Method for preparing eatable phycobiliprotein in laver
A phycobiliprotein, edible-grade technology, applied in the field of food processing, can solve the problems of high cost, inability to industrialized production, high extraction cost, etc., and achieve the effect of solving difficult preservation, enhancing novelty, and extending shelf life
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[0013] Example one:
[0014] Take 50g of seaweed, grind it to powder with a solid grinder (the powder particle size is required to be 3-5mm), add it to distilled water or buffer solution pH 6.8 at a solid-liquid ratio of 1:20, and add a small amount of cellulase and pectin. Enzyme. The enzymolysis solution is placed in a high-speed homogenizer for intermittent homogenization for 5 minutes. After homogenization, the resulting solution was allowed to stand overnight at 4°C, placed in a centrifuge at 4°C, 8000rpm, and centrifuged at a medium speed, and the supernatant was taken after 5 minutes. Add ammonium sulfate solid to the obtained supernatant to a saturation of 25%, centrifuge at 10000 rpm, and take the supernatant after 15 minutes. The supernatant was continuously added with ammonium sulfate solid to a saturation of 60%, and then centrifuged at a medium speed at 12000 rpm, and the precipitate was collected after 15 minutes. Then the obtained precipitate was dissolved in di...
Example Embodiment
[0015] Embodiment two:
[0016] Take 500g of laver, grind it to powder with a solid grinder (the powder particle size is required to be 3-5mm), and add a small amount of cellulase and pectinase to distilled water or buffer solution pH 7.0 with a solid-liquid ratio of 1:15 . The enzymolysis solution is placed in a high-speed homogenizer for intermittent homogenization for 20 minutes. After homogenization, the resulting solution was allowed to stand overnight at 4°C, placed in a centrifuge, centrifuged at 4°C, 10,000 rpm, and medium speed, and the supernatant was taken after 10 minutes. Add ammonium sulfate solid to the obtained supernatant until the saturation is 25%, centrifuge at 12000 rpm at medium speed, and take the supernatant after 18 minutes. The supernatant was continuously added with ammonium sulfate solid to a saturation of 60%, and then centrifuged at a medium speed at 15000 rpm, and the precipitate was collected after 15 minutes. Then the obtained precipitate was d...
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