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Technique for preparing fugui ostealgia compound by microorganism fermentation method

A microbial fermentation method and process technology, which is applied in the directions of microorganism-based methods, microorganisms, and biochemical equipment and methods to achieve the effects of reducing drug toxicity and side effects, improving drug efficacy, and increasing yield

Inactive Publication Date: 2012-05-23
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]The main problems in the production of Fugui Gutong are: the extraction process is backward, the patient takes a large amount of medicine (6 tablets at a time, 3 times a day); the active ingredient is not clear , low content of active ingredients
Therefore, Fuguigutong produced by the traditional extraction process has large side effects and many drug contraindications, which limit the application of the drug.

Method used

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  • Technique for preparing fugui ostealgia compound by microorganism fermentation method
  • Technique for preparing fugui ostealgia compound by microorganism fermentation method
  • Technique for preparing fugui ostealgia compound by microorganism fermentation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The preparation technology of embodiment 1 fermentation method production Fugui Gutong compound

[0041] 1. Preparation of Aspergillus niger Suspension

[0042] 1) Prepare plate medium:

[0043] Weigh 45g of nutrient agar and 20g of glucose, add it to 1000ml of distilled water, heat to boil, and stir with a glass rod at the same time, after the nutrient agar and glucose are completely dissolved in water (the water can be properly replenished when heating), put it into a triangular flask, and use 8 layers of gauze Make a stopper to stop the bottle mouth, then seal it with 4 layers of newspaper and cotton thread, and put it in a vertical pressure steam sterilizer to sterilize at 121°C for 20 minutes.

[0044] Quickly pour about 15ml of culture medium into a sterilized petri dish, cover and shake the petri dish so that the culture medium is evenly distributed on the bottom of the petri dish, place it flat on the table, and solidify to become a plate culture medium.

[00...

Embodiment 2

[0057] Example 2 Determination of the total alkaloid content of Aconitum aconitum in the extract by ion-pair extraction spectrophotometry

[0058] Aconitine was used as the reference substance. Precisely measure 50 mL of the extract prepared according to the method described in Example 1 "2. Compound Fermentation", adjust the pH value to 9 by adding ammonia to the test solution, extract 4 times with ether (25, 20, 15, 10 mL), and combine ether layer, and evaporated to dryness on a water bath. Add 0.01mol / L hydrochloric acid solution to the residue in an appropriate amount to dissolve it, transfer it to a 10mL measuring bottle, add 0.01mol / L hydrochloric acid solution to dilute to the mark, and shake well to obtain the test solution.

[0059] Precisely measure a certain amount of aconitine reference substance solution or test solution in a separatory funnel, add 5.0 mL of citric acid-disodium hydrogen phosphate buffer solution with a pH value of 3.50, 2.0 mL of BCG test soluti...

Embodiment 3

[0060] Example 3 Determination of Paeoniflorin Content in Extract by High Performance Liquid Chromatography

[0061] Be the chromatographic column Diamonsil C18 (150mm * 4.6mm, 5 μm) of filler with octadecylsilane bonded silica gel; Acetonitrile-0.1% phosphoric acid solution (14: 86) is mobile phase; Detection wavelength is 230nm; Column temperature is 40 ℃. Injection volume: 20 μL.

[0062] Accurately weigh a certain amount of paeoniflorin reference substance, add methanol to dissolve, and dilute to the scale solution to make a solution containing 0.5 μg per 1 mL, shake well to obtain paeoniflorin reference substance solution.

[0063] Accurately measure 1.5mL of the extract under item 2 with a pipette, evaporate to dryness in a water bath at low temperature, add 10mL of methanol to the residue, sonicate for 10 minutes to dissolve, centrifuge for 20 minutes (1500r / min), and transfer the supernatant to a 25mL volumetric flask, and make up to the mark with methanol. That is,...

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Abstract

The invention relates to a technique for preparing a fugui ostealgia compound by a microorganism fermentation method, comprising the following steps: all medicinal material are weighed according to the proportion of the fugui ostealgia compound, sterilized after being soaked by distilled water, inoculated with aspergillus niger and continuously cultured, the fermented medicinal materials are heated, circulated, extracted and filtered to obtain an extracting solution, and the extracting solution is centrifuged, concentrated and kept constant volume. The fugui ostealgia compound prepared according to the technique improves the curative effect of the medicine, lowers the toxic or side effect of the medicine, has low cost and is suitable for large-scale production.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and in particular relates to a biological fermentation process and application of a traditional Chinese medicine compound Fugui Gutong compound (FGGT Decoction). Background technique [0002] Fugui Gutong is a compound formula with independent intellectual property rights in my country. The traditional preparation process is mainly: except cinnamon crushed into fine powder, the other seven herbs are decocted twice with water, combined with the decoction, filtered, and the filtrate is concentrated into a thick paste. Add cinnamon powder, mix well, and dry under reduced pressure. Its effects are warming yang and dispelling cold, nourishing qi and promoting blood circulation, reducing swelling and relieving pain, and is used for hypertrophic arthritis of the cervical spine and knee joints of yang deficiency and cold dampness. Symptoms are: local joint pain, difficulty in flexion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/714A61P19/02C12N1/14C12R1/685
Inventor 葛喜珍郑来丽林强田平芳
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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