A portable absorbance measuring device and method thereof

Abstract

The present disclosure provides a portable absorbance measuring device and method thereof, which includes a cassette, an excitation light source is arranged in the cassette, a three-channel measurement pool is provided on the emission path of the light source, and an optical extinguisher is provided at the other end of the measurement pool; the three-channel The measurement cell includes two fluorescence measurement cells and an absorbance measurement cell in the middle for adding sample solution. The fluorescence images of the two fluorescence measurement cells under the excitation of the light source are taken by the shooting tool, and the captured image is measured by the controller for image brightness , non-linear correction, absorbance and concentration calculation data processing process, the absorbance of the sample solution is obtained through the image brightness ratio of the fluorescence measuring pool on both sides of the absorbance measuring pool.

Description

technical field [0001] The present disclosure relates to a portable absorbance measuring device and a method thereof. Background technique [0002] The statements in this section merely provide background information related to the present disclosure and do not necessarily constitute prior art. [0003] Colorimetric analysis is a commonly used detection method, which is widely used in on-site analysis and determination. It usually uses natural light (such as sunlight) as the lighting source, and uses visual methods to compare the color of the analyte itself or the product after reacting with a chromogenic reagent. Color, compared with the standard concentration color scale, for rapid determination. Due to the limitation of the change of the intensity of the external light source and the large subjective error in the judgment of the human eye on the color, the measurement result is usually semi-quantitative. The monochromatic light source is used to replace the natural ligh...

AI Technical Summary

Problems solved by technology

In terms of absorbance measurement, Hussain et al. used the lighting of the smartphone as the light source, cooperated with the optical filter to split the light, and used the transmission of the optical fiber to make the monochromatic light pass through the colorimetric cell, and measured the change of light intensity with the exposure sensor in the mobile phone. ( image 3 ), using the principle that fluoride ions decolorize the colored complexes of zirconium to measure fluoride ions in drinking water (I. Hussain, et al, Anal. Chem., 2017, 89, 767–775), but the signal measured by this device is only It is transmitted light intensity rather than absorbance, and has a nonlinear relationship with fluorine ions. In addition, the change of light source intensity directly reflects the change of signal, which seriously affects the accuracy of the measurement results.

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