Salbutamol artificial antigen and preparation method and application thereof

A technology of albuterol and artificial antigen, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human protein, etc. It can solve the problems of economic loss, trade obstruction, and difficulty in reaching developed countries, achieving good repeatability and easy operation. , low-cost effect

Inactive Publication Date: 2009-12-09
QINGDAO VLAND BIOTECH INC
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  • Claims
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Problems solved by technology

However, driven by economic interests, the phenomenon of illegal use in animal husbandry still exists, resulting in excessively high residues of banned drugs in some animal products, making it difficult to meet t

Method used

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  • Salbutamol artificial antigen and preparation method and application thereof
  • Salbutamol artificial antigen and preparation method and application thereof
  • Salbutamol artificial antigen and preparation method and application thereof

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Embodiment Construction

[0011] The artificial antigen of salbutamol of the present invention is formed by coupling salbutamol and carrier protein bovine serum albumin or ovalbumin, and its structural formula is

[0012]

[0013] In the formula: n is the number of molecules of salbutamol combined with a bovine serum albumin or ovalbumin molecule from 1 to 15, preferably an integer of 1 to 10; protein is bovine serum albumin or ovalbumin. The molecular weight range of Cationized Bovine Serum Album is 6.6KDa~6.9Kda, and the preferred molecular weight range is 6.7~6.8Kda.

[0014] The above artificial antigens show the following characteristics: (1) Appearance: light yellow solid powder; (2) Ultraviolet absorption wavelength: 278nm, 336nm.

[0015] When preparing the above-mentioned salbutamol artificial antigen, first connect salbutamol and activated p-aminobenric acid to form a salbutamol active intermediate with carboxyl group, and then use ethyl[3-(dimethylamino)propyl]carbodiimide as As a crosslinking...

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Abstract

The invention relates to a salbutamol artificial antigen and a preparation method and an application thereof. The preparation method comprises the following steps: linking salbutamol with activated para aminobenzoic acid to prepare salbutamol active intermediate containing carboxyl, adopting ethyl[3-(dimethylamino)propyl]carbodiimide as crosslinker to couple the methyloic salbutamol active intermediate with the amino of carrier protein, and dialyzing and freeze drying the product to obtain the antigen. The salbutamol artificial antigen can be used to prepare salbutamol polyclonal antibody. By the preparation method, the absolute immunogen of specific antibody which can prompt an immune response in animals can be prepared. The preparation method of the invention has the advantages of low cost of preparation method, simple operation and good repeatability.

Description

Technical field [0001] The invention relates to an artificial antigen of beta-agonist and a preparation method thereof, in particular to a salbutamol (salbutamol) artificial antigen and a preparation method and application thereof. Background technique [0002] Salbutamol is one of the β-stimulant drugs. It is well known that β-stimulants affect the flow and distribution of nutrients in animals, promote muscle tissue growth, reduce carcass fat content, increase lean meat rate and increase lean meat production The role of. However, β-agonists have a chemical structure and mechanism of action similar to adrenaline, and can bind to β-receptors on the cell surface to cause toxic side effects on internal organs such as liver and kidney of humans and animals. In the 1890s, there were many incidents of poisoning due to the consumption of β-stimulant drugs in various parts of the world, which caused people to attach great importance to the safety of β-stimulant drugs. At present, Europea...

Claims

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Application Information

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IPC IPC(8): C07K14/765C07K14/77C07K1/113C07K16/44
Inventor 孙海新凌红丽郗日沫
Owner QINGDAO VLAND BIOTECH INC
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