Patterned biochemical analysis test paper and manufacturing method and application thereof

A technology for biochemical analysis and production methods, applied in chemical instruments and methods, biological testing, layered products, etc., can solve the problems of unfavorable patterned biochemical analysis test paper application, cumbersome production methods, high cost, etc., and achieve low cost and high production cost. Fast, easy-to-use effects

Inactive Publication Date: 2012-10-10
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0004] At present, the method of making patterned biochemical analysis test paper reported in the literature mainly uses materials such as photoresist SU8 or polydimethylsiloxane (PDMS), which is expensive and uncommon, and the production method is relatively cumbersome, which is not conducive to patterned biochemical analysis. Application of analytical test paper (Patterned Paper as a Platform for Inexpensive, Low-Volume, Portable Bioassays Andres W.Martinez, Scott T.Phillips, Manish J.Butte, and George M.Whitesides Angew.Chem.Int.Ed.2007,46 , 1318–1320; Low-Cost Printing of Poly(dimethylsiloxane) Barriers To Define Microchannels in Paper, Analytical Chemistry Published on Web 03 / 12 / 2008)

Method used

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  • Patterned biochemical analysis test paper and manufacturing method and application thereof
  • Patterned biochemical analysis test paper and manufacturing method and application thereof
  • Patterned biochemical analysis test paper and manufacturing method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Patterned test paper made by three different methods, the three methods mainly include:

[0021] (1) Use a pencil to draw the required pattern on the absorbent paper, and then use a crayon to draw along the pencil line (the front and back of the absorbent paper must be drawn with a crayon), and put the drawn wax paper into a high-temperature container (such as Oven, the temperature is slightly higher than the softening temperature of the wax used) for about a few minutes (the time depends on the temperature conditions used and the wax material used). In this way, the wax will soften into a liquid and be absorbed by the absorbent paper, which will block the fibers of the absorbent paper and form various millimeter-scale hydrophilic channels. After cooling, add reagents to each reaction area to become a multi-index biochemical analysis test paper (see figure 2 ).

[0022] (2) Use drawing software to design the required pattern on the computer, then use an inkjet printer...

Embodiment 2

[0026] Use patterned test paper to detect BSA (bovine serum albumin) and glucose at the same time. The specific implementation process is as follows: For BSA detection: first add 0.5 μL of 0.3M citrate buffer to the outer circle of the three channels of the test paper (pH=1.8), and then add 0.5 μL of bromophenol blue solution (3.3 mM ethanol solution); for glucose detection: first drop 0.5 μL of potassium iodide solution (0.6 M), and then dropwise add 0.5 μL of a solution of horseradish peroxidase and glucose oxidase on top. After the above solution is dried in the air, add the mixed solution of glucose and BSA at the central sample addition point, the sample will automatically flow to each branch, and react with the above dry reagent and develop color. Among them, BSA will react with bromophenol blue under acidic conditions to obtain a blue product, and glucose will react with potassium iodide under the catalytic reaction of glucose oxidase and horseradish peroxidase to produ...

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Abstract

The invention relates to a patterned biochemical analysis test paper and a manufacturing method and application thereof. The test paper is a layered structure formed by wax and absorbent paper, wherein the first layer and the third layer are wax, and the second layer is absorbent paper; the manufacturing method of the patterned biochemical analysis test paper comprises the following steps: the required patterns are designed on the absorbent paper through a manufacturing tool, then the lines of the patterns at one side and the other side of the absorbent paper are described with the wax, the well described wax paper is put in a high temperature vessel, the wax is softened into liquid to be absorbed by the absorbent paper to form a plurality of millimetre-sized hydrophilic channels, and a biochemical analytical reagent is added in the reaction area after the cooling to obtain the patterned biochemical analysis test paper; the patterned biochemical analysis test paper is applied to the multi-index biochemical analysis for various fluid samples such as urine, blood, saliva, water, and the like; and the manufacturing method has the advantages of high speed, low cost, simple operation, and the like.

Description

technical field [0001] The invention relates to the development and application of biochemical analysis products, and in particular provides a patterned biochemical analysis test paper, its manufacturing method and application. Background technique [0002] As a convenient and fast analysis method, test strips have been widely used in real life, such as common pH test strips, urine test strips (quadruple, octagon, etc.). Compared with the traditional production method of various biochemical analysis test papers, the present invention is one-time forming, convenient and fast, and low in cost, and is very suitable for the production of multiple biochemical analysis test papers at one time. This test paper is also called a microfluidic chip on paper. . [0003] Microfluidics (Lab-on-a-chip) is a new interdisciplinary field, which refers to the integration or basic integration of basic operating units such as sample preparation, reaction, separation, and detection involved in t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/52B32B37/00
Inventor 秦建华陆瑶姜雷林炳承
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
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