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Standard for determining spewing potential of grains and preparation method thereof

A standard product and potential technology, applied in the field of grain gushing potential standard product detection, can solve problems such as inability to truly and effectively reflect grain gushing potential, lack of literature reports, etc., and achieve the effects of easy operation, strong standardization and simple process conditions

Active Publication Date: 2012-07-25
中粮麦芽(大连)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In many cases, red wheat kernels are not all caused by Fusarium contamination, and lightly polluted red wheat kernels are not easily observed by the naked eye, so this method cannot truly and effectively reflect the gushing potential of grains
[0003] If you want to obtain an objective test result, when testing the gushing potential of grains, you need standard gushing malt, and then use standard gushing malt for gushing detection, so that the gushing amount of beer is between 50g-160g, but there is no literature report yet. Related Standards

Method used

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  • Standard for determining spewing potential of grains and preparation method thereof
  • Standard for determining spewing potential of grains and preparation method thereof
  • Standard for determining spewing potential of grains and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The standard substance for determining the gushing potential of grain is to inoculate the aseptic malt with Fusarium mold to produce gushing factor, and make the beer gushing amount reach 50-160g.

[0019] The preparation method of the standard substance is specifically as follows:

[0020] Take Australian malt, take 5KGY| Co-60 Obtain sterile malt after ray irradiation; put 2 pieces of filter paper in a plate, put 10g of sterile malt after sterilization, and inoculate the sterile malt with a concentration of 10 4 5mL of Fusarium spore suspension about 1 / mL; cultivated in a biochemical incubator at 15°C for 8 days to obtain Fusarium-contaminated malt (such as figure 2 ); use the fusarium-contaminated malt cultivated in the plate as the inoculum, and amplify the inoculation to micro-malting. The micro-malting equipment is the Joe White brand produced in Australia, and the micro-malting amount is 8kg. The temperature is 15°C, cultured for 8 days, the humidity is kept a...

Embodiment 2

[0022] The preparation method described in Example 1 is used to prepare and measure the grain gushing potential standard substance, and the irradiation dose is changed to 10KGY, and the inoculum concentration of 10g sterile malt is 10 5 Individual / mL Fusarium spore suspension 10ml, obtain Fusarium-contaminated malt (such as image 3 ); The gushes of the prepared standard malt were 96g and 108g respectively.

Embodiment 3

[0024] The preparation method described in Example 1 is used to prepare and measure the grain gushing potential standard substance, and the irradiation dose is changed to 8KGY, and the inoculum concentration of 10g sterile malt is 10 4 Individual / mL of Fusarium spore suspension 8ml, the gushing amounts of standard malt prepared by testing were 96g and 108g respectively.

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Abstract

The invention relates to a standard for determining the spewing potential of grains. The standard for determining the spewing potential of the grains uses sterile malt to exclusively graft mildew, Fusarium to produce spewing factors, and ensures that the spewing amount of beer reaches between 50 and 160g. Compared with the conventional method, the method has the remarkable characteristics of providing the normal standard for determining the spewing potential of the grains, providing a reliable basis for the automatic detection of the spewing potential, ensuring that the product has steady performance, and having high detection accuracy. In addition, the standard has the advantages of reasonable preparation method, simple process conditions, and easy operation. The standard is applicable tomass industrialized production and meets detection requirements.

Description

technical field [0001] The invention relates to a standard product for detecting gushing potential of grain, and also relates to a preparation method thereof. Background technique [0002] In beer production, if mold-infected malt is used to brew beer, the produced beer may gush. The gushing of beer refers to that after the packaged beer (bottled or canned) is opened, the sudden burst of carbon dioxide causes a large amount of very fine foam to overflow, causing the foamy beer to flow out of the container. At present, it is reported that the worst factor causing gushing is the metabolite of Fusarium mold, and the detection of barley or malt gushing potential is determined by measuring the proportion of grains infected by Fusarium mold in a batch of barley or malt, The leaf buds on the back of wheat kernels that are usually infected by mold and produce gushing factors will show a special red stripe shape, and the gushing potential of the grain is determined by detecting the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/02
Inventor 邱然石殿瑜徐玉娟徐凯
Owner 中粮麦芽(大连)有限公司
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