electrode prepared above 3 ,1.5 mmol / L sodium ascorbyl phosphate (SAP) and 4 mmol / L NH 3 ·H 2 O (Join NH 3 ·H 2 O prevents AgNO 3 and SAP to form insoluble matter) enzymatic reaction solution, dark reaction at 37 ℃ for 20 min, to obtain the electrode surface rich in metallic silver (Ag / Strep-ALP / BSA / P2 / Hg 2+ / MCH / P1 / Au plate / GCE); the final electrode was washed three times with ultrapure water and dried naturally before use.
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Abstract
A ppt level Hg<2+> ampere analysis method includes steps: (1) taking an enzyme labeled DNA electrode as a working electrode, and centering on enzyme to perform enzymatic metal deposition reaction on the working electrode, namely adding enzymatic reaction liquid on the enzyme labeled DNA electrode to obtain an electrode surface rich in elementary metal after reaction; (2) applying the electrode surface rich in elementary metal to Hg<2+> electrochemical analysis, adopting an in-situ anodic stripping voltammetry method for acquiring stripping voltammetry signals of metals enriched on the working electrode, and accordingly quantitative analysis of Hg<2+> in samples can be indirectly realized, and ppt level Hg<2+> can be detected according to an electrochemical method. By quantitative analysis of heavy metal ions Hg<2+>, high detection sensitivity, low cost, simplicity, convenience, suitableness for field in-situ analysis and the like can be realized, and ppt level Hg<2+> detection can be realized as well.
Description
technical field [0001] The present invention relates to a ppt grade Hg 2+ The amperometric analysis method, specifically involves a T-Hg-based 2+ -T interaction and enzymatically deposited metal amplified signal in ppt Hg 2+ Amperometric analysis method. Background technique [0002] Mercury, one of the most toxic heavy metal elements, can cause a variety of health problems in people of all ages, such as brain damage, kidney failure, and varying degrees of cognitive and motor impairment [Ariya, P. A.; Amyot, M.; Dastoor, A.;Deeds, D.; Feinberg, A.; Kos, G.; Poulain, A.; Ryjkov, A.; Semeniuk, K.;Subir, M.; Toyota, K. Chem. Rev. 2015, 115, 3760]. The World Health Organization (WHO) and the US Environmental Protection Agency (EPA) stipulate that the mercury content in drinking water should not exceed 1 μg / L (1 ppb) and 2 μg / L (2 ppb) respectively [Aragay, G.; Pons, J. ; Merkocci, A. Chem. Rev. 2011, 111, 3433]. According to the "Surface Water Environmental Quality Stand...
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