Unlock instant, AI-driven research and patent intelligence for your innovation.

Device for concentrating sample on line during coupling of liquid chromatogram and mass spectrum

A liquid chromatography and mass spectrometry technology, applied in the field of sample processing in liquid chromatography, to achieve the effect of improving sensitivity, detection and determination capabilities

Inactive Publication Date: 2009-12-09
SINOMS BIOTECH CO LTD
View PDF0 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The invention relates to a device for concentrating samples on-line when liquid chromatography and mass spectrometry are used together, which is used to solve the problem of on-line concentration of trace proteins, can improve the sensitivity of mass spectrometry, improve the quality of spectrograms, and especially can improve the quality of cascade spectrograms

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Device for concentrating sample on line during coupling of liquid chromatogram and mass spectrum
  • Device for concentrating sample on line during coupling of liquid chromatogram and mass spectrum
  • Device for concentrating sample on line during coupling of liquid chromatogram and mass spectrum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Such as figure 1 As shown, a device for concentrating samples online when liquid chromatography and mass spectrometry is used, the device includes a base, a through hole 13 arranged on the base, a hydrophilic microchannel 14 arranged on the base and communicated with the through hole 13 and One end communicates with the liquid chromatographic fraction 11 , and the other end communicates with the through hole 13 . In this embodiment, the through hole 13 may not be provided, and the other end of the connector 10 may directly communicate with the hydrophilic microchannel 14 .

[0017] The base is processed into a sheet shape by a piece of metal sheet with a thickness of 0.5mm, or in order to facilitate connection, the side of the metal sheet can be bent into a shape such as figure 1 As shown, similar to the tip of a pen, the metal sheet has a tip and a micro-channel structure. There is a through hole with a diameter of 1mm in the middle of the metal sheet. microchannel (...

Embodiment 2

[0019] Such as figure 2 As shown, a device for concentrating samples on-line when liquid chromatography and mass spectrometry is used, the device includes a base, a through hole 43 arranged on the base, a hydrophilic microchannel 44 arranged on the base and communicated with the through hole 43 and One end communicates with the liquid chromatographic fraction 41 , and the other end communicates with the through hole 43 . In this embodiment, the through hole 43 may not be provided, and the other end of the connector 40 may directly communicate with the hydrophilic microchannel 44 .

[0020] The base is processed into a sheet shape by a piece of metal sheet with a thickness of 0.5mm, or in order to facilitate connection, the side of the metal sheet can be bent into a shape such as figure 1 As shown, similar to the tip of a pen, the metal sheet has a tip and a microchannel structure. There is a through hole with a diameter of 3mm in the middle of the metal sheet. The through ho...

Embodiment 3

[0023] Such as image 3 As shown, a device for concentrating samples online when liquid chromatography and mass spectrometry is used, the device includes a base, a hydrophilic microchannel 24 arranged on the base, and one end communicates with the liquid chromatography fraction 21, and the other end communicates with the hydrophilic microchannel 21. The connecting piece 20 communicated with the microchannel 24 .

[0024] The base is a microfluidic chip 23 made of PDMS (polydimethylsiloxane), on which a microchannel 24 whose cross-sectional area decreases successively from the direction away from the connector 20 is designed on the microfluidic chip 23, The length of the microchannel 24 is about 5 cm, and the microchannel is also provided with a tip. The cross section of the microchannel 24 is partly round or "V" shaped. The surface of the microchannel 24 is oxidized and treated to become a hydrophilic surface. The intersection of the upper surface of the microfluidic chip 23 ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to View More

Abstract

The invention provides a device for concentrating a sample on line during coupling of liquid chromatogram and mass spectrum, which comprises a base part, a hydrophilic micro passage and a connector, wherein, the hydrophilic micro passage is arranged on the base part; one end of the connector is communicated with a liquid chromatogram fraction, and the other end of the connector is communicated with the hydrophilic micro passage; and the device further comprises an infrared laser module which is used for heating the hydrophilic micro passage or a heat conducting ceramic which is arranged under the hydrophilic micro passage to realize heating. The device can be used for concentrating the sample on line during coupling, can improve the sensitivity of the mass spectrum, can obtain high-quality spectrograms, and can greatly improve the detection and measurement capacities in particular when the amount of the sample is less.

Description

technical field [0001] The invention relates to a sample processing technology for liquid chromatography and mass spectrometry, in particular to a device for realizing online concentration of samples when liquid chromatography and electrospray mass spectrometry are combined. Background technique [0002] The electrospray technology of mass spectrometry is a soft ionization technology, which was first proposed by Fenn et al. It uses a strong electrostatic field for ionization, which is suitable for the analysis of macromolecular substances such as proteins. Fenn also won the 2002 Nobel Prize in Chemistry for this contribution. [0003] With the development of proteomics, the use of liquid chromatography and mass spectrometry to separate and identify proteins has become the mainstream analytical method for proteomics separation and identification. In liquid chromatography-mass chromatography (LC-MS), liquid chromatography / Electrospray mass spectrometry (LC-ESI-MS) is an impo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/00
Inventor 聂磊徐国宾杨芃原
Owner SINOMS BIOTECH CO LTD